| 不同煎煮工艺对四君子汤有效成分和免疫调节功能的影响 |
| 投稿时间：2020-04-14 点此下载全文
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| 引用本文：吕邵娃,郭乔馨,尚尔雨,吴茜,于新,郭玉岩,杨大宇,李永吉,孙爽.不同煎煮工艺对四君子汤有效成分和免疫调节功能的影响[J].中国现代中药,2021,23(3):518-523 |
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| 基金项目:国家自然科学基金项目（81803985）；黑龙江省自然科学基金项目（H2016060） |
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| 中文摘要:目的：研究不同煎煮工艺对四君子汤有效成分和胃肠道免疫调节功能的影响。方法：前期由单因素试验结果得出各因素最佳参数范围，在确定煎煮2次的基础上，选浸泡时间（A）、煎煮时间（B）、加水量（C）为因素，每个因素选3个水平，得到四君子汤9种煎煮工艺；采用高效液相色谱法（HPLC）检测人参皂苷Rg1、人参皂苷Re、甘草酸含量；采用水溶性浸出物测定法测定浸出率；采用流式细胞术检测四君子汤对脾虚大鼠肠黏膜CD3阳性细胞占比的影响。采用L9（34）正交试验法，根据化学指标和药理活性指标优选出最佳煎煮工艺。结果：当浸泡时间为2 h、加水量为8倍、提取2次、每次40 min时，四君子汤提取物中人参皂苷Rg1、人参皂苷Re、甘草酸含量最高，调节胃肠道免疫的活性更好。结论：优化了四君子汤煎煮工艺，为四君子汤的古方今用及后续研究提供参考。 |
| 中文关键词:四君子汤 正交试验 煎煮工艺 细胞免疫 药理活性 |
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| Effect of Different Decoction Methods of Sijunzi Decoction on Effective Components and Immunomodulatory Function |
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| Abstract:Objective：To study the effect of different decoction methods of Sijunzi Decoction on the effective components and gastrointestinal immune function.Method：In the early stage，the optimal parameter range of each factor can be obtained from the results of single factor experiment. On the basis of determining the frying for 2 times，in this experiment，immersion time （A），decoction time （B） and water content （C） were selected as the factors，and three levels were selected for each factor. Orthogonal design method was used to detect the content and the ratio of extraction of ginsenoside Rg1，ginsenoside Re and glycyrrhizic acid in Sijunzi Decoction. The leaching rate was determined by the method of water-soluble extract. According to the percentage of CD3 cells in intestinal mucosa of rats with spleen deficiency and the effect of Sijunzi Decoction on CD3 cells，the pharmacodynamic evaluation of different decoction methods was investigated，and to design the best decoction method according to the results. Results：When the soaking time is 2 h，add 8 times of water and extract twice for 40 min，the content of effective ingredients is higher and the gastrointestinal immune activity is better. Conclusion：In summary，the optimized decoction methods is stable，economical，convenient and efficient，which lays a foundation for the subsequent research on Sijunzi Decoction. |
| keywords:Sijunzi Decoction orthogonal design decoction methods cellular immune pharmacological activity |
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