| 五味子药材提取工艺优化及8个木脂素类成分含量测定 |
| 投稿时间：2021-09-01 点此下载全文
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| 引用本文：朱琳,侯丽华,刘亮亮,陈素娥.五味子药材提取工艺优化及8个木脂素类成分含量测定[J].中国现代中药,2022,24(5):861-867 |
| DOI：10.13313/j.issn.1673-4890.20210901003 |
| 摘要点击次数: 236 |
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| 基金项目:山西省重点研发计划项目（201903D321213） |
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| 中文摘要:目的 优化木脂素类成分的提取方法，并建立高效液相色谱法（HPLC）测定五味子药材中8个木脂素类成分（五味子醇甲、五味子醇乙、当归酰戈米辛H、五味子酯甲、五味子酯乙、五味子甲素、五味子乙素和五味子丙素）的含量测定方法。方法 采用Box-Behnken响应面法，以超声时间、甲醇体积分数、料液比为考察因素，以8个木脂素类成分总含量为考察指标来优化提取工艺，并验证此工艺。采用Agilent ZB-Extend C18色谱柱（250 mm×4.6 mm，5 μm），流动相为0.5%磷酸水溶液-乙腈，梯度洗脱，流速为1 mL·min–1，检测波长为220 nm，柱温为35 ℃。结果 五味子药材中木脂素类成分的最优提取工艺为超声时间30 min，甲醇体积分数为100%，料液比1∶50；不同产地五味子的8个木脂素类成分含量有差异，RSD为8.44%~20.02%。结论 Box-Behnken响应面优化五味子中木脂素的提取工艺科学稳定、准确可靠，建立测定8个木脂素类成分含量的方法专属性强、重复性好、简便准确，可为五味子药材的质量评价及工业化提取生产提供参考。 |
| 中文关键词:五味子 木脂素 含量测定 Box-Behnken响应面法 提取工艺 |
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| Optimization of Extraction Process and Determination of Eight Effective Components in Schisandrae Chinensis Fructus |
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| Abstract:Objective Optimize the extraction process and then to establish a high performance liquid chromatography (HPLC) method for determining the content of eight lignans (schisandrol A, schisandrol B, gomisin H, schisantherin A, schisantherin B, schisandrin A, schisandrin B, and schisandrin C).Methods Box-Behnken design was used to optimize the extraction process. To be specific, the optimal sonication time, methanol concentration, and material-to-liquid ratio in the extraction process were investigated with the content of the eight components as evaluation index. The optimal process was then validated. HPLC conditions are as follows: Agilent ZB-Extend C18 column (250 mm×4.6 mm, 5 μm), column temperature of 35 ℃, mobile phase of 0.5% phosphoric acid solution-acetonitrile for gradient elution at the flow rate of 1 mL·min–1, detection wavelength of 220 nm.Results The optimal extraction process is as below: sonication for 30 min, 100% methanol as solvent, and material-to-liquid ratio of 1∶50. The content of the 8 lignans in Schisandrae Chinensis Fructus of different producing areas was different, with the RSD of 8.44%-20.02%.Conclusion The optimized extraction process is scientific, stable, accurate and reliable. The established method for determining the content of 8 components is highly specific, reproducible, simple and accurate. The result of this study can serve as a reference for quality evaluation and industrial extraction of Schisandrae Chinensis Fructus. |
| keywords:Schisandrae Chinensis Fructus lignans content determination Box-Behnken response surface methodology extraction process |
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