复方鱼腥草合剂的HPLC指纹图谱建立
投稿时间:2019-11-04     点此下载全文
引用本文:应珂,王建方,查慧军,郑亚纯,金汉台,姚建标.复方鱼腥草合剂的HPLC指纹图谱建立[J].中国现代中药,2020,22(12):2059-2062
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作者中文名作者英文名单位中文名单位英文名E-Mail
应珂 YING Ke 浙江康恩贝制药股份有限公司/浙江省中药制药技术重点实验室,浙江杭州310052 Zhejiang CONBA Pharmaceutical Co.,Ltd.,Zhejiang Provincial Key Laboratory of Traditional Chinese Medicine Pharmaceutical Technology,Hangzhou 310052,China  
王建方 WANG Jian-fang 浙江康恩贝制药股份有限公司/浙江省中药制药技术重点实验室,浙江杭州310052 Zhejiang CONBA Pharmaceutical Co.,Ltd.,Zhejiang Provincial Key Laboratory of Traditional Chinese Medicine Pharmaceutical Technology,Hangzhou 310052,China  
查慧军 ZHA Hui-jun 浙江康恩贝制药股份有限公司/浙江省中药制药技术重点实验室,浙江杭州310052 Zhejiang CONBA Pharmaceutical Co.,Ltd.,Zhejiang Provincial Key Laboratory of Traditional Chinese Medicine Pharmaceutical Technology,Hangzhou 310052,China  
郑亚纯 ZHENG Ya-chun 浙江康恩贝制药股份有限公司/浙江省中药制药技术重点实验室,浙江杭州310052 Zhejiang CONBA Pharmaceutical Co.,Ltd.,Zhejiang Provincial Key Laboratory of Traditional Chinese Medicine Pharmaceutical Technology,Hangzhou 310052,China  
金汉台 JIN Han-tai 浙江康恩贝中药有限公司,浙江丽水323400 Zhejiang CONBA Chinese Medicine Co.,Ltd.,Lishui 323400,China  
姚建标 YAO Jian-biao 浙江康恩贝制药股份有限公司/浙江省中药制药技术重点实验室,浙江杭州310052 Zhejiang CONBA Pharmaceutical Co.,Ltd.,Zhejiang Provincial Key Laboratory of Traditional Chinese Medicine Pharmaceutical Technology,Hangzhou 310052,China 姚建标,高级工程师,研究方向:中药研发;E-mail:yaojb@conbagroup.com 
基金项目:浙江省重大科技专项重点社会发展项目(2013C03005)
中文摘要:目的:建立复方鱼腥草合剂的HPLC指纹图谱,实现对其质量进行整体高精度的控制。方法:以Besil 5 C18-B(250 mm×4.6 mm,5 μm)为色谱柱,以甲醇为流动相A,以0.1%磷酸溶液为流动相B,梯度洗脱;检测波长为210 nm,柱温为40 ℃,进样量为5 μL,流速为1.0 mL·min-1。建立复方鱼腥草合剂的指纹图谱,并通过对照品和液相色谱-质谱法(LC-MS)对共有峰进行指认和归属。检测3个厂家15批样品,并采用“中药色谱指纹图谱相似度评价系统”软件进行分析。结果:建立了复方鱼腥草合剂的HPLC指纹图谱,标定了16个共有峰,15批样品相似度均不低于0.95。结论:该方法样品处理简便,色谱条件简单、结果准确、重复性好,可以作为复方鱼腥草合剂质量控制的方法之一。
中文关键词:复方鱼腥草合剂  指纹图谱  高效液相色谱  黄芩苷
 
Fingerprints Analysis of Compound Yuxingcao Mixture by HPLC
Abstract:Objective:To establish an HPLC for fingerprints analysis of compound Yuxingcao Mixture for its overall high accuracy quality control. Methods:The HPLC analysis was performed on Besil 5 C18-B (250 mm×4.6 mm,5 μm) with the mobile phase of methanol-0.1% phosphoric acid with gradient elution at the flow rate of 1.0 mL·min-1. The detection wavelength was set at 210 nm,the column temperature was at 40 ℃,and the injection volume was 5 μL. The common peaks were identified and attributed by combining the reference substance with LC-MS. 15 batches of samples which were made by three different manufacturers were determined and analyzed by Similarity Evaluation System for chromatographic Fingerprint of TCM. Results:The HPLC fingerprint of compound Yuxingcao Mixture was established. Totally 16 common characteristic peaks were identified. And the similarity of HPLC fingerprints was above 0.95 for all samples. Conclusion:The method is simple and repeatable,which can be used for the quality control of compound Yuxingcao Mixture.
keywords:compound Yuxingcao Mixture  fingerprints  HPLC  baicalin
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