地黄叶片响应遮阴处理的分子响应机制解析
投稿时间:2019-08-27     点此下载全文
引用本文:李明杰,谢彩侠,古力,杨超飞,杜家方,王丰青,张重义.地黄叶片响应遮阴处理的分子响应机制解析[J].中国现代中药,2020,22(7):1080-1089
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作者中文名作者英文名单位中文名单位英文名E-Mail
李明杰 LI Ming-jie 福建农林大学 农学院,福建福州350002 College of Agriculture,Fujian Agriculture and Forestry University,Fuzhou 350002,China  
谢彩侠 XIE Cai-xia 福建农林大学 农学院,福建福州350002 College of Agriculture,Fujian Agriculture and Forestry University,Fuzhou 350002,China  
古力 GU Li 福建农林大学 农学院,福建福州350002 College of Agriculture,Fujian Agriculture and Forestry University,Fuzhou 350002,China  
杨超飞 YANG Chao-fei 河南农业大学 农学院,河南郑州450002 College of Agronomy
Henan Agricultural University,Zhengzhou 450002,China
 
杜家方 DU Jia-fang 河南农业大学 农学院,河南郑州450002 College of Agronomy
Henan Agricultural University,Zhengzhou 450002,China
 
王丰青 WANG Feng-qing 河南农业大学 农学院,河南郑州450002 College of Agronomy
Henan Agricultural University,Zhengzhou 450002,China
王丰青,副教授,研究方向:中药资源分子生物技术与新品种选育;Tel:(0371)56990188,E-mail:heauzycxw@126.com 
张重义 ZHANG Zhong-yi 福建农林大学 农学院,福建福州350002 College of Agriculture,Fujian Agriculture and Forestry University,Fuzhou 350002,China 张重义,教授,研究方向:中药资源生态与中药材生产技术;Tel:(0591)83742793,E-mail:zyzhang@fafu.edu.cn 
基金项目:国家重点研发计划项目(2017YFC1700705);国家自然科学基金项目(81473299,81872950,81603243)
中文摘要:目的:分析遮阴后地黄叶片中差异表达的基因,揭示地黄响应遮阴的分子机制。方法:对地黄“温85-5”植株进行60%遮阴、90%遮阴和全光照3种处理,以Ion Proton平台对90%遮阴和全光照2种处理的样品进行转录组测序,筛选差异表达的基因。结果:遮阴后地黄叶片变平展,泡状凸起减少或变小,叶片变薄,叶肉栅栏组织和海绵组织细胞减少、变小。同时,有2393个基因在遮阴处理的叶片中差异表达,其中,遮阴后上调表达的基因有1456个,而下调的基因有937个。京都基因与基因组百科全书(KEGG)富集分析发现植物-真菌互作代谢通路、苯乙醇苷生物合成通路、萜类成分合成通路、光合作用通路等120个代谢通路相关的差异基因得到了富集。在遮阴后的叶片中,参与光合作用的差异基因全部下调表达,生长素响应蛋白基因(ARF)上调表达,赤霉素(GA)通路DELLA基因下调表达,乙烯通路苏氨酸蛋白激酶基因(CTR1)上调表达,参与梓醇与毛蕊花糖苷合成通路的催化酶基因表达与它们遮阴后含量的变化规律也有一定的相关性。结论:分析了遮阴后地黄叶片的转录组信息,筛选到一些差异表达的基因,为进一步解析地黄遮阴响应的分子机制奠定基础。
中文关键词:地黄  遮阴  叶片  差异基因
 
Analysis of Molecular Mechanisms of Rehmannia glutinosa Leaf in Response to Shading Treatment
Abstract:Objective:To reveal the molecular regulation of Rehmannia glutinosa leaf in response to shading,and explore key genes related to shading resistance. Methods:High through-put transcriptome sequencing by Ion Proton was performed to screen differentially expressed genes (DEGs) using leaves of ‘Wen 85-5’ under full light,60% shading and 90% shading treatments. Results:Shading lessened bulges of R. glutinosa leaves,decreased the thickness of leaves,and reduced the cell number of palisade and spongy tissues. Totally,2393 DEGs were determined between full light vs. 90% shading,with 1456 up-regulated genes and 937 down-regulated genes. These DEGs with a KEGG pathway annotation were enriched into 120 KEGG pathways,including plant-pathogen interaction,phenylpropanoid biosynthesis,terpenoid biosynthesis and photosynthesis,etc. In 90% shading leaves,all of differentially expressed genes involved in photosynthesis were down-regulated,nine of auxin response factor genes involved in auxin signaling pathway were up-regulated,ten DELLA genes involved in GA signaling pathway were down-regulated,and five CTR1 genes involved in ethylene signaling pathway were up-regulated. After shading treated,the expression changes of enzyme genes involved in catapol and acteoside biosynthesis were related to their accumulation. Conclusion:In this study,we identified some key genes after shading treated by RNA-Seq,which will lay foundation to elucidate the molecular regulation of R. glutinosa leaf in response to shading.
keywords:Rehmannia glutinosa  shading  leaf  differentially expressed genes
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