| 淫羊藿药材及饮片质量分析与标准制定 |
| 投稿时间：2020-03-17 点此下载全文
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| 引用本文：王常顺,段吉平,康帅,袁浩,刘永利,冯丽.淫羊藿药材及饮片质量分析与标准制定[J].中国现代中药,2020,22(7):1006-1015 |
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| 基金项目:国家药品标准提高项目（2016-026） |
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| 中文摘要:目的：基于淫羊藿药材与饮片质量状况与统计分析，修订现行质量标准淫羊藿含量测定方法与限度。方法：采用HPLC建立以淫羊藿苷为参照的一标多测法（QAMS）测定淫羊藿中4种黄酮醇苷类成分的含量测定方法，对104批样品含量进行统计分析。结果：朝藿定A、朝藿定B、朝藿定C的相对保留时间分别为0.73、0.81、0.90，校正因子分别为1.35、1.28、1.22。聚类分析显示，朝鲜淫羊藿单独聚为一类，方差分析结果显示，朝鲜淫羊藿与心叶淫羊藿、柔毛淫羊藿P值均小于0.01，表明朝鲜淫羊藿总黄酮醇苷含量与其他3种基原差异有统计学意义。结论：建立的含量测定方法准确可靠、重复性好，根据品种特点单独制定限度，使新修订的质量标准更加科学、合理、可行。 |
| 中文关键词:淫羊藿 一标多测 淫羊藿苷 朝藿定A 朝藿定B 朝藿定C |
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| Quality Analysis and Standard Formulation of Herba Epimedii and Decoction Pieces |
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| Abstract:Objective：Based on the quality status and statistical analysis，to revise the determination method and quality standard of Herba Epimedii and decoction pieces. Methods：High performance liquid chromatography was used to develop a method for the determination of four flavonoid glycosides in Herba Epimedii with icariin as reference. The contents of 104 samples were analyzed statistically. Results：The relative retention time was respectively 0.73，0.81，0.90，the correction factors were respectively 1.35，1.28，1.22. Cluster analysis showed that Epimedium koreanum clustered into a single species. Variance analysis showed that the P between E. koreanum and E. pubescens and E. brevicornu were both less than 0.01，showed total flavone alcohol glycoside content of E. koreanum was significantly different from that of other three species. Conclusion：The established method is accurate，reliable and reproducible，and the revised quality standard is scientific and reasonable. |
| keywords:Herba Epimedii QAMS icariin epimedin A epimedin B epimedin C |
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