| 《中华人民共和国药典》2020年版收载何首乌和制何首乌质量标准【鉴别】项的商榷 |
| 投稿时间：2022-01-25 点此下载全文
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| 引用本文：王雪婷,杨建波,程显隆,汪祺,高慧宇,宋云飞,王莹,魏锋,马双成.《中华人民共和国药典》2020年版收载何首乌和制何首乌质量标准【鉴别】项的商榷[J].中国现代中药,2022,24(10):1877-1885 |
| DOI：10.13313/j.issn.1673-4890.20220125003 |
| 摘要点击次数: 396 |
| 全文下载次数: 79 |
| 作者中文名 | 作者英文名 | 单位中文名 | 单位英文名 | E-Mail |
| 王雪婷 |
WANG Xue-ting |
中国食品药品检定研究院，北京 100050 |
National Institutes for Food and Drug Control, Beijing 100050, China |
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| 杨建波 |
YANG Jian-bo |
中国食品药品检定研究院，北京 100050 |
National Institutes for Food and Drug Control, Beijing 100050, China |
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| 程显隆 |
CHENG Xian-long |
中国食品药品检定研究院，北京 100050 |
National Institutes for Food and Drug Control, Beijing 100050, China |
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| 汪祺 |
WANG Qi |
中国食品药品检定研究院，北京 100050 |
National Institutes for Food and Drug Control, Beijing 100050, China |
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| 高慧宇 |
GAO Hui-yu |
中国食品药品检定研究院，北京 100050 |
National Institutes for Food and Drug Control, Beijing 100050, China |
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| 宋云飞 |
SONG Yun-fei |
中国食品药品检定研究院，北京 100050 |
National Institutes for Food and Drug Control, Beijing 100050, China |
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| 王莹 |
WANG Ying |
中国食品药品检定研究院，北京 100050 |
National Institutes for Food and Drug Control, Beijing 100050, China |
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| 魏锋* |
WEI Feng |
中国食品药品检定研究院，北京 100050 |
National Institutes for Food and Drug Control, Beijing 100050, China |
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| 马双成 |
MA Shuang-cheng |
中国食品药品检定研究院，北京 100050 |
National Institutes for Food and Drug Control, Beijing 100050, China |
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| 基金项目:国家重点研发计划项目（2019YFC1711500）；国家自然科学基金项目（81973476） |
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| 中文摘要:目的 讨论完善《中华人民共和国药典》（以下简称《中国药典》）2020年版何首乌及制何首乌的薄层色谱鉴别项。方法 考察超声及加热回流2种不同的样品制备方式，以及用二氯甲烷-甲醇-甲酸、乙酸乙酯-石油醚-甲酸展开系统代替原标准中三氯甲烷-甲醇展开系统的可行性，同时比较了不同显色条件。结果 2种制备方式制得的样品在相同的展开条件下展开结果并没有明显差异，超声提取操作更加简便；以二氯甲烷-甲醇-甲酸（5∶1∶0.3）和石油醚-乙酸乙酯-甲酸（5.0∶1.0∶0.3）代替三氯甲烷-甲醇展开系统后，喷10%硫酸乙醇显色剂并在紫外灯365 nm下检视，薄层色谱板上斑点明显增多且分离效果好，较《中国药典》2020年版何首乌和制何首乌薄层色谱鉴别条件更理想。结论 改进后的方法毒性低、简便易行、可行性良好，为进一步完善《中国药典》2020年版何首乌和制何首乌质量标准提供参考。 |
| 中文关键词:《中国药典》 何首乌 制何首乌 提取方式 薄层色谱 |
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| Discussion on Identification of Polygoni Multiflori Radix and Polygoni Multiflori Radix Praeparata in Chinese Pharmacopoeia (2020 edition) |
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| Abstract:Objective To discuss and improve the identification of Polygoni Multiflori Radix and Polygoni Multiflori Radix Praeparata by thin layer chromatography (TLC) in Chinese Pharmacopoeia (2020 edition).Methods For sample extraction, ultrasonic extraction and heating reflux were compared. In addition, whether it was feasible to replace the trichloromethane-methanol solvent system in the standard with dichloromethane-methanol-formic acid system and ethyl acetate-petroleum ether-formic acid system was investigated. Besides, the condition of color reaction was also studied.Results Under the same unfolding conditions, there is no significant difference in the unfolding results of the samples prepared by the two preparation methods, and the ultrasonic extraction operation is more convenient. When dichloromethane-methanol-formic acid (5:1:0.3) system and petroleum ether-ethyl acetate-formic acid (5:1:0.3) system were used instead of the dichloromethane-methanol system in Chinese Pharmacopoeia (2020 edition), the spots on TLC plate increased obviously with better separation effect after being sprayed with chromogenic agent.Conclusion The improved method features low toxicity and ease of implementation, which is feasible. The findings in this study can serve as a reference for the further improving the standard of Polygoni Multiflori Radix and Polygoni Multiflori Radix Praeparata in Chinese Pharmacopoeia (2020 edition). |
| keywords:Chinese Pharmacopoeia Polygoni Multiflori Radix Polygoni Multiflori Radix Praeparata extraction method thin layer chromatography (TLC) |
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