| 金铁锁种子质量检验方法与分级标准 |
| 投稿时间：2020-09-08 点此下载全文
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| 引用本文：熊永兴,曾宇君,苏钛,李云,周国华,杨成金.金铁锁种子质量检验方法与分级标准[J].中国现代中药,2021,23(11):1942-1948 |
| DOI：10.13313/j.issn.1673-4890.20200908004 |
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| 作者中文名 | 作者英文名 | 单位中文名 | 单位英文名 | E-Mail |
| 熊永兴* |
XIONG Yong-xing |
云南省药物研究所/云南省中药和民族药新药创制企业重点实验室，云南 昆明 650111
云南白药集团中药资源有限公司，云南 昆明 650111 |
Yunnan Institute of Materia Medica/Yunnan Province Company Key Laboratory for Traditional Chinese Medicine and Ethnic Drug of New Drug Creation, Kunming 650111, China
Yunnan Baiyao Group Chinese Medicinal Resources Co., Ltd., Kunming 650111, China |
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| 曾宇君 |
ZENG Yu-jun |
云南省药物研究所/云南省中药和民族药新药创制企业重点实验室，云南 昆明 650111 |
Yunnan Institute of Materia Medica/Yunnan Province Company Key Laboratory for Traditional Chinese Medicine and Ethnic Drug of New Drug Creation, Kunming 650111, China |
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| 苏钛 |
SU Tai |
云南省药物研究所/云南省中药和民族药新药创制企业重点实验室，云南 昆明 650111 |
Yunnan Institute of Materia Medica/Yunnan Province Company Key Laboratory for Traditional Chinese Medicine and Ethnic Drug of New Drug Creation, Kunming 650111, China |
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| 李云 |
LI Yun |
云南白药集团中药资源有限公司，云南 昆明 650111 |
Yunnan Baiyao Group Chinese Medicinal Resources Co., Ltd., Kunming 650111, China |
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| 周国华 |
ZHOU Guo-hua |
云南白药集团丽江药业有限公司，云南 丽江 674100 |
Yunnan Baiyao Group Pharmaceutical Co., Ltd., Lijiang 674100, China |
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| 杨成金 |
YANG Cheng-jin |
云南白药集团中药资源有限公司，云南 昆明 650111 |
Yunnan Baiyao Group Chinese Medicinal Resources Co., Ltd., Kunming 650111, China |
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| 基金项目:云南省应用基础研究计划项目（201501YH00081） |
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| 中文摘要:目的 制定金铁锁种子质量检验方法，建立金铁锁种子分级标准。方法 参照《农作物种子检验规程》（GB/T 3543.3—1995），建立金铁锁种子真实性、净度、千粒质量、含水量、生活力、发芽率检验方法，通过K-均值聚类分析36个不同产地金铁锁种子，制定质量分级标准。结果 金铁锁种子净度分析样品应不少于10 g，送检样品应不少于100 g；种子真实性鉴定应采用外观形态观察法及外观形态测量法；种子质量测定应采用千粒法；种子含水量测定采用（130±2）℃恒温烘干4 h；生活力测定采用2，3，5-三苯基氯化四氮唑（TTC）染色法，最佳条件为30 ℃下0.01% TTC染色6 h；发芽率测定应采用滤纸发芽床，经2.5% NaClO浸泡10 min后，于20 ℃恒温光照培养，发芽计数时间为4~16 d。不同来源合格金铁锁种子可分为3个等级，以发芽率和生活力为质量分级标准的主要指标、千粒质量和净度为参考指标。结论 本研究制定的金铁锁种子质量分级标准和等级评定方法科学、可行，可为金铁锁种子质量评价提供参考。 |
| 中文关键词:金铁锁种子 质量检验 分级标准 |
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| Quality Evaluation and Grading Standard of Psammosilene tunicoides Seeds |
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| Abstract:Objective To determine the quality test methods and establish the grading standard for Psammosilene tunicoides seeds.Methods The test methods for sampling amount, authenticity, purity, seed weight, moisture content, viability, and germination rate of P. tunicoides seeds were established according to the Rules for Agricultural Seed Testing (GB/T 3543.3—1995). The quality of seeds from 36 producing areas was determined for the formulation of quality grading standard by K-means clustering.Results At least 100 g seeds should be sampled for testing and no less than 10 g for purity analysis. Seed weight should be measured based on the 1 000-seed weight. For the moisture content, seeds should be dried at (130 ± 2) ℃ for 4 h. The viability should be measured by 0.01% 2,3,5-triphenyltetrazolium chloride (TTC) staining (30 ℃, 6 h). In terms of the germination rate, seeds should be first soaked in 2.5% NaClO for 10 min and then cultured on filter paper (20 ℃, in light, 4-16 days). Seeds from 36 producing areas were classified into three grades with the main indexes of germination rate and viability and reference indexes of 1000-seed weight and purity.Conclusion The seed quality grading standard and quality testing methods were scientific and viable, which can serve as a reference for quality evaluation of P. tunicoides seeds. |
| keywords:Psammosilene tunicoides seeds quality test grading standard |
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