总评归一法结合响应面法优化补肾强筋丸提取工艺
投稿时间:2021-07-23     点此下载全文
引用本文:崔旭辉,尚佳,李喜香,陈国华,周婷婷,刘永昌.总评归一法结合响应面法优化补肾强筋丸提取工艺[J].中国现代中药,2022,24(5):868-875
DOI:10.13313/j.issn.1673-4890.20210723004
摘要点击次数: 177
全文下载次数: 25
                 
作者中文名作者英文名单位中文名单位英文名E-Mail
崔旭辉 CUI Xu-hui 甘肃中医药大学 药学院,甘肃 兰州 730000 College of Pharmacy, Gansu University of Chinese Medicine, Lanzhou 730000, China  
尚佳 SHANG Jia 甘肃省中医院 药学部,甘肃 兰州 730050 Department of Pharmacy, Gansu Provincial Hospital of Traditional Chinese Medicine, Lanzhou 730050, China  
李喜香* LI Xi-xiang 甘肃省中医院 药学部,甘肃 兰州 730050 Department of Pharmacy, Gansu Provincial Hospital of Traditional Chinese Medicine, Lanzhou 730050, China  
陈国华 CHEN Guo-hua 甘肃省疾病预防控制中心,甘肃 兰州 730000 Gansu Provincial Center for Disease Control and Prevention, Lanzhou 730000, China  
周婷婷 ZHOU Ting-ting 甘肃中医药大学 药学院,甘肃 兰州 730000 College of Pharmacy, Gansu University of Chinese Medicine, Lanzhou 730000, China  
刘永昌 LIU Yong-chang 甘肃中医药大学 药学院,甘肃 兰州 730000 College of Pharmacy, Gansu University of Chinese Medicine, Lanzhou 730000, China  
基金项目:甘肃省中医药科研课题(GZKP-2020-27)
中文摘要:目的 优化补肾强筋丸的提取工艺。方法 采用高效液相色谱法,以地黄苷D、马钱苷、莫诺苷、阿魏酸含量和浸膏得率的总评归一值(OD值)为评价指标,以浸泡时间、提取时间、溶剂量为影响因素,应用星点设计-效应面法选取最佳提取工艺条件。结果 最佳提取工艺为12倍水提取2次,每次浸泡时间为60 min,提取时间为120 min,OD值为0.723 3。验证实验结果显示,实测值与预测值偏差为3.0%。结论 该方法稳定可行,可用于提取补肾强筋丸,为临床应用和新药的研发提供参考。
中文关键词:补肾强筋丸  提取工艺  多指标  高效液相色谱法  归一化法
 
Extraction Process Optimization for Preparation of Bushen Qiangjin Pills by Overall Desirability and Response Surface Methodology
Abstract:Objective To optimize the extraction process of Chinese medicinal materials for the preparation of Bushen Qiangjin pills.Methods High performance liquid chromatography was employed to determine the content of active components. The optimal extraction conditions were determined based on the overall desirability (OD) of rehmannioside D, loganin, morroniside, ferulic acid, and extract yield. The optimum soaking time, extraction time, and solvent volume were determined via central composite design-response surface methodology.Results The optimum extraction conditions: 12 volumes of water, extraction for twice, soaking time of 60 min, and extraction time of 120 min, under which the OD was 0.723 3. The results of validation test showed that the deviation between the predicted value and the measured value was 3.0%.Conclusion The optimized extraction conditions are stable and feasible and can be used for the preparation of Bushen Qiangjin pills, which provide reference for clinical application as well as the research and development of new drugs.
keywords:Bushen Qiangjin pills  extraction process  multiple indicators  HPLC  overall desirability
查看全文   查看/发表评论  下载PDF阅读器