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作者中文名	作者英文名	单位中文名	单位英文名	E-Mail
范恺磊	FAN Kai-lei	江阴天江药业有限公司，江苏江阴214434	Jiangyin Tianjiang Pharmaceutical Co.，Ltd.，Jiangyin 214434，China
王协和	WANG Xie-he	江阴天江药业有限公司，江苏江阴214434	Jiangyin Tianjiang Pharmaceutical Co.，Ltd.，Jiangyin 214434，China
杜俊潮	DU Jun-chao	江阴天江药业有限公司，江苏江阴214434	Jiangyin Tianjiang Pharmaceutical Co.，Ltd.，Jiangyin 214434，China
李松	LI Song	江阴天江药业有限公司，江苏江阴214434	Jiangyin Tianjiang Pharmaceutical Co.，Ltd.，Jiangyin 214434，China
张云天	ZHANG Yun-tian	江阴天江药业有限公司，江苏江阴214434	Jiangyin Tianjiang Pharmaceutical Co.，Ltd.，Jiangyin 214434，China
陈盛君	CHEN Sheng-jun	江阴天江药业有限公司，江苏江阴214434	Jiangyin Tianjiang Pharmaceutical Co.，Ltd.，Jiangyin 214434，China	陈盛君，副主任药师，研究方向：中药研发；Tel：（0510）80631032，E-mail：chensjxz1123@163.com
陈可冀	CHEN Ke-ji	福建中医药大学中西医结合研究院，福建福州350112	Academy of Integrative Medicine，Fujian University of Traditional Chinese Medicine，Fuzhou 350122，China
彭军	PENG Jun	福建中医药大学中西医结合研究院，福建福州350112	Academy of Integrative Medicine，Fujian University of Traditional Chinese Medicine，Fuzhou 350122，China
许运明	XU Yun-ming	江阴天江药业有限公司，江苏江阴214434	Jiangyin Tianjiang Pharmaceutical Co.，Ltd.，Jiangyin 214434，China

基金项目:国家“重大新药创制”科技重大专项（2018ZX09201011-004-004）

中文摘要:目的：建立超高效液相色谱法（UPLC）一测多评（QAMS）同时测定钩藤饮片中钩藤碱、异钩藤碱、去氢钩藤碱、异去氢钩藤碱4种化学成分含量的方法，并验证该方法的可行性和准确性。方法：以钩藤饮片的4种成分为指标成分，以异钩藤碱为内标，分别计算得到钩藤碱、异去氢钩藤碱与去氢钩藤碱的相对较正因子，实现QAMS同时测定钩藤饮片中4种生物碱类成分含量。结果：在线性范围内，钩藤碱、异钩藤碱、去氢钩藤碱、异去氢钩藤碱的相对校正因子（RCF）分别为1.02、0.97、1.00、1.01，在不同实验条件下RCF的系统耐用性良好。结论：建立的以异钩藤碱为内标，同时测定4种钩藤生物碱的一测多评方法稳定、准确度高、适应性好，可用于钩藤饮片中4种生物碱类化学成分的含量测定。

中文关键词:钩藤一测多评校正因子钩藤碱异钩藤碱去氢钩藤碱异去氢钩藤碱

Simultaneously Quantitative Analysis of Four Alkaloids of Ramulus Uncariae cum Uncis Based on Multi-components with Single Marker

Abstract:Objective：To establish a UPLC method for simultaneous determination of the contents of four components （rhynchophylline，isorhynchophylline，isomitraphylline，isocorynoxeine） in decoction pieces of Ramulus Uncariae cum Uncis by quantitatively analyzing multi-components with single-marker （QAMS），and verify the feasibility and technical adaptability of this method. Methods：Four alkaloids in Ramulus Uncariae cum Unciswere used as index ingredients. With isorhynchophylline as the internal standard，the relative correction factors （RCF） of rhynchophylline，isomitraphylline，and isocorynoxeine were established respectively，and the quantities of four components in the decoction pieces of Ramulus Uncariae cum Uncis were calculated. Results：In the linear range，the relative correction factors of rhynchophylline，isorhynchophylline，isomitraphylline，and isocorynoxeine were 1.02，0.97，1.00 and 1.01，respectively. The relative correction factors had good reproducibility under different experimental conditions. Conclusion：The method of simultaneous determination of rhynchophylline，isorhynchophylline，isomitraphylline，and isocorynoxeine with isorhynchophylline as internal standard is scientifically stable，accurate and adaptable，and can be used for the determination of 4 alkaloids in Ramulus Uncariae cum Uncis.

keywords:Ramulus Uncariae cum Uncis QAMS correction factor rhynchophylline isorhynchophylline isocorynoxeine corynoxeine

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