甘草地上部分寡糖富集纯化及其抗衰老活性研究 |
投稿时间:2021-01-27 点此下载全文
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引用本文:于冰莉,方永晟,庞昕昕,郭小趁,王文全,刘永刚.甘草地上部分寡糖富集纯化及其抗衰老活性研究[J].中国现代中药,2021,23(11):1889-1896 |
DOI:10.13313/j.issn.1673-4890.20210127005 |
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作者中文名 | 作者英文名 | 单位中文名 | 单位英文名 | E-Mail |
于冰莉 |
YU Bing-li |
北京中医药大学 中药学院,北京 102488 |
School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 102488, China |
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方永晟 |
FANG Yong-sheng |
北京中医药大学 中药学院,北京 102488 |
School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 102488, China |
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庞昕昕 |
PANG Xin-xin |
北京中医药大学 中药学院,北京 102488 |
School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 102488, China |
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郭小趁 |
GUO Xiao-chen |
北京中医药大学 中药学院,北京 102488 |
School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 102488, China |
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王文全* |
WANG Wen-quan |
北京中医药大学 中药学院,北京 102488 中国医学科学院 北京协和医学院 药用植物研究所,北京 100193 |
School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 102488, China Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100193, China |
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刘永刚 |
LIU Yong-gang |
北京中医药大学 中药学院,北京 102488 |
School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 102488, China |
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基金项目:国家重点研发计划(2018YFC1706505) |
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中文摘要:目的 制备甘草地上部分寡糖,对其进行表征及体内外抗衰老活性评价。方法 用水回流提取甘草地上部分,经过AB-8型大孔吸附树脂、DEAE-52纤维素、Sephadex LH-20树脂等富集纯化寡糖;通过凝胶渗透色谱法测定样品相对分子质量,薄层色谱法鉴别样品中是否含有葡萄糖和果糖,并通过1,1-二苯基-2-三硝基苯肼(DPPH)自由基清除实验评价其体外抗氧化能力;通过秀丽隐杆线虫氧化应激、热应激、寿命、脂褐素测定等实验评价其体内抗衰老活性。结果 富集纯化得到的甘草地上部分寡糖样品相对分子质量为180~1800,不含葡萄糖和果糖。样品对DPPH自由基有较好的清除作用,半数抑制浓度为0.220 2 mg·mL–1。甘草地上部分寡糖能显著延长氧化应激、热应激下线虫的寿命,减少线虫体内脂褐素的沉积。结论 甘草地上部分提取分离纯化得到的寡糖样品具有良好的体内外抗氧化、抗衰老活性。 |
中文关键词:甘草地上部分 寡糖 秀丽线虫 抗氧化活性 抗衰老活性 |
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Enrichment, Purification, and Anti-aging Activity Evaluation of Oligosaccharides in Aerial Parts of Glycyrrhiza uralensis |
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Abstract:Objective To extract oligosaccharides from the aerial parts of Glycyrrhiza uralensis Fisch., characterize them, and evaluate their anti-aging activity in vivo and in vitro.Methods The aerial parts of Glycyrrhiza uralensis Fisch. were extracted by water reflux, and then the oligosaccharides were enriched and purified by AB-8 macroporous adsorption resin, DEAE-52 cellulose, and Sephadex LH-20 resin. Gel permeation chromatography was adopted to determine the molecular weight of the sample, and thin-layer chromatography to differentiate whether the sample contained glucose and fructose. The in vitro antioxidant activity was evaluated based on the 1,1-diphenyl-2-picrylhydrazyl (DPPH)-scavenging ability and in vivo anti-aging activity by testing the survival of Caenorhabditis elegans under oxidative stress and heat stress and the lipofuscin content in the nematodes.Results The molecular weight of the yielded oligosaccharides was in the range of 180-1800, and they did not contain glucose or fructose. The oligosaccharide sample showed high DPPH-scavenging activity, with the half maximal inhibitory concentration (IC50) of 0.220 2 mg·mL–1. The sample can significantly prolong the life span of C. elegans under oxidative stress and heat stress and reduce the accumulation of lipofuscin in it.Conclusion The oligosaccharides from the aerial parts of G. uralensis have high in vitro antioxidant activity and in vivo anti-aging activity. |
keywords:aerial parts of Glycyrrhiza uralensis Fisch. oligosaccharide Caenorhabditis elegans antioxidant activity anti-aging activity |
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