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作者中文名	作者英文名	单位中文名	单位英文名	E-Mail
甘雨	GAN Yu	中国中医科学院中药研究所中药鉴定与安全性评估北京市重点实验室，北京100700	Key Laboratory of Beijing for Identification and Safety Evaluation of Chinese Medicine，Institute of Chinese Materia Medica，China Academy of Chinese Medical Sciences，Beijing 100700，China
吴端	WU Duan	中国中医科学院中药研究所中药鉴定与安全性评估北京市重点实验室，北京100700	Key Laboratory of Beijing for Identification and Safety Evaluation of Chinese Medicine，Institute of Chinese Materia Medica，China Academy of Chinese Medical Sciences，Beijing 100700，China
张栋	ZHANG Dong	中国中医科学院中药研究所中药鉴定与安全性评估北京市重点实验室，北京100700	Key Laboratory of Beijing for Identification and Safety Evaluation of Chinese Medicine，Institute of Chinese Materia Medica，China Academy of Chinese Medical Sciences，Beijing 100700，China
王星文	WANG Xing-wen	中国中医科学院中药研究所中药鉴定与安全性评估北京市重点实验室，北京100700	Key Laboratory of Beijing for Identification and Safety Evaluation of Chinese Medicine，Institute of Chinese Materia Medica，China Academy of Chinese Medical Sciences，Beijing 100700，China
马婷玉	MA Ting-yu	中国中医科学院中药研究所中药鉴定与安全性评估北京市重点实验室，北京100700	Key Laboratory of Beijing for Identification and Safety Evaluation of Chinese Medicine，Institute of Chinese Materia Medica，China Academy of Chinese Medical Sciences，Beijing 100700，China
向丽	XIANG Li	中国中医科学院中药研究所中药鉴定与安全性评估北京市重点实验室，北京100700	Key Laboratory of Beijing for Identification and Safety Evaluation of Chinese Medicine，Institute of Chinese Materia Medica，China Academy of Chinese Medical Sciences，Beijing 100700，China	向丽，副研究员，研究方向：中药资源；E-mail：lxiang@icmm.ac.cn
王辉	WANG Hui	北京城市学院，北京100083	Beijing City University，Beijing 100083，China	王辉，副教授，研究方向：药理学；E-mail：wanghui2015@bcu.edu.cn

基金项目:国家“重大新药创制”科技重大专项（2017ZX09101002-003-001，2019ZX09201005-006-001）；国家自然科学基金项目（U1812403-1）；北京城市学院2019年度实培计划项目；中央级公益性科研院所基本科研业务费专项（ZZ10-024）

中文摘要:目的：基于黄花蒿全基因组及转录组数据，进行AabHLH基因家族生物信息学分析及在不同光处理下的基因表达分析。方法：基于基因隐马科夫模型（HMM）对AabHLH基因进行鉴定，利用Pfam、GSDS、MEME等在线分析软件对基因结构、分子进化及保守结构域等进行分析。结果：全基因组水平共鉴定出99条黄花蒿AabHLH基因，包含49对复制基因对；基于系统发育树将其分为13个亚族，所有亚族AabHLH蛋白亚细胞定位于细胞核且均为亲水性蛋白，同一亚族基因具有相似的外显子-内含子结构及保守基序。基因表达模式分析发现，AabHLH基因在不同水平上响应蓝光、红光、远红光、白光调控，推测其中6个亚族基因为光照条件下，7个亚族基因为非光照条件下青蒿素合成途径调控基因。结论：在全基因组水平鉴定了AabHLH基因，为深度解析AabHLH基因功能及其对光调控下青蒿素生物合成调控机制提供参考。

中文关键词:黄花蒿 bHLH转录因子光调控表达分析

Identification and Light Regulation Analysis of bHLH Transcription Factor Gene Family in Artemisia annua

Abstract:Objective：Based on the whole-genome and transcriptome data，the bioinformatics analysis of bHLH gene family in Artemisia annua and gene expression analysis under different light treatments were performed in this study. Methods：AabHLH genes are identified based on the genetic hidden Markov model （HMM），and their gene structures，molecular evolution and conserved domains are analyzed using online analysis software such as Pfam，GSDS，MEME，etc. Results：99 AabHLH genes were identified at the genome-wide level，containing a total of 49 replicated gene pairs. All the AabHLH proteins are hydrophilic proteins and located in the nucleus. They are divided into 13 subfamilies based on phylogenetic tree and the same subfamily genes have similar exon-intron structures and conserved motifs. Gene expression analysis found that AabHLH genes responded to blue light，red light，far red light，and white light at different levels. It is speculated that 6 subfamily genes of AabHLH are key regulatory genes for artemisinin synthesis under light regulation，while 7 subfamily genes are non-light regulation genes. Conclusion：This study identified AabHLH genes in all genome levels，and provides some supports for further researches on the function of the AabHLH genes and their regulation of artemisinin biosynthesis under light.

keywords:Artemisia annua L. bHLH transcription factor light regulation expression analysis

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