基于HPLC-Q/TOF-MS的经典名方苓桂术甘汤成分快速分析 |
投稿时间:2020-01-14 点此下载全文
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引用本文:闵会,罗婷婷,印晓红,徐斌,查慧军,王建方,王如伟.基于HPLC-Q/TOF-MS的经典名方苓桂术甘汤成分快速分析[J].中国现代中药,2021,23(3):529-535 |
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基金项目:重大科技专项重点社会发展项目(2013C03005) |
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中文摘要:目的:采用高效液相色谱-四级杆/飞行时间串联质谱法(HPLC-Q/TOF-MS)建立苓桂术甘汤成分快速分析方法,实现对苓桂术甘汤物质基础的表征。方法:采用Welch Ultimate XB-C18色谱柱(250 mm×4.6 mm,5 μm),以流动相乙腈(B)-0.1%甲酸水(A)溶液进行梯度洗脱,流速为0.8 mL·min-1,柱温为25 ℃,进样量为10 μL。质谱采用电喷雾离子源(ESI)正、负离子模式,扫描范围mz 50~1000。结果:对正负模式下的质谱信息进行分析,并结合相关文献,共鉴定出苓桂术甘汤中60个化学成分,其中包括有机酸类成分5个、黄酮类成分17个、三萜类成分24个、内酯类成分5个,另有氨基酸类、核苷酸类、糖苷类等成分。结论:HPLC-Q/TOF-MS技术能实现对苓桂术甘汤的全成分快速分析、鉴定。 |
中文关键词:经典名方 苓桂术甘汤 高效液相色谱-四级杆/飞行时间串联质谱 快速分析 |
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Rapid Analysis Components of Lingguizhugan Decoction with HPLC-Q/TOF-MS |
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Abstract:Objective:To establish a rapid analysis method by HPLC-Q/TOF-MS,and acquire the material basis characterization of Lingguizhugan Decoction. Methods:The analysis was performed on a Welch Ultimate XB-C18 (250 mm×4.6 mm,5 μm) HPLC column. The mobile phase consisted of acetonitrile (B) and 0.1% formic acid (A) was used as gradient elute. The flow rate was 0.8 mL· min-1,column temperature was 25 ℃,and injection volume was 10 μL. The ESI was operated in positive mode and negative mode,and scanning range was mz 50-1000. Results:Through the analysis of mass spectrometry data in positive and negative modes and with the help of relevant literature,a total of 60 chemical compounds in Lingguizhugan Decoction were identified,including 5 organic acid compounds,17 flavonoid compounds,24 triterpenoid compounds,5 lactone compounds,besides,amino acids,nucleotides,and glycosides were also identified. Conclusion:Via the HPLC-Q/TOF-MS technology,it was achieved to rapidly analyze and identify the whole compounds of Lingguizhugan Decoction. |
keywords:classical prescription Lingguizhugan Decoction HPLC-Q/TOF-MS rapid analysis |
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