| 山茱萸不同炮制品中8个化学成分的含量测定 |
| 投稿时间：2020-07-29 点此下载全文
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| 引用本文：肖红,王东春,舒琴,周莉娟.山茱萸不同炮制品中8个化学成分的含量测定[J].中国现代中药,2021,23(8):1444-1450 |
| DOI：10.13313/j.issn.1673-4890.20200729005 |
| 摘要点击次数: 413 |
| 全文下载次数: 144 |
| 作者中文名 | 作者英文名 | 单位中文名 | 单位英文名 | E-Mail |
| 肖红 |
XIAO Hong |
内江市第二人民医院 药剂科，四川 内江 641000 |
Pharmacy Department, Neijiang Second People's Hospital, Neijiang 641000, China |
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| 王东春 |
WANG Dong-chun |
内江市第二人民医院 药剂科，四川 内江 641000 |
Pharmacy Department, Neijiang Second People's Hospital, Neijiang 641000, China |
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| 舒琴 |
SHU Qin |
内江市第二人民医院 药剂科，四川 内江 641000 |
Pharmacy Department, Neijiang Second People's Hospital, Neijiang 641000, China |
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| 周莉娟 |
ZHOU Li-juan |
内江市第二人民医院 药剂科，四川 内江 641000 |
Pharmacy Department, Neijiang Second People's Hospital, Neijiang 641000, China |
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| 中文摘要:目的 建立高效液相色谱法（HPLC）测定不同方法炮制的山茱萸中没食子酸、5-羟甲基糠醛、莫诺苷、獐牙菜苷、马钱苷、山茱萸新苷Ⅰ、齐墩果酸和熊果酸的含量，阐述不同炮制方法对山茱萸中8个成分含量的影响。方法 采用HPLC，色谱柱为Stamsil C18（250 mm×4.6 mm，5 μm），流动相为乙腈-0.1%磷酸水溶液，梯度洗脱，流速为1.0 mL·min–1 ，检测波长分别为218、284、240、360 nm，柱温为30 ℃。结果 没食子酸、5-羟甲基糠醛、莫诺苷、獐牙菜苷、马钱苷、山茱萸新苷Ⅰ、齐墩果酸、熊果酸线性范围分别为0.80~32.07（r=0.999 4）、0.02~0.73（r=0.999 5）、26.91~1 076.27（r=0.999 4）、0.51~20.47（r=0.999 1）、12.47~498.80（r=0.999 7）、4.29~171.60（r=0.999 6）、6.59~263.67（r=0.999 5）、2.87~114.87 μg·mL–1（r=0.999 1）；精密度、稳定性、重复性试验RSD均小于3.0%；平均加样回收率为99.48%~104.32%（n=9），RSD均小于3.0%。与生品相比，山茱萸经炮制后熊果酸、没食子酸、齐墩果酸、5-羟甲基糠醛含量增加，马钱苷、莫诺苷、獐牙菜苷、山茱萸新苷Ⅰ含量减少。结论 该方法操作重复性好、准确、简便，可同时测定山茱萸不同炮制品中8个化学成分的含量。 |
| 中文关键词:山茱萸 高效液相色谱法 含量测定 炮制 |
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| Determination of Eight Major Components in Different Processed Products of Corni Fructus |
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| Abstract:Objective To establish a high performance liquid chromatography (HPLC) method for the content determination of gallic acid, 5-hydroxymethyl-2-furaldehyde, morroniside, sweroside, loganin, cornuside I, oleanolic acid, and ursolic acid in different processed products of Corni Fructus, and expound the effects of different processing methods on eight components in Corni Fructus.Methods The HPLC analysis was performed on a Stamsil C18 (250 mm × 4.6 mm, 5 μm) column. The mobile phase was a mixture of acetonitrile and 0.1% phosphoric acid for gradient elution. The flow rate and column temperature were set at 1.0 mL·min–1 and 30 ℃, respectively. The detection was conducted at the wavelengths of 218, 284, 240, 360 nm.Results The linear ranges for gallic acid, 5-hydroxymethyl-2-furaldehyde, morroniside, sweroside, loganin, cornuside I, oleanolic acid, and ursolic acid were 0.80-32.07 (r=0.999 4), 0.02-0.73 (r=0.999 5), 26.91-1 076.27 (r=0.999 4), 0.51-20.47 (r=0.999 1), 12.47-498.80 (r=0.999 7), 4.29-171.60 (r= 0.999 6), 6.59-263.67 (r= 0.999 5), 2.87-114.87 μg·mL–1 (r= 0.999 1), respectively. The RSD values of precision, stability, and reproducibility tests were all lower than 3.0%. The average recovery rates were within the range of 99.48%-104.32% (n=9), with the RSDs less than 3.0%. Compared with the raw Corni Fructus, the processed ones exhibited increased ursolic acid, gallic acid, oleanolic acid, and 5-hydroxymethyl-2-furaldehyde but decreased loganin, morroniside, sweroside and cornuside I.Conclusion The method is reproducible, accurate, and simple, which can be used for simultaneously determining the contents of eight chemical components in different processed products of Corni Fructus. |
| keywords:Corni Fructus HPLC content determination processing |
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