| 基于差向异构体分离的桃仁配方颗粒中苦杏仁苷含量测定 |
| 投稿时间：2019-11-05 点此下载全文
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| 引用本文：霍文杰,丁青,刘晓霞,孙冬梅,黄敏烨,王利伟.基于差向异构体分离的桃仁配方颗粒中苦杏仁苷含量测定[J].中国现代中药,2020,22(12):2090-2093 |
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| 基金项目:广东省科技计划项目（2018B030323004）；2017佛山市重大科技项目（2016AG100912） |
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| 中文摘要:目的：采用超高效液相色谱串联二极管阵列检测器法（UPLC-DAD）对桃仁配方颗粒中苦杏仁苷的2种差向异构体（D型与L型）分离后进行测定。方法：以Waters ACQUITY UPLC HSS T3（100 mm×2.1 mm，1.8 μm）为色谱柱，以乙腈为流动相A，以0.1%磷酸溶液为流动相B，梯度洗脱，流速为0.4 mL·min-1；检测波长为210 nm；进样体积为1 μL；柱温为20 ℃。结果：D-苦杏仁苷与L-苦杏仁苷能有效分离，D-苦杏仁苷在质量浓度为4.08~407.92 μg·mL-1，线性回归方程为Y=0.037 8X-0.012 3，r=0.999 8；准确度为96.09%~104.51%。结论：本方法能准确测定桃仁配方颗粒中D-苦杏仁苷的含量。 |
| 中文关键词:桃仁配方颗粒 异构体 D-苦杏仁苷 含量测定 |
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| Determination of Amygdalin in Taoren Formula Granules Based on Differential Isomer Separation |
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| Abstract:Objective：To determine two epimers （D and L） of amygdalin in Taoren formula granules by UPLC-DAD. Methods：Waters ACQUITY UPLC HSS T3 （100 mm×2.1 mm，1.8 μm） was used as chromatographic column，acetonitrile as mobile phase A，0.1% phosphoric acid solution as mobile phase B，gradient elution，flow rate was 0.4 mL·min-1，detection wavelength was 210 nm，injection volume was 1 μL，column temperature was 20 ℃. Results：D-amygdalin and L-amygdalin could be separated effectively. The linear regression equation was Y=0.037 8X-0.012 3，r=0.999 8，in the range of concentration of amygdalin from 4.08-407.92 μg·mL-1，and the accuracy was 96.09%-104.51%. Conclusion：The method can accurately determine the content of D-amygdalin in Taoren formula granules. |
| keywords:Taoren formula granules isomer D-amygdalin determination of content |
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