Determination of Three Non-alkaloids from Sophorae Tonkinensis Radix et Rhizoma with Quantitative Analysis of Multi-components by Single Marker
Abstract:Objective：To establish a quantitative analysis of multi-components by single marker （QAMS） method for maackiain，trifolirhizin and tonkinensisol in Sophorae Tonkinensis Radix et Rhizoma，in order to provide reference for the quality evaluation of this species. Methods：With maackiain as internal reference，the relative correction factors of trifolirhizin and tonkinensisol were calculated to verify the feasibility and applicability of QAMS method. The content of the three components were measured and compared with the external standard method （ESM）. Results：The QAMS of Sophorae Tonkinensis Radix et Rhizoma was established. Compared to maackiain，the relative correction factors of trifolirhizin and tonkinensisol are 1.609 and 0.774，respectively. The factor was reproducible，and there was no significant difference among the values measured by the ESM method. Conclusion：The established QAMS method shows good precision，stability and repeatability，and can be used for the quantitative analysis of maackiain，trifolirhizin and tonkinensisol，the non-alkaloids of Sophorae Tonkinensis Radix et Rhizoma.
