| 复方鱼腥草合剂的HPLC指纹图谱建立 |
| 投稿时间：2019-11-04 点此下载全文
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| 引用本文：应珂,王建方,查慧军,郑亚纯,金汉台,姚建标.复方鱼腥草合剂的HPLC指纹图谱建立[J].中国现代中药,2020,22(12):2059-2062 |
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| 基金项目:浙江省重大科技专项重点社会发展项目（2013C03005） |
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| 中文摘要:目的：建立复方鱼腥草合剂的HPLC指纹图谱，实现对其质量进行整体高精度的控制。方法：以Besil 5 C18-B（250 mm×4.6 mm，5 μm）为色谱柱，以甲醇为流动相A，以0.1%磷酸溶液为流动相B，梯度洗脱；检测波长为210 nm，柱温为40 ℃，进样量为5 μL，流速为1.0 mL·min-1。建立复方鱼腥草合剂的指纹图谱，并通过对照品和液相色谱-质谱法（LC-MS）对共有峰进行指认和归属。检测3个厂家15批样品，并采用“中药色谱指纹图谱相似度评价系统”软件进行分析。结果：建立了复方鱼腥草合剂的HPLC指纹图谱，标定了16个共有峰，15批样品相似度均不低于0.95。结论：该方法样品处理简便，色谱条件简单、结果准确、重复性好，可以作为复方鱼腥草合剂质量控制的方法之一。 |
| 中文关键词:复方鱼腥草合剂 指纹图谱 高效液相色谱 黄芩苷 |
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| Fingerprints Analysis of Compound Yuxingcao Mixture by HPLC |
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| Abstract:Objective：To establish an HPLC for fingerprints analysis of compound Yuxingcao Mixture for its overall high accuracy quality control. Methods：The HPLC analysis was performed on Besil 5 C18-B （250 mm×4.6 mm，5 μm） with the mobile phase of methanol-0.1% phosphoric acid with gradient elution at the flow rate of 1.0 mL·min-1. The detection wavelength was set at 210 nm，the column temperature was at 40 ℃，and the injection volume was 5 μL. The common peaks were identified and attributed by combining the reference substance with LC-MS. 15 batches of samples which were made by three different manufacturers were determined and analyzed by Similarity Evaluation System for chromatographic Fingerprint of TCM. Results：The HPLC fingerprint of compound Yuxingcao Mixture was established. Totally 16 common characteristic peaks were identified. And the similarity of HPLC fingerprints was above 0.95 for all samples. Conclusion：The method is simple and repeatable，which can be used for the quality control of compound Yuxingcao Mixture. |
| keywords:compound Yuxingcao Mixture fingerprints HPLC baicalin |
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