| 大别山红天麻饮片多指标质量标志物研究 |
| 投稿时间：2020-06-01 点此下载全文
|
| 引用本文：耿文洁,汪聪聪,刘劲松,桂双英,彭代银,王举涛.大别山红天麻饮片多指标质量标志物研究[J].中国现代中药,2021,23(3):524-528 |
| DOI： |
| 摘要点击次数: 528 |
| 全文下载次数: 185 |
|
|
| 基金项目:安徽省高等学校自然科学研究基金重点项目（KJ2019A0462）；安徽省中央引导地方科技发展专项（YDZX20183400004233）；安徽省科技重大专项（18030801128） |
|
| 中文摘要:目的：对大别山红天麻饮片质量标志物进行研究，多指标成分评价测定其中9种质量标志物，推进红天麻质量标志物的相关研究进展。方法：采用高效液相色谱法（HPLC），以天麻素为内参物（ZS），建立该成分与腺苷、对羟基苯甲醇、对羟基苯甲醛、对羟基苯甲酸、巴利森苷A、巴利森苷B、巴利森苷 C、巴利森苷 E的相对校正因子，采用相对校正因子计算各成分的质量分数，建立大别山产红天麻饮片的质量标准。结果：红天麻饮片质量标志物的9种成分均达到基线分离，线性关系均良好（r≥0.999 5）；平均加样回收率为 96.86％～102.21％。相对校正因子的RSD均小于2%。并指认出其指纹图谱中10种化学成分，建立大别山红天麻特征图谱。结论：一测多评（QAMS）简便、准确、专属性强，选择的质量标志物科学合理，可用于大别山红天麻饮片多指标质量标志物的同步测定，建立的含量测定方法可为红天麻的质量评价及控制提供参考。 |
| 中文关键词:大别山红天麻饮片 中药质量标志物 一测多评 质量标准 |
| |
| Study on Multi-indicator Quality Markers of Red Gastrodia Decoction Pieces in Ta-pieh Mountain |
|
|
| Abstract:Objective：To study the quality markers of red Gastrodia decoction pieces in Ta-pieh Mountain，evaluate and determine 9 quality markers in multi-index components，and promote the research progress of quality markers of Red G.elata. Methods：The HPLC method was used，with gastrodin as the internal reference material （ZS），and this component was established with adenosine，p-hydroxybenzyl alcohol，p-hydroxybenzaldehyde，p-hydroxybenzoic acid，parison A，parison B，parison C and parison E. The relative correction factors were used to calculate the mass fraction of each component，and the quality standards for the red G.elata decoction pieces produced in the Ta-pieh Mountain area were established. Results：9 components of the quality markers of Red G.elata decoction pieces all reached baseline separation，and the linear relationship was good （r≥0.999 5）. The average recovery rate was 96.86% to 102.21%. The RSD values of relative correction factors were all <2%. And identified 10 kinds of chemical components in its fingerprint，and established a characteristic map of G.elata in Ta-pieh Mountain. Conclusion：The one-test multi-evaluation method is simple，accurate，and highly specific，the selected quality markers are scientific and reasonable. It can be used for the simultaneous determination of multi-index quality markers of Ta-piehshan Red G.elata decoction pieces. The established content determination method provides a scientific basis for the quality evaluation and control of Red G.elata. |
| keywords:Red Gastrodia decoction pieces in Ta-pieh Mountain quality markers of traditional Chinese medicine QAMS quality standard |
|
查看全文
查看/发表评论 下载PDF阅读器 |
