| 半夏白术天麻汤治疗癫痫作用机制的网络药理学研究 |
| 投稿时间：2020-08-07 点此下载全文
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| 引用本文：卢玲,胡跃强,李欢,廖现秋,何乾超,蔡伦,刁丽梅.半夏白术天麻汤治疗癫痫作用机制的网络药理学研究[J].中国现代中药,2021,23(8):1406-1415 |
| DOI：10.13313/j.issn.1673-4890.20200807003 |
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| 作者中文名 | 作者英文名 | 单位中文名 | 单位英文名 | E-Mail |
| 卢玲 |
LU Ling |
广西中医药大学 研究生院，广西 南宁 530001 |
Graduate School of Guangxi University of Chinese Medicine, Nanning 530001, China |
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| 胡跃强 |
HU Yue-qiang |
广西中医药大学 第一附属医院，广西 南宁 530023 |
The First Affiliated Hospital of Guangxi University of Chinese Medicine, Nanning 530023, China |
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| 李欢 |
LI Huan |
广西中医药大学 研究生院，广西 南宁 530001 |
Graduate School of Guangxi University of Chinese Medicine, Nanning 530001, China |
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| 廖现秋 |
LIAO Xian-qiu |
广西中医药大学 研究生院，广西 南宁 530001 |
Graduate School of Guangxi University of Chinese Medicine, Nanning 530001, China |
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| 何乾超 |
HE Qian-chao |
广西中医药大学 第一附属医院，广西 南宁 530023 |
The First Affiliated Hospital of Guangxi University of Chinese Medicine, Nanning 530023, China |
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| 蔡伦 |
CAI Lun |
广西中医药大学 第一附属医院，广西 南宁 530023 |
The First Affiliated Hospital of Guangxi University of Chinese Medicine, Nanning 530023, China |
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| 刁丽梅 |
DIAO Li-mei |
广西中医药大学 第一附属医院，广西 南宁 530023 |
The First Affiliated Hospital of Guangxi University of Chinese Medicine, Nanning 530023, China |
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| 基金项目:国家自然科学基金项目（81760809，81960858）；广西自然科学基金项目（2017GXNSFAA198294）；广西中医药大学岐黄工程高层次人才团队培育项目（2018003）；广西医疗卫生适宜技术开发与推广应用项目（s2017049）；广西中医药大学2019年研究生教育创新计划项目（YCSY20190062） |
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| 中文摘要:目的 运用网络药理学技术探讨半夏白术天麻汤治疗癫痫的作用机制。方法 分别在中药系统药理学数据库与分析平台（TCMSP）、BATMAN-TCM数据库及TCMID数据库中检索并整合得到半夏白术天麻汤的活性成分及活性成分作用的靶点。在OMIM数据库和GeneCards数据库中检索并整合得到癫痫的相关靶点。通过Venny软件映射得到半夏白术天麻汤治疗癫痫的潜在靶点。通过Cytoscape 3.6.1软件构建成分-靶点关系网络，通过STRING 11.5数据库构建蛋白质-蛋白质相互作用网络，筛选核心治疗靶点。基于R语言软件对半夏白术天麻汤治疗癫痫的潜在靶点进行基因本体（GO）富集分析、京都基因与基因组百科全书（KEGG）通路富集分析，并且构建KEGG关系网络。结果 共筛选出半夏白术天麻汤活性成分130个，半夏白术天麻汤治疗癫痫的潜在靶点258个，其中核心靶点5个，包括β2肾上腺素受体（ADRB2）、钙调蛋白1（CALM1）、雌激素受体1（ESR1）、一氧化氮合酶2（NOS2）、丝裂原活化蛋白激酶14（MAPK14）。且靶点之间具有直接或间接的相互作用关系。其涉及的GO生物学主要包括γ-氨基丁酸（GABA）能突触、神经递质受体的活动、GABA-A受体活动、GABA受体活动、苯二氮卓受体活动等。其主要涉及的KEGG通路包括白细胞介素-17（IL-17）信号通路、5-羟色胺能突触等。结论 半夏白术天麻汤通过多成分、多靶点、多途径对癫痫发挥作用。 |
| 中文关键词:癫痫 网络药理学 半夏白术天麻汤 靶点 通路 |
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| Mechanism of Banxia Baizhu Tianma Decoction in Treating Epilepsy: Based on Network Pharmacology |
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| Abstract:Objective To explore the mechanism of Banxia Baizhu Tianma Decoction (BBTD) in the treatment of epilepsy based on network pharmacology.Methods Firstly, the active components of BBTD were retrieved from Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform (TCMSP), Bioinformatics Analysis Tool of Molecular mechanism of Traditional Chinese Medicine (BATMAN-TCM), and Traditional Chinese Medicine Integrated Database (TCMID), targets of the active components from TCMSP and BATMAN-TCM, and targets related to epilepsy from OMIM and GeneCards. Then, the potential targets of BBTD in the treatment of epilepsy were mapped by Venny. The component-target network was constructed by Cytoscape 3.6.1 and protein-protein interaction (PPI) network by STRING 11.5 to screen the hub genes, followed by gene ontology (GO) term enrichment and kyoto encyclopedia of genes and genomes (KEGG) pathway enrichment for the common targets of the disease and the medicine based on R language. Then the potential target-pathway network was established.Results A total of 130 active components of BBTD and 258 potential targets related to the treatment of epilepsy were retrieved, including 5 hub genes:β2-adrenergic receptor (ADRB2), calmodulin 1 (CALM1), estrogen receptors alpha (ESR1), nitric oxide synthase 2 (NOS2), and mitogen-activated protein kinase 14 (MAPK14). The 258 targets showed direct or indirect interaction, which involved the GO terms of gamma-aminobutyric acid (GABA)ergic synapse, neurotransmitter receptor activity, gamma-aminobutyric acid A (GABA-A) receptor activity, GABA receptor activity, and benzodiazepine receptor activity, and the pathways of interleukin-17 (IL-17)signaling pathway and serotoninergic synapses.Conclusion BBTD acts on epilepsy through multiple components, targets, and pathways. |
| keywords:epilepsy network pharmacology Banxia Baizhu Tianma Decoction targets pathways |
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