须根水提液对白花丹参种子萌发幼苗光合作用及次生代谢的影响
投稿时间:2019-09-29     点此下载全文
引用本文:史国玉,周冰谦,刘伟,王晓,耿岩玲,张琳,赵珂,李奉胜.须根水提液对白花丹参种子萌发幼苗光合作用及次生代谢的影响[J].中国现代中药,2021,23(3):491-495
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作者中文名作者英文名单位中文名单位英文名E-Mail
史国玉 SHI Guo-yu 山东医学高等专科学校,山东济南250002 Shandong Medical College
Jinan 250002,China
 
周冰谦 ZHOU Bing-qian 齐鲁工业大学/山东省科学院 山东省分析测试中心,山东济南250014 Qilu University of Technology/Shandong Analysis and Test Center,Shandong Academy of Sciences,Jinan 250014,China  
刘伟 LIU Wei 齐鲁工业大学/山东省科学院 山东省分析测试中心,山东济南250014 Qilu University of Technology/Shandong Analysis and Test Center,Shandong Academy of Sciences,Jinan 250014,China 刘伟 ,研究员,研究方向:中药资源与质量控制;Tel:(0531)68606191,E-mail:liuwei0074@163.com 
王晓 WANG Xiao 齐鲁工业大学/山东省科学院 山东省分析测试中心,山东济南250014 Qilu University of Technology/Shandong Analysis and Test Center,Shandong Academy of Sciences,Jinan 250014,China  
耿岩玲 GENG Yan-ling 齐鲁工业大学/山东省科学院 山东省分析测试中心,山东济南250014 Qilu University of Technology/Shandong Analysis and Test Center,Shandong Academy of Sciences,Jinan 250014,China  
张琳 ZHANG Lin 山东第一医科大学 第二附属医院,山东泰安271000 The Second Affiliated Hospital of Shandong First Medical University,Tai′an 271000,China  
赵珂 ZHAO Ke 莱芜紫光生态园有限公司,山东济南271100 Laiwu Ziguang Ecological Co.,Ltd.,Jinan 271100,China  
李奉胜 LI Feng-sheng 莱芜紫光生态园有限公司,山东济南271100 Laiwu Ziguang Ecological Co.,Ltd.,Jinan 271100,China  
基金项目:中央本级重大增减支项目(2060302);山东省高等学校科技计划项目(J17KA261);山东省中医药科技发展计划项目(2019-0314);山东省重点研发计划项目(2017CXGC1304)
中文摘要:目的:研究不同浓度白花丹参须根水提液对种子萌发、幼苗光合作用及次生代谢物质积累的影响,以期为白花丹参连作障碍的消减提供参考。方法:设置不同浓度白花丹参须根水提液处理组,测定不同处理组种子萌发率、幼苗多酚氧化酶活性、可溶性糖及可溶性蛋白含量;利用高效液相色谱法(HPLC)测定幼苗中水溶性及脂溶性有效物质含量。结果:白花丹参须根水提液低浓度对种子萌发有促进作用,高浓度有抑制作用;与对照组相比,处理组幼苗净光合速率、蒸腾速率、气孔导度均有不同程度下降;对照组多酚氧化酶活性及可溶性蛋白、可溶性糖总量分别比白花丹参须根水提液处理后低7.86%、8.47%、14.33%,但处理后幼苗水溶性有效成分(丹酚酸B、迷迭香酸)及脂溶性有效成分(二氢丹参酮、丹参酮Ⅰ、丹参酮ⅡA、隐丹参酮)含量积累量均高于对照组。结论:白花丹参须根水提液通过影响种子的萌发率、抑制幼苗的光合作用、促进次生代谢产物积累影响丹参的产量及品质。
中文关键词:白花丹参  水提液  种子萌发  生理活性  次生代谢
 
Effects of Water Extract of Fibrous Root on Seed Germination,Photosynthesis and Secondary Metabolism of Salvia miltiorrhiza
Abstract:Objective:In order to provide basic data for reducing the continuous cropping obstacle of Salvia miltiorrhiza Bge. F. alba,the effects of different concentrations of fibrous root water extract on seed germination,seedling photosynthesis and secondary metabolite accumulation were studied. Methods:Germination rate of seeds,polyphenol oxidase activity,soluble sugar and protein content were measured in different treatment groups,and also the content of water-soluble and lipid soluble effective substances in S.miltiorrhiza seedlings were determined by HPLC. Results:Compared with the control group,the net photosynthetic rate (Pn),transpiration rate (Tr) and stomatal conductance (Gs) of the treated group are decreased. The PPO activity,the content of soluble protein and total soluble sugar in the control group were 7.86%,8.47% and 14.33% lower than those in the fibrous root water extract group,respectively,but the accumulation of water-soluble (salvianolic acid B,rosmarinic acid) and lipid soluble effective substance (dihydrotanshinone,tanshinone Ⅰ,tanshinone ⅡA,cryptotanshinone) in the treated seedlings were higher than that in the control group. Conclusion:The water extract of fibrous root affected the germination rate of seeds,inhibited photosynthesis of seedlings,promoted the accumulation of secondary metabolites,and affected the yield and quality of S. miltiorrhiza.
keywords:Salvia miltiorrhiza Bge.F.alba  water extract  seed germination  physiological activity  secondary metabolism
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