| 基于UPLC-Q-TOF-MS/MS的皖贝母生物碱类化学成分分析 |
| 投稿时间：2021-09-02 点此下载全文
|
| 引用本文：李莉娅,蒲婧哲,张亚中,杨青山,程杰,胡冲.基于UPLC-Q-TOF-MS/MS的皖贝母生物碱类化学成分分析[J].中国现代中药,2022,24(5):792-798 |
| DOI：10.13313/j.issn.1673-4890.20210902005 |
| 摘要点击次数: 211 |
| 全文下载次数: 42 |
| 作者中文名 | 作者英文名 | 单位中文名 | 单位英文名 | E-Mail |
| 李莉娅 |
LI Li-ya |
安徽中医药大学 药学院，安徽 合肥 230012 |
School of Pharmacy, Anhui University of Chinese Medicine, Hefei 230012, China |
|
| 蒲婧哲 |
PU Jing-zhe |
安徽省食品药品检验研究院，安徽 合肥 230000 |
Anhui Institute for Food and Drug Control, Hefei 230000, China |
|
| 张亚中* |
ZHANG Ya-zhong |
安徽中医药大学 药学院，安徽 合肥 230012
安徽省食品药品检验研究院，安徽 合肥 230000 |
School of Pharmacy, Anhui University of Chinese Medicine, Hefei 230012, China
Anhui Institute for Food and Drug Control, Hefei 230000, China |
|
| 杨青山 |
YANG Qing-shan |
安徽中医药大学 药学院，安徽 合肥 230012
安徽省中医药科学院 中药资源保护与开发研究所，安徽 合肥 230012
安徽道地中药材品质提升协同创新中心，安徽 合肥 230012 |
School of Pharmacy, Anhui University of Chinese Medicine, Hefei 230012, China
Institute of Conservation and Development of Traditional Chinese Medicine Resources, Anhui Academy of Chinese Medicine, Hefei 230012, China
Synergetic Innovation Center of Anhui Authentic Chinese Medicine Quality Improvement, Hefei 230012, China |
|
| 程杰 |
CHENG Jie |
安徽省食品药品检验研究院，安徽 合肥 230000 |
Anhui Institute for Food and Drug Control, Hefei 230000, China |
|
| 胡冲 |
HU Chong |
安徽省食品药品检验研究院，安徽 合肥 230000 |
Anhui Institute for Food and Drug Control, Hefei 230000, China |
|
|
| 基金项目:国家重点研发计划项目（2019YFC1711500） |
|
| 中文摘要:目的 采用超高效液相色谱串联四级杆飞行时间质谱法（UPLC-Q-TOF-MS/MS）对皖贝母醇提取物的生物碱类化学成分进行分析鉴定。方法 采用CORTECS C18色谱柱（150 mm×4.6 mm，2.7 μm），流动相为乙腈-0.1%甲酸和10 mmol·L–1甲酸铵水溶液，梯度洗脱，流速为0.40 mL·min–1，进样体积为2 μL。质谱使用电喷雾离子源（ESI），正离子模式扫描采集数据。结果 通过二级高分辨质谱分析结合对照品数据及相关文献，共鉴定出皖贝母醇提取物中14个化合物的结构，包括生物碱类成分12个、非生物碱类2个，其中8个成分为首次从皖贝母中鉴定出来。结论 采用UPLC-Q-TOF-MS/MS建立了快速分析皖贝母中生物碱类化学成分的方法，该法快速、准确，为皖贝母生物碱化学成分鉴定提供了新的策略，并为皖贝母质量控制和物质基础研究提供了参考。 |
| 中文关键词:皖贝母 超高效液相色谱串联四级杆飞行时间质谱法 生物碱 |
| |
| Analysis of Alkaloids from Bulbs of Fritillaria anhuiensis Based on UPLC-Q-TOF-MS/MS |
|
|
| Abstract:Objective To analyze and identify the alkaloid components from the alcoholic extract of bulbs of Fritillaria anhuiensis by ultra performance liquid chromatography tandem quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS/MS).Methods The chromatographic separation was conducted on a CORTECS C18 column (150 mm×4.6 mm, 2.7 μm) with gradient elution of acetonitrile-0.1% formic acid and 10 mmol·L–1 ammonium formate solution as mobile phases at a flow rate of 0.40 mL·min–1 and an injection volume of 2 μL. The mass spectrometry was performed using an electrospray ionization (ESI) source with positive ion mode scan for data acquisition.Results By combining the secondary high-resolution mass spectrometry with the control data and related literature, we identified the structures of 14 compounds, including 12 alkaloids and 2 non-alkaloids, among which 8 components were identified for the first time from bulbs of F. anhuiensis.Conclusion The method is rapid and accurate, which provides a new strategy for the identification of alkaloid components from bulbs of F. anhuiensis, and provides technical support for their further quality control and material basis research. |
| keywords:bulbs of Fritillaria anhuiensis UPLC-Q-TOF-MS/MS alkaloids |
|
查看全文
查看/发表评论 下载PDF阅读器 |
|
|
|
