钩吻属植物及其易混品五指毛桃的DNA条形码比较分析
投稿时间:2020-03-15     点此下载全文
引用本文:王虹,苏畅,张文娴,刘雪婷,吕浩锋,王铁杰,魏锋,马双成,王淑红.钩吻属植物及其易混品五指毛桃的DNA条形码比较分析[J].中国现代中药,2020,22(12):1967-1971
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作者中文名作者英文名单位中文名单位英文名E-Mail
王虹 WANG Hong 深圳市药品检验研究院,广东深圳518057 Shenzhen Institute for Drug Control,Shenzhen 518057, China  
苏畅 SU Chang 深圳市药品检验研究院,广东深圳518057 Shenzhen Institute for Drug Control,Shenzhen 518057, China  
张文娴 ZHANG Wen-xian 深圳市药品检验研究院,广东深圳518057 Shenzhen Institute for Drug Control,Shenzhen 518057, China  
刘雪婷 LIU Xue-ting 深圳市药品检验研究院,广东深圳518057 Shenzhen Institute for Drug Control,Shenzhen 518057, China  
吕浩锋 LYU Hao-feng 深圳市药品检验研究院,广东深圳518057 Shenzhen Institute for Drug Control,Shenzhen 518057, China  
王铁杰 WANG Tie-jie 深圳市药品检验研究院,广东深圳518057 Shenzhen Institute for Drug Control,Shenzhen 518057, China  
魏锋 WEI Feng 中国食品药品检定研究院 中药民族检定所,北京100050 Institute for Control of Chinese Traditional Medicine and Ethnic Medicine,National Institutes for Food and Drug Control,Beijing 100050,China  
马双成 MA Shuang-cheng 中国食品药品检定研究院 中药民族检定所,北京100050 Institute for Control of Chinese Traditional Medicine and Ethnic Medicine,National Institutes for Food and Drug Control,Beijing 100050,China  
王淑红 WANG Shu-hong 深圳市药品检验研究院,广东深圳518057 Shenzhen Institute for Drug Control,Shenzhen 518057, China  
基金项目:深圳市科技计划项目(CXZZ20150529151451703)
中文摘要:目的:对钩吻属植物钩吻和北美钩吻进行2种DNA条形码的碱基序列分析,结合药食同源品种五指毛桃构建系统进化树,为五指毛桃药材的真伪鉴定提供参考。方法:对钩吻、北美钩吻和五指毛桃样品进行内部转录间隔区2(ITS2)序列和psbA-trnH序列的扩增测序,比对分析2种DNA条形码扩增产物碱基组成、GC含量、差异位点的差异;通过构建Kimura 2-parameter(K2P)双参数模型计算和分析种内、种间遗传距离;基于邻接法构建系统进化树,分析ITS2序列和psbA-trnH序列的鉴定效率。结果:ITS2区和psbA-trnH区序列均可作为钩吻属植物与五指毛桃鉴定的DNA条形码序列。其中psbA-trnH区在钩吻属种间的碱基序列长度、种间变异位点数量均具有显著差异,系统进化树的聚类结果稳定性更强,比ITS2更适用于该属植物样本的鉴别。结论:psbA-trnH序列可作为钩吻和北美钩吻鉴定首选DNA条形码,ITS2序列可作为五指毛桃与钩吻属植物鉴定的首选DNA条形码。
中文关键词:钩吻  北美钩吻  五指毛桃  DNA条形码  内转录间隔区  psbA-trnH
 
Comparative Analysis of Gelsemium and Its Confused Traditional Chinese Medicine Ficus hirta Base on DNA Barcodes
Abstract:Objective:To analyze molecular characteristics of Gelsemium elegans and G.sempervirens based on two types of DNA barcoding sequences,and to develop authentic method for Ficus hirta from these two highly venomous species using phylogenetic cluster analysis. Methods:The second internal transcribed spacer (ITS2) and psbA-trnH sequences were amplified and sequenced. Base composition,GC content and differential sites of different DNA barcodes were compared,and the Kimura 2-parameter (K2P) model was used to calculate the intraspecific and interspecific distances. Finally,two neighbor-joining phylogenetic trees were constructed based on different DNA barcodes. Results:ITS2 and psbA-trnH sequences could be used as DNA barcoding sequences for the identification of the Gelsemium species and F.hirta.PsbA-trnH sequences presented more significant differences in the length of PCR product sequence and the number of interspecific variation sites between the two Gelsemium species,as well as the highly repeatable results in phylogenetic clustering tree. However,ITS2 was still the reliable and prior barcode for the identification of materia medica samples of these species. Conclusion:psbA-trnH sequence could be the prior DNA barcode for the botanical identification of G.elegans and G.sempervirens. And ITS2 sequence could be the prior identical DNA barcode for F.hirta and the confused Gelscmium species.
keywords:Gelsemium elegans (Gardn.et Champ.) Benth.  G.sempervirens (L.) J.St.-Hil.  Ficus hirta Vahl.  DNA barcode  ITS2  psbA-trnH
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