| HPLC测定小儿柴葛口服液原方中10种成分含量 |
| 投稿时间：2019-08-28 点此下载全文
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| 引用本文：丁鹏敏,刘斯琪,庞月笙,王莎,玄振玉,张彤,王如峰.HPLC测定小儿柴葛口服液原方中10种成分含量[J].中国现代中药,2020,22(7):1118-1123 |
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| 基金项目:国家科技重大专项（2019ZX09201004-002） |
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| 中文摘要:目的：建立小儿柴葛口服液原方中3′-羟基葛根素、3′-甲氧基葛根素、葛根素、芍药苷、芍药内酯苷、汉黄芩苷、黄芩苷、甘草苷、柴胡皂苷a、柴胡皂苷b2的含量测定方法。方法：采用Agilent TC-C18色谱柱（250 mm×4.6 mm，5 μm），流动相为乙腈（A）-0.1%磷酸水溶液（B），梯度洗脱，流速为1.0 mL·min-1，柱温为30 ℃，检测波长为230、210 nm。结果：该方法的专属性良好，精密度、重复性和稳定性的RSD均小于2%；3′-羟基葛根素、3′-甲氧基葛根素、葛根素、芍药苷、芍药内酯苷、汉黄芩苷、黄芩苷、甘草苷、柴胡皂苷a、柴胡皂苷b2分别在0.005~0.078、0.010~0.163、0.038~0.603、0.014~0.226、0.004~0.052、0.011~0.170、0.049~0.780、0.003~0.046、0.006~0.092、0.002~0.036 mg·mL-1呈良好线性关系；在测定的3批样品中上述化合物的平均质量分数分别为1.41、2.85、11.06、3.47、1.35、2.88、13.60、0.73、3.13、1.18 mg·g-1。结论：该方法简便，易操作，重复性好，可用于小儿柴葛口服液原方中10种主要成分的含量测定。 |
| 中文关键词:小儿柴葛口服液 高效液相色谱法 含量测定 质量控制 |
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| Content Assay of 10 Components in Original Prescription of Xiaoer Chaige Oral Solution by HPLC |
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| Abstract:Objective：To establish a content assay method for 3′-hydroxypuerarin，3′-methoxypuerarin，puerarin，paeoniflorin，albiflorin，wogonoside，baicalin，liquiritin，saikosaponin a and saikosaponin b2 in the original prescription of Xiaoer Chaige Oral Solution. Methods：HPLC analysis was performed on an Agilent TC-C18 column （250 mm×4.6 mm，5 μm） with gradient elution using acetonitrile as mobile phase A and 0.1% aqueous phosphoric acid as mobile phase B at the flow rate of 1.0 mL·min-1 and column temperature of 30 ℃ under detection wavelength of 230 nm and 210 nm. Results：The method was specific，and its RSDs for precision，repeatability and stability were all less than 2%. 3′-hydroxypuerarin，3′-methoxypuerarin，puerarin，paeoniflorin，albiflorin，wogonoside，baicalin，liquiritin，saikosaponin a and saikosaponin b2 showed good linear relationship in the range of 0.005-0.078，0.010-0.163，0.038-0.603，0.014-0.226，0.004-0.052，0.011-0.170，0.049-0.780，0.003-0.046，0.006-0.092 and 0.002-0.036 mg·mL-1. The average contents of the above compounds in three batches of samples determined were 1.41，2.85，11.06，3.47，1.35，2.88，13.60，0.73，3.13 and 1.18 mg·g-1，respectively. Conclusion：This method is simple，easy to operate and well repeatable，thereby it can be used for the content determination of ten major components in the original prescription of Xiaoer Chaige Oral Solution. |
| keywords:Xiaoer Chaige Oral Solution HPLC content determination quality control |
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