一测多评法测定山豆根中3个非生物碱成分含量
投稿时间:2019-11-25     点此下载全文
引用本文:黄乐,姚彩云,梁莹,闫炳雄,刘喜慧,吴云秋,阮丽君,缪剑华,宋志军.一测多评法测定山豆根中3个非生物碱成分含量[J].中国现代中药,2020,22(12):2015-2020
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作者中文名作者英文名单位中文名单位英文名E-Mail
黄乐 HUANG Le 广西中医药大学 药学院,广西南宁530200 Guangxi University of Chinese Medicine,Nanning 530200,China  
姚彩云 YAO Cai-yun 广西壮族自治区药用植物园 西南濒危药材资源开发国家工程实验室,广西南宁530023 College of Pharmacy,National Engineering Laboratory of Southwest Endangered Medicinal Resources Development,Guangxi Botanical Garden of Medicinal Plant,Nanning 530023,China  
梁莹 LIANG Ying 广西壮族自治区药用植物园 西南濒危药材资源开发国家工程实验室,广西南宁530023 College of Pharmacy,National Engineering Laboratory of Southwest Endangered Medicinal Resources Development,Guangxi Botanical Garden of Medicinal Plant,Nanning 530023,China  
闫炳雄 YAN Bing-xiong 广西壮族自治区药用植物园 西南濒危药材资源开发国家工程实验室,广西南宁530023 College of Pharmacy,National Engineering Laboratory of Southwest Endangered Medicinal Resources Development,Guangxi Botanical Garden of Medicinal Plant,Nanning 530023,China  
刘喜慧 LIU Xi-hui 广西壮族自治区药用植物园 西南濒危药材资源开发国家工程实验室,广西南宁530023 College of Pharmacy,National Engineering Laboratory of Southwest Endangered Medicinal Resources Development,Guangxi Botanical Garden of Medicinal Plant,Nanning 530023,China  
吴云秋 WU Yun-qiu 广西壮族自治区药用植物园 西南濒危药材资源开发国家工程实验室,广西南宁530023 College of Pharmacy,National Engineering Laboratory of Southwest Endangered Medicinal Resources Development,Guangxi Botanical Garden of Medicinal Plant,Nanning 530023,China  
阮丽君 RUAN Li-jun 广西壮族自治区药用植物园 西南濒危药材资源开发国家工程实验室,广西南宁530023 College of Pharmacy,National Engineering Laboratory of Southwest Endangered Medicinal Resources Development,Guangxi Botanical Garden of Medicinal Plant,Nanning 530023,China  
缪剑华 MIAO Jian-hua 广西中医药大学 药学院,广西南宁530200 Guangxi University of Chinese Medicine,Nanning 530200,China 缪剑华,研究员,研究方向:药用植物生态学及中药资源经济学;Tel:(0771)5611352,E-mail:mjh1962@vip.163.com 
宋志军 SONG Zhi-jun 广西壮族自治区药用植物园 西南濒危药材资源开发国家工程实验室,广西南宁530023 College of Pharmacy,National Engineering Laboratory of Southwest Endangered Medicinal Resources Development,Guangxi Botanical Garden of Medicinal Plant,Nanning 530023,China 宋志军,副研究员,研究方向:天然药物化学成分研究;Tel:(0771)5602461,E-mail:88519368@qq.com 
基金项目:国家中医药行业科研专项(201507002);国家中药材产业技术体系(CARS-21);广西创新驱动发展专项资金项目(桂科AA18242040);广西壮族自治区药用植物园创新团队项目(桂药创2019006)
中文摘要:目的:建立山豆根中高丽槐素、红车轴草苷和越南槐醇3个成分含量的一测多评方法,为山豆根的质量评价提供参考。方法:以高丽槐素为内参物,计算红车轴草苷、越南槐醇相对校正因子,利用相对校正因子计算2个成分的含量,同时与外标法测定结果对比,验证一测多评法的可行性和适用性。结果:建立了以高丽槐素为内参物,红车轴草苷和越南槐醇的相对校正因子分别为1.609和0.774的山豆根药材多成分一测多评定量方法,相对校正因子重复性良好,与外标法测得值之间差异无统计学意义。结论:建立的一测多评方法精密度、稳定性及重复性较好,可用于山豆根中高丽槐素、红车轴草苷、越南槐醇3种非生物碱成分的定量分析。
中文关键词:山豆根  一测多评  质量评价  红车轴草苷  高丽槐素  越南槐醇
 
Determination of Three Non-alkaloids from Sophorae Tonkinensis Radix et Rhizoma with Quantitative Analysis of Multi-components by Single Marker
Abstract:Objective:To establish a quantitative analysis of multi-components by single marker (QAMS) method for maackiain,trifolirhizin and tonkinensisol in Sophorae Tonkinensis Radix et Rhizoma,in order to provide reference for the quality evaluation of this species. Methods:With maackiain as internal reference,the relative correction factors of trifolirhizin and tonkinensisol were calculated to verify the feasibility and applicability of QAMS method. The content of the three components were measured and compared with the external standard method (ESM). Results:The QAMS of Sophorae Tonkinensis Radix et Rhizoma was established. Compared to maackiain,the relative correction factors of trifolirhizin and tonkinensisol are 1.609 and 0.774,respectively. The factor was reproducible,and there was no significant difference among the values measured by the ESM method. Conclusion:The established QAMS method shows good precision,stability and repeatability,and can be used for the quantitative analysis of maackiain,trifolirhizin and tonkinensisol,the non-alkaloids of Sophorae Tonkinensis Radix et Rhizoma.
keywords:Sophorae Tonkinensis Radix et Rhizoma  QAMS  quality evaluation  maackiain  trifolirhizin  tonkinensisol
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