| Abstract:Objective To establish a high performance liquid chromatography (HPLC) method for the simultaneous determination of morroniside, loganin, paeoniflorin, paeonol, chlorogenic acid, galuteolin, 3,5-O-dicaffeoylquinic acid in Qiju Dihuang Pills.Methods HPLC conditions are as below: Inertsil ODS-3 C18 column (250 mm×4.6 mm, 5 μm), gradient elution with acetonitrile-methanol-0.2% glacial acetic acid at the flow rate of 0.9 mL·min–1, column temperature of 30 ℃, injection volume of 10 μL, detection wavelengths of 240 nm (morroniside, loganin, paeoniflorin), 274 nm (paeonol), and 348 nm (chlorogenic acid, galuteolin, 3, 5-O-dicaffeoylquinic acid).Results Morroniside, loganin, paeoniflorin, paeonol, chlorogenic acid, galuteolin, and 3,5-O-dicaffeoylquinic acid showed good linear relationship within the concentration ranges of 4.83-482.66 (r=1.000 0), 5.02-501.95 (r=1.000 0), 4.89-489.33 (r=1.000 0), 10.38-207.63 (r=1.000 0), 1.26-125.83 (r=0.999 6), 0.63-62.98 (r=0.999 7), and 2.34-233.99 μg·mL-1 (r=0.999 8), respectively. The average recoveries were 99.73%, 101.00%, 98.67%, 99.19%, 102.56%, 101.66%, and 100.98%, with the RSDs of 1.29%, 0.51%, 1.21%, 1.01%, 0.97%, 1.07%, and 0.47%, respectively.Conclusion The method is simple, effective, and accurate, which can be used for quality control of Qiju Dihuang Pills. |
