Comparative Study on the Inhibitory Effect of Angelicae Dahuricae Radix,Atractylodis Macrocephalae Rhizoma and Typhonii Rhizoma Extract on Tyrosinase
Abstract:Objective:To compare the inhibition of tyrosinase by water extract and alcohol extract of Angelicae Dahuricae Radix,Atractylodes Macrocephalae Rhizoma and Typhonii Rhizoma. Methods:Their human skin whitening activity was assessed by tyrosinase inhibition test. The inhibitory effect of Chinese medicine extract on the production of dopoquinone was evaluated by measuring the effect of dopoquinone before and after adding Chinese medicine extract. Results:This method was used to compare the inhibitory effects of three Chinese medicine water extracts and alcohol extracts on tyrosinae. Among them,the inhibitory effect of Angelicae Dahuricae Radix extract on tyrosinase was higher than that of Atractylodis Macrocephalae Rhizoma and Typhonii Rhizoma. The inhibitory rate of alcohol extract of Angelicae Dahuricae Radix on tyrosinase was 30.77% and the inhibitory rate of water extract of Angelicae Dahuricae Radix on tyrosinase was 20.40%. The inhibition rate of alcohol extract of Atractylodis Macrocephalae Rhizoma on tyrosinase was 14.71% and the inhibition rate of water extract of Atractylodis Macrocephalae Rhizoma on tyrosinase was 8.69%. The inhibition rate of alcohol extract of Typhonii Rhizoma on tyrosinase was 8.36% and the inhibition rate of water extract of Typhonii Rhizoma on tyrosinase was 3.34%. Conclusion:The study provides a reference for revealing the material basis of whitening active ingredients of traditional Chinese medicine extracts.