羟丙基甲基纤维素对盐酸小檗碱肠道微环境调控效应的影响
投稿时间:2022-03-07     点此下载全文
引用本文:吴晨阳,黎迎,邹佩志,郑婷婷,张运,董政起.羟丙基甲基纤维素对盐酸小檗碱肠道微环境调控效应的影响[J].中国现代中药,2022,24(10):1893-1901
DOI:10.13313/j.issn.1673-4890.20220307001
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作者中文名作者英文名单位中文名单位英文名E-Mail
吴晨阳 WU Chen-yang 中国医学科学院 北京协和医学院 药用植物研究所,北京 100193 Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, China  
黎迎 LI Ying 中国医学科学院 北京协和医学院 药用植物研究所,北京 100193 Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, China  
邹佩志 ZOU Pei-zhi 中国医学科学院 北京协和医学院 药用植物研究所,北京 100193 Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, China  
郑婷婷 ZHENG Ting-ting 中国医学科学院 北京协和医学院 药用植物研究所,北京 100193 Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, China  
张运 ZHANG Yun 中国医学科学院 北京协和医学院 药用植物研究所,北京 100193 Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, China  
董政起* DONG Zheng-qi 中国医学科学院 北京协和医学院 药用植物研究所,北京 100193 Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, China  
基金项目:北京协和医学院“中央高校基本科研业务费”项目(3332021051);中国医学科学院医学与健康科技创新工程项目(2021-I2M-1-031,2021-I2M-1-022)
中文摘要:目的 探讨羟丙基甲基纤维素(HPMC)与盐酸小檗碱(BBR)合用时是否会对其肠道微环境调控效应产生影响。方法 选用C57BL/6雄性小鼠,分为对照组、HPMC(400 mg·kg–1)组、BBR(150 mg·kg–1)组、HPMC与BBR物理混合(H+B,400 mg·kg–1+150 mg·kg–1)组,进行连续3周灌胃干预。取小鼠远端结肠,苏木精-伊红(HE)染色法检测结肠组织病理学形态;采用16S rRNA基因测序探究小鼠肠道菌群结构变化。结果 与对照组比较,BBR组小鼠肠道菌群Alpha多样性显著降低,Beta多样性结果表明菌群组成显著改变,变形菌门(Proteobacteria)、疣微菌门(Verrucomicrobia)及艾克曼菌属(Akkermansia)相对丰度显著提高。而HPMC与BBR物理混合给药后能够进一步促进BBR引起的小鼠肠道菌群Alpha多样性降低趋势,增强其抑菌效果;在Beta多样性方面,H+B组与BBR组的肠道菌群组成产生差异,同时显著增强了BBR富集变形菌门、疣微菌门和艾克曼菌属的作用。结论 HPMC与BBR物理混合后可正向促进BBR的菌群调节作用,推测原因为其高黏性能够增强小檗碱在肠道上的黏附,从而使小檗碱与肠道菌群充分接触。
中文关键词:羟丙基甲基纤维素  盐酸小檗碱  肠道菌群  肠道微环境
 
Effect of Hydroxypropyl Methylcellulose as a Pharmaceutical Excipient on Berberine Hydrochloride Regulating Intestinal Microenvironment
Abstract:Objective To explore the effect of hydroxypropyl methylcellulose (HPMC) on berberine hydrochloride (BBR) regulating intestinal microenvironment.Methods C57BL/6 male mice were divided into the control group, HPMC group (400 mg·kg–1), BBR group (150 mg·kg–1), and HPMC+BBR (400 mg·kg–1+150 mg·kg–1). All groups received a three-week continuous drug intervention (i.g). Then hematoxylin and eosin (HE) staining was used to analyze the histopathological change in the mouse distal colon and 16S rRNA gene sequencing was performed to explore the structural changes in mouse intestinal flora.Results Compared with the conditions in the control group, the alpha diversity of the intestinal flora in the BBR group decreased significantly, and the beta diversity analysis showed a remarkable change in the composition of the microflora, with the relative abundance of Proteobacteria, Verrucomicrobia and Akkermansia significantly improved. Furthermore, the physical mixture of HPMC and BBR further promoted the trend of decreasing alpha diversity of intestinal flora induced by BBR and enhanced its antibacterial effect. Difference was observed between the HPMC+BBR group and BBR group in the microbial composition. Moreover, the role of BBR in enriching Proteobacteria, Verrucomicrobia and Akkermansia was further strengthened.Conclusion The function of BBR regulating gut microbiota would be positively enhanced by physically mixing with HPMC. It was inferred the mucoadhesion of BBR on the intestine was increased, and thus it was in full contact with the intestinal flora.
keywords:hydroxypropyl methylcellulose  berberine hydrochloride  gut microbiota  intestinal microenvironment
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