基于差向异构体分离的桃仁配方颗粒中苦杏仁苷含量测定
投稿时间:2019-11-05     点此下载全文
引用本文:霍文杰,丁青,刘晓霞,孙冬梅,黄敏烨,王利伟.基于差向异构体分离的桃仁配方颗粒中苦杏仁苷含量测定[J].中国现代中药,2020,22(12):2090-2093
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作者中文名作者英文名单位中文名单位英文名E-Mail
霍文杰 HUO Wen-jie 广东一方制药有限公司,广东佛山528244 Guangdong Yifang Pharmaceutical Co.,Ltd.,Foshan 528244,China  
丁青 DING Qing 广东一方制药有限公司,广东佛山528244 Guangdong Yifang Pharmaceutical Co.,Ltd.,Foshan 528244,China  
刘晓霞 LIU Xiao-xia 广东一方制药有限公司,广东佛山528244 Guangdong Yifang Pharmaceutical Co.,Ltd.,Foshan 528244,China  
孙冬梅 SUN Dong-mei 广东一方制药有限公司,广东佛山528244 Guangdong Yifang Pharmaceutical Co.,Ltd.,Foshan 528244,China 孙冬梅,主任中药师,研究方向:中药新药研究及中药质量评价;E-mail:1083289014@qq.com 
黄敏烨 HUANG Min-ye 广东一方制药有限公司,广东佛山528244 Guangdong Yifang Pharmaceutical Co.,Ltd.,Foshan 528244,China  
王利伟 WANG Li-wei 广东一方制药有限公司,广东佛山528244 Guangdong Yifang Pharmaceutical Co.,Ltd.,Foshan 528244,China  
基金项目:广东省科技计划项目(2018B030323004);2017佛山市重大科技项目(2016AG100912)
中文摘要:目的:采用超高效液相色谱串联二极管阵列检测器法(UPLC-DAD)对桃仁配方颗粒中苦杏仁苷的2种差向异构体(D型与L型)分离后进行测定。方法:以Waters ACQUITY UPLC HSS T3(100 mm×2.1 mm,1.8 μm)为色谱柱,以乙腈为流动相A,以0.1%磷酸溶液为流动相B,梯度洗脱,流速为0.4 mL·min-1;检测波长为210 nm;进样体积为1 μL;柱温为20 ℃。结果:D-苦杏仁苷与L-苦杏仁苷能有效分离,D-苦杏仁苷在质量浓度为4.08~407.92 μg·mL-1,线性回归方程为Y=0.037 8X-0.012 3,r=0.999 8;准确度为96.09%~104.51%。结论:本方法能准确测定桃仁配方颗粒中D-苦杏仁苷的含量。
中文关键词:桃仁配方颗粒  异构体  D-苦杏仁苷  含量测定
 
Determination of Amygdalin in Taoren Formula Granules Based on Differential Isomer Separation
Abstract:Objective:To determine two epimers (D and L) of amygdalin in Taoren formula granules by UPLC-DAD. Methods:Waters ACQUITY UPLC HSS T3 (100 mm×2.1 mm,1.8 μm) was used as chromatographic column,acetonitrile as mobile phase A,0.1% phosphoric acid solution as mobile phase B,gradient elution,flow rate was 0.4 mL·min-1,detection wavelength was 210 nm,injection volume was 1 μL,column temperature was 20 ℃. Results:D-amygdalin and L-amygdalin could be separated effectively. The linear regression equation was Y=0.037 8X-0.012 3,r=0.999 8,in the range of concentration of amygdalin from 4.08-407.92 μg·mL-1,and the accuracy was 96.09%-104.51%. Conclusion:The method can accurately determine the content of D-amygdalin in Taoren formula granules.
keywords:Taoren formula granules  isomer  D-amygdalin  determination of content
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