消癌解毒方黄酮类成分及其给药后在大鼠体内分布
投稿时间:2019-11-14     点此下载全文
引用本文:钱桂英,闫秋莹,蒋家璐,范汇森,谭佳妮,程海波,孙东东.消癌解毒方黄酮类成分及其给药后在大鼠体内分布[J].中国现代中药,2020,22(12):1977-1984
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作者中文名作者英文名单位中文名单位英文名E-Mail
钱桂英 QIAN Gui-ying 南京中医药大学 常熟附属医院,江苏苏州215500 Changshu Hospital Affiliated to Nanjing University of Chinese Medicine,Suzhou 215500,China  
闫秋莹 YAN Qiu-ying 国家中医药管理局 名医验方评价与转化重点研究室/江苏省抗肿瘤验方研究与产业化工程实验室/江苏省中医药防治肿瘤协同创新中心/南京中医药大学 转化医学研究中心,江苏南京210023 Key Laboratory of SATCM for Empirical Formulae Evaluation and Achievements Transformation,Jiangsu Province Laboratory of Anti-cancer Empirical Formulae Research and Industrialization,Collaborative Innovation Center of Jiangsu Province Chinese Medicine in Cancer Prevention and Treatment,Translational Medicine Research Center of Nanjing University of Chinese Medicine,Nanjing 210023,China  
蒋家璐 JIANG Jia-lu 南京中医药大学 药学院,江苏南京210023 College of Pharmacy,Nanjing University of Chinese Medicine,Nanjing 210023,China  
范汇森 FAN Hui-sen 南京中医药大学 药学院,江苏南京210023 College of Pharmacy,Nanjing University of Chinese Medicine,Nanjing 210023,China  
谭佳妮 TAN Jia-ni 国家中医药管理局 名医验方评价与转化重点研究室/江苏省抗肿瘤验方研究与产业化工程实验室/江苏省中医药防治肿瘤协同创新中心/南京中医药大学 转化医学研究中心,江苏南京210023 Key Laboratory of SATCM for Empirical Formulae Evaluation and Achievements Transformation,Jiangsu Province Laboratory of Anti-cancer Empirical Formulae Research and Industrialization,Collaborative Innovation Center of Jiangsu Province Chinese Medicine in Cancer Prevention and Treatment,Translational Medicine Research Center of Nanjing University of Chinese Medicine,Nanjing 210023,China  
程海波 CHENG Hai-bo 国家中医药管理局 名医验方评价与转化重点研究室/江苏省抗肿瘤验方研究与产业化工程实验室/江苏省中医药防治肿瘤协同创新中心/南京中医药大学 转化医学研究中心,江苏南京210023 Key Laboratory of SATCM for Empirical Formulae Evaluation and Achievements Transformation,Jiangsu Province Laboratory of Anti-cancer Empirical Formulae Research and Industrialization,Collaborative Innovation Center of Jiangsu Province Chinese Medicine in Cancer Prevention and Treatment,Translational Medicine Research Center of Nanjing University of Chinese Medicine,Nanjing 210023,China  
孙东东 SUN Dong-dong 国家中医药管理局 名医验方评价与转化重点研究室/江苏省抗肿瘤验方研究与产业化工程实验室/江苏省中医药防治肿瘤协同创新中心/南京中医药大学 转化医学研究中心,江苏南京210023 Key Laboratory of SATCM for Empirical Formulae Evaluation and Achievements Transformation,Jiangsu Province Laboratory of Anti-cancer Empirical Formulae Research and Industrialization,Collaborative Innovation Center of Jiangsu Province Chinese Medicine in Cancer Prevention and Treatment,Translational Medicine Research Center of Nanjing University of Chinese Medicine,Nanjing 210023,China 孙东东,副研究员,研究方向:抗肿瘤中药物质基础;Tel:(025)85811071,E-mail:sun_21373@163.com 
基金项目:国家自然科学基金项目(81673559,81573910);国家自然科学基金青年基金项目(81804073);江苏省自然科学基金项目(BK20161045);江苏省高校自然基金面上项目(16KJB360001)
中文摘要:目的:建立超高效液相色谱-串联四极杆飞行时间质谱法(UPLC-Q-TOF-MS)检测消癌解毒方水提液中黄酮类成分的方法,同时研究大鼠灌胃给予消癌解毒方水提液后黄酮类成分在体内的分布情况。方法:采用Agilent C18(100 mm×2.1 mm,1.8 μm)反相色谱柱对各待测物进行分离,以乙腈-0.1%甲酸水溶液为流动相梯度洗脱,应用电喷雾离子源(ESI),在正、负离子模式下采集数据。结果:从消癌解毒方水提液中共鉴定出23个黄酮类化学成分;大鼠经灌胃给予消癌解毒方水提液后,6-甲氧基柚皮素(13)、柚皮素(14)、4′-羟基汉黄芩素(15)、芹菜素(16)、汉黄芩素(19)在多个生物样品中被检测发现,其中在肠、脑、胃、粪便样品中分别检测出6、6、12、10个黄酮类成分。结论:建立的方法可快速分析鉴定出消癌解毒方水提液中及给药后大鼠有关部位中黄酮类成分,为进一步阐释复方中特征化学成分及其在体内的变化分布规律提供参考。
中文关键词:消癌解毒方  超高效液相色谱-串联四极杆飞行时间质谱法  黄酮  化学成分  体内分布
 
Identification of Flavonoids in Xiaoai Jiedu Decoction and Its Distribution in Rats
Abstract:Objective:Ultra-high performance liquid chromatography-tandem-quadrupole-time of fligh-mass spectrometry (UPLC-Q-TOF-MS) was used to identify the flavonoids in Xiaoai Jiedu Decoction (XJD) water extract and their distribution in rats after oral administration of XJD. Methods:The analytes were separated on an Agilent C18 column (100 mm×2.1 mm,1.8 μm),and eluted with a gradient of acetonitrile-water containing 0.1% formic acid. The mass spectrometer equipped with electron spray ionization (ESI) was used as detector and operated under the positive and negative ion mode. Results:23 flavonoids were isolated and identified. Among them,compounds 6-methoxynaringenin (13),naringenin (14),4′-hydroxwogonin (15),apigenin (16),wogonin (19) were detected in multiple biological samples. Among them,6,6,12,and 10 species flavonoids were detected in intestinal,brain,stomach,and stool samples,respectively. Conclusion:The liquid-mass method established in this experiment can quickly analyze and identify the flavonoids in XJD and the flavonoids in the effective parts of rats after oral administration of XJD.
keywords:Xiaoai Jiedu Decoction  UPLC-Q-TOF-MS  flavonoids  chemical composition  distribution  in vivo
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