草甘膦对党参生长 根腐病及根际土壤微生物的影响
投稿时间:2021-06-04     点此下载全文
引用本文:杨姗姗,仲彩萍,王仪,刘紫祺,邵慧慧,孙新荣,高微微.草甘膦对党参生长 根腐病及根际土壤微生物的影响[J].中国现代中药,2022,24(10):1932-1938
DOI:10.13313/j.issn.1673-4890.20210604005
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作者中文名作者英文名单位中文名单位英文名E-Mail
杨姗姗 YANG Shan-shan 中国医学科学院 北京协和医学院 药用植物研究所,北京 100193 Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, China  
仲彩萍 ZHONG Cai-ping 甘肃省定西市渭源县农业技术推广中心,甘肃 定西 748200 Weiyuan County Agricultural Technology Promotion Center, Dingxi 748200, China  
王仪 WANG Yi 中国医学科学院 北京协和医学院 药用植物研究所,北京 100193
中草药物质基础与资源利用教育部重点实验室,北京 100193
Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, China
Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education, Beijing 100193, China
 
刘紫祺 LIU Zi-qi 中国医学科学院 北京协和医学院 药用植物研究所,北京 100193
中草药物质基础与资源利用教育部重点实验室,北京 100193
Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, China
Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education, Beijing 100193, China
 
邵慧慧 SHAO Hui-hui 中国医学科学院 北京协和医学院 药用植物研究所,北京 100193
中草药物质基础与资源利用教育部重点实验室,北京 100193
Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, China
Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education, Beijing 100193, China
 
孙新荣 SUN Xin-rong 甘肃省定西市渭源县农业技术推广中心,甘肃 定西 748200 Weiyuan County Agricultural Technology Promotion Center, Dingxi 748200, China  
高微微* GAO Wei-wei 中国医学科学院 北京协和医学院 药用植物研究所,北京 100193
中草药物质基础与资源利用教育部重点实验室,北京 100193
Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, China
Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education, Beijing 100193, China
 
基金项目:甘肃省科技计划项目(21CX6NJ206);中国医学科学院医学与健康科技创新工程项目(2021-I2M-1-031)
中文摘要:目的 探究除草剂草甘膦对党参生长、根腐病发生和药用成分的影响及可能的土壤微生态机制。方法 调查同一地块中草甘膦处理组和对照组党参的存苗数、鲜质量和发病情况,采用高效液相色谱法(HPLC)测定党参炔苷含量、分光光度法测定多糖含量,采用扩增子技术测定根际土壤微生物群落变化。结果 施用草甘膦能够显著降低党参的存苗数(P<0.05),对党参地上部生长有一定抑制作用;根腐病发病率及病情指数高于对照组,分别增加了34.51%和34.81%(P<0.05);党参炔苷和多糖的含量降低。另外,草甘膦处理对党参根际土壤微生物的多样性及真菌群落结构影响较小,但细菌群落中伯克霍尔德氏菌目(Burkholderiales)和鞘脂单胞菌目(Sphingomonadales)显著增加(P<0.05)。结论 施用草甘膦对党参生长有一定抑制作用,导致根腐病加重,这可能与部分根际细菌的相对丰度变化有关。
中文关键词:党参  草甘膦  生物量  根腐病  根际微生物
 
Effects of Glyphosate on Growth, Root Rot and Rhizosphere Soil Microbes of Codonopsis pilosula
Abstract:Objective To explore the effects of herbicide glyphosate on the growth, root rot occurrence, and medicinal ingredients of Codonopsis pilosula and the possible soil micro-ecological mechanism.Methods This study investigated the number of seedlings, fresh quality and disease status of C. pilosula in glyphosate treatment group and control group in the same C. pilosula planting plot. High performance liquid chromatography (HPLC) and spectrophotometry were used to determine the content of lobetyolin and polysaccharides, respectively, and amplicon sequencing was performed to detect the microbial community in rhizosphere soil.Results The application of glyphosate significantly reduced the number of seedlings of C. pilosula (P<0.05), and also had a certain inhibitory effect on the growth of the aerial parts of C. pilosula. The incidence rate and disease index of root rot in glyphosate treatment group were higher than those of the control group, increasing by 34.51% and 34.81%, respectively (P<0.05), and the content of lobetyolin and polysaccharides decreased. In addition, glyphosate had no remarkable effect on the microbial diversity and fungal community structure in the rhizosphere soil of C. pilosula, but Burkholderiales and Sphingomonadales in the bacterial community increased significantly (P<0.05).Conclusion Glyphosate had a certain inhibitory effect on the growth of C. pilosula, aggravating root rot, which might be related to the relative abundance of some rhizosphere bacteria.
keywords:Codonopsis pilosula (Franch.) Nannf.  glyphosate  biomass  root rot  rhizosphere microbes
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