黄花蒿新品种“研青一号”研究
投稿时间:2019-02-19     点此下载全文
引用本文:丁丹丹,马婷玉,陈士林,冷梁,黄盛群,谢刚,向丽.黄花蒿新品种“研青一号”研究[J].中国现代中药,2020,22(7):1072-1079
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作者中文名作者英文名单位中文名单位英文名E-Mail
丁丹丹 DING Dan-dan 中国中医科学院 中药研究所/北京市中药鉴定与安全性检测评估重点实验室,北京100700 Key Laboratory of Beijing for Identification and Safety Evaluation of Chinese Medicine,Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100700,China  
马婷玉 MA Ting-yu 中国中医科学院 中药研究所/北京市中药鉴定与安全性检测评估重点实验室,北京100700 Key Laboratory of Beijing for Identification and Safety Evaluation of Chinese Medicine,Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100700,China  
陈士林 CHEN Shi-lin 中国中医科学院 中药研究所/北京市中药鉴定与安全性检测评估重点实验室,北京100700 Key Laboratory of Beijing for Identification and Safety Evaluation of Chinese Medicine,Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100700,China  
冷梁 LENG Liang 中国中医科学院 中药研究所/北京市中药鉴定与安全性检测评估重点实验室,北京100700 Key Laboratory of Beijing for Identification and Safety Evaluation of Chinese Medicine,Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100700,China  
黄盛群 HUANG Sheng-qun 广西仙草堂制药有限责任公司,广西柳州545400 Guangxi Xiancaotang Pharmaceutical Co., Ltd.,Liuzhou 545400,China  
谢刚 XIE Gang 广西仙草堂制药有限责任公司,广西柳州545400 Guangxi Xiancaotang Pharmaceutical Co., Ltd.,Liuzhou 545400,China  
向丽 XIANG Li 中国中医科学院 中药研究所/北京市中药鉴定与安全性检测评估重点实验室,北京100700 Key Laboratory of Beijing for Identification and Safety Evaluation of Chinese Medicine,Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100700,China 向丽,副研究员,研究方向:中药资源;E-mail:lxiang@icmm.ac.cn 
基金项目:国家自然科学基金特别项目(81641002,U1812403-1);国家“重大新药创制”科技重大专项(2017ZX09101002-003-001,2019ZX09201005-006-001);中央级公益性科研院所基本科研业务费专项资金资助(ZZ10-024,ZZ13-YQ-102)
中文摘要:目的:通过培育青蒿素高含量的新品种,降低青蒿素提取成本,保障我国青蒿素原料供应优势地位。方法:通过混合选育获得青蒿素含量高的新品种“研青一号”,进行特异性、一致性、稳定性评价;对来自15个产地样品进行基因组重测序,筛选“研青一号”特征性插入缺失突变位点,并采用药用植物全球产地生态适宜性信息系统(GMPGIS)预测其全球适宜种植范围。结果:“研青一号”植株呈圆塔型,矮小、紧凑、叶片较大,生育期240~250 d,种子千粒质量(34.98±1.74) g,单株干叶片产量(211.90±48.07) g;青蒿素含量较高,平均为(2.11±0.38)%,最高达2.70%。2对AaIn101和AaIn110特异性引物,可用于区分 “研青一号”和其他产地黄花蒿种质。中国为“研青一号”适宜种植的主要区域,主要集中在我国东南部,以广西最大,达211 656.27 km2;结论:“研青一号”植株形态特征特异性明显,青蒿素含量较高;具有特异性、一致性及稳定性,适宜在我国东南部推广。
中文关键词:“研青一号”  黄花蒿  青蒿素  规范化栽培
 
Study on New Variety of Artemisia annua L. ″Yanqing No.1″
Abstract:Objective:To breed high artemisinin content varieties to reduce the extraction cost and guarantee China′s dominant position in supply of artemisinin. Methods:A high artemisinin content variety,″Yanqing No.1″,was obtained through mixed breeding methods and its specificity,consistency,and stability was evaluated. After genomic resequencing,the special insertion deletion mutations of ″Yanqing No.1″ were screened compared with other 14 samples. Global geographic information system for medicinal plant(GMPGIS)was also used to predict its global suitable planting areas. Results:The ″Yanqing No.1″(YQ 1) variety is a round tower type,short,compact,large in leaves,and has a growth period of 240-250 days. The thousand seeds weight is (34.98±1.74)g,and the dry leaf yield per plant is (211.90±48.07) g. The average content of artemisinin is (2.11±0.38)%,and the highest is 2.70%. Two pairs specific primers of AaIn101 and AaIn110 were screened to distinguish YQ1 and other 14 samples. The global suitable planting area of ″Yanqing No.1″ is mainly in China. The suitable planting areas in China is mainly concentrated in the southeast,with the largest in Guangxi,reaching 211 656.27 km2. Conclusion:The ″Yanqing No.1″ plant has specific morphological characteristics and high artemisinin content with specificity,consistency and stability and is suitable for planting in the southeast of China.
keywords:″Yanqing No.1″  Artemisia annua  artemisinin  standardized cultivation
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