| Box-Behnken响应面法优选红景天苷乳剂凝胶处方工艺并研究其对烫伤的治疗作用 |
| 投稿时间：2019-08-25 点此下载全文
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| 引用本文：李越,吴中宝,杨艳玲,胡等慧,王秀丽.Box-Behnken响应面法优选红景天苷乳剂凝胶处方工艺并研究其对烫伤的治疗作用[J].中国现代中药,2020,22(4):609-614 |
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| 基金项目:重庆市科研机构绩效激励引导专项（cstc2017jxjl-jbky120022，cstc2017jxjl-jbky120015） |
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| 中文摘要:目的：优化红景天苷乳剂凝胶的处方工艺，及其对大鼠Ⅱ度烫伤的治疗作用，探讨其主要作用机制。方法：以卡波姆用量、甘油用量和pH为考察因素，以凝胶剂的外观、延展性、均匀度、离心性的综合评价为指标，采用Box-Behnken响应面法优选红景天苷乳剂凝胶的处方工艺。建立大鼠深Ⅱ度皮肤烫伤模型，分组给药，连续21 d，计算烫伤面积和愈合率并做皮肤组织病理观察，测定表皮生长因子（EGF）和碱性成纤维细胞生长因子（bFGF）的表达。结果：优选所得红景天苷乳剂凝胶质量稳定、重现性好。治疗过程中，给药组与模型组比较，愈合明显，表皮层、真皮层和基底层的炎细胞明显减少、肉芽组织增生明显，EGF、bFGF在各烫伤皮肤全层组织中表达均有显著性增加（P<0.05）。结论：红景天苷乳剂凝胶制备工艺简单，方法稳定可靠；红景天苷对烫伤修复有一定的治疗作用，其促进烫伤愈合的机理可能与烫伤组织中的EGF、bFGF有关；为扩展红景天苷治疗领域及新的给药形式提供参考。 |
| 中文关键词:红景天苷 乳剂 凝胶 烫伤 表皮生长因子 碱性成纤维细胞生长因子 |
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| Optimization of Formulation of Salidroside Emulsion gel by Box-Behnken Response Surface Methodology and Pharmacodynamic Study on Wound Healing |
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| Abstract:Objective：To optimize the formulation technology of salidroside emulsion gel and study its repair effect on scald wound in rats，and explore its main mechanism.Methods：Taking carbomer dosage，glycerol dosage and pH value as the investigation factors，selecting the comprehensive evaluation of gel appearance，ductility，evenness and centrifugation as the index，the Box-Behnken response surface method was used to optimize the formulation technology of salidroside emulsion gel.The rat model of deep second degree skin scald was established and the wound area and healing rate were calculated for 21 days.The expression of epidermal growth factor （EGF） and basic fibroblast growth factor （bFGF） were measured.Results：The salidroside emulsion gel prepared by the optimum technology were stable and reproducible.In the course of treatment，compared with the model group，healing speed of defected regions in drug groups was faster than the of the model group，the inflammatory cells in epidermis，dermis and basal layer decreased significantly，granulation tissue proliferated obviously，and the expressions of EGF and bFGF increased significantly in the whole skin tissue of each wound （P<0.05）. Conclusion：The preparation of salidroside emulsion gel is simple，stable and reliable.It has been proved that salidroside has a certain therapeutic effect on scald repair.The mechanism of salidroside promoting scald healing may be related to EGF and bFGF in scald tissue.All these lay a foundation for expanding the field of salidroside treatment and new forms of administration. |
| keywords:salidroside emulsion gel wound healing EGF bFGF |
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