| 新疆阿魏内生真菌链格孢属菌提取物体外抑制肿瘤增殖活性筛选 |
| 投稿时间：2020-11-15 点此下载全文
|
| 引用本文：赵亚琴,徐倞倞,李晓瑾,樊丛照,朱军,王果平.新疆阿魏内生真菌链格孢属菌提取物体外抑制肿瘤增殖活性筛选[J].中国现代中药,2021,23(11):1911-1915 |
| DOI：10.13313/j.issn.1673-4890.20201115002 |
| 摘要点击次数: 460 |
| 全文下载次数: 128 |
| 作者中文名 | 作者英文名 | 单位中文名 | 单位英文名 | E-Mail |
| 赵亚琴 |
ZHAO Ya-qin |
新疆维吾尔自治区中药民族药研究所，新疆 乌鲁木齐 830002
国家中医药管理局 新疆中药民族药资源重点研究室，新疆 乌鲁木齐 830002 |
Xinjiang Institute of Chinese Materia Medica and Ethnical Materia, Urumqi 830002, China
Xinjiang Key Laboratory of Chinese Materia Medica and Ethnic Materia Medica, National Administration of Traditional Chinese Medicine, Urumqi 830002, China |
|
| 徐倞倞 |
XU Liang-liang |
新疆农业大学，新疆 乌鲁木齐 830052 |
Xinjiang Agricultural University, Urumqi 830052, China |
|
| 李晓瑾* |
LI Xiao-jin |
新疆维吾尔自治区中药民族药研究所，新疆 乌鲁木齐 830002
国家中医药管理局 新疆中药民族药资源重点研究室，新疆 乌鲁木齐 830002 |
Xinjiang Institute of Chinese Materia Medica and Ethnical Materia, Urumqi 830002, China
Xinjiang Key Laboratory of Chinese Materia Medica and Ethnic Materia Medica, National Administration of Traditional Chinese Medicine, Urumqi 830002, China |
|
| 樊丛照 |
FAN Cong-zhao |
新疆维吾尔自治区中药民族药研究所，新疆 乌鲁木齐 830002
国家中医药管理局 新疆中药民族药资源重点研究室，新疆 乌鲁木齐 830002 |
Xinjiang Institute of Chinese Materia Medica and Ethnical Materia, Urumqi 830002, China
Xinjiang Key Laboratory of Chinese Materia Medica and Ethnic Materia Medica, National Administration of Traditional Chinese Medicine, Urumqi 830002, China |
|
| 朱军 |
ZHU Jun |
新疆维吾尔自治区中药民族药研究所，新疆 乌鲁木齐 830002
国家中医药管理局 新疆中药民族药资源重点研究室，新疆 乌鲁木齐 830002 |
Xinjiang Institute of Chinese Materia Medica and Ethnical Materia, Urumqi 830002, China
Xinjiang Key Laboratory of Chinese Materia Medica and Ethnic Materia Medica, National Administration of Traditional Chinese Medicine, Urumqi 830002, China |
|
| 王果平 |
WANG Guo-ping |
新疆维吾尔自治区中药民族药研究所，新疆 乌鲁木齐 830002
国家中医药管理局 新疆中药民族药资源重点研究室，新疆 乌鲁木齐 830002 |
Xinjiang Institute of Chinese Materia Medica and Ethnical Materia, Urumqi 830002, China
Xinjiang Key Laboratory of Chinese Materia Medica and Ethnic Materia Medica, National Administration of Traditional Chinese Medicine, Urumqi 830002, China |
|
|
| 基金项目:新疆维吾尔自治区自然科学基金项目（2017D01B48） |
|
| 中文摘要:目的 评价新疆阿魏内生真菌提取物体外抗肿瘤细胞增殖活性，并筛选具有抗肿瘤细胞增殖活性的菌株，为进一步研究其抗肿瘤作用提供依据。方法 采用四甲基偶氮唑盐（MTT）法测定117株新疆阿魏内生真菌菌液提取物和菌丝体提取物对人宫颈癌HeLa细胞和胃癌AGS细胞增殖的影响。结果 有43株链格孢属菌株的49份提取物在质量浓度为100 μg·mL–1时，对肿瘤细胞抑制率达50%以上；其中15份对HeLa细胞、AGS细胞的增殖均具有显著抑制作用，且1938/TL168菌株提取物对HeLa细胞增殖的半数抑制浓度（IC50）低至（25.95±3.39）μg·mL–1。结论 阿魏内生真菌提取物可有效抑制肿瘤细胞增殖，具备抗肿瘤药物研发潜质。 |
| 中文关键词:新疆阿魏 内生真菌 提取物 肿瘤细胞 增殖 体外筛选 |
| |
| Screening of Alternaria Endophytic Fungi from Ferula sinkiangensis Against Tumor Proliferation In vitro |
|
|
| Abstract:Objective This study aims to evaluate the anti-tumor activity of the extracts of endophytic fungi from Ferula sinkiangensis K.M. Shen, and screen the anti-tumor strains, which is expected to lay a theoretical basis for further research on the anti-tumor activity of the fungi.Methods The methyl thiazolyl tetrazolium (MTT) assay was employed to examine the influence of the extracts of the culture and mycelia of 117 endophytic fungal strains from F. sinkiangensis on the proliferation of cervix cancer HeLa cells and gastric cancer AGS cells.Results The inhibition rate of 49 extracts from 43 Alternaria strains on tumor cells reached over 50% at the concentration of 100 μg·mL–1, and 15 of the extracts showed significant suppression on the proliferation of both HeLa cells and AGS cells. Meanwhile, the half-maximal inhibitory concentration (IC50) of 1938/TL168 extract on HeLa cells was only (25.95±3.39) μg·mL–1.Conclusion The extract of F. sinkiangensis endophytic fungi can effectively inhibit the proliferation of tumor cells, which can be used for the development of anti-tumor drugs. |
| keywords:Ferula sinkiangensis K.M.Shen endophytic fungi extract tumor cell proliferation screening in vitro |
|
查看全文
查看/发表评论 下载PDF阅读器 |
|
|
|
