| 基于HPLC-QAMS多指标成分含量测定联合化学计量学的恒古骨伤愈合剂质量控制 |
| 投稿时间：2022-03-24 点此下载全文
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| 引用本文：冯晓川,徐延昭,张静,张蕊.基于HPLC-QAMS多指标成分含量测定联合化学计量学的恒古骨伤愈合剂质量控制[J].中国现代中药,2022,24(10):1995-2003 |
| DOI：10.13313/j.issn.1673-4890.20220324006 |
| 摘要点击次数: 304 |
| 全文下载次数: 50 |
| 作者中文名 | 作者英文名 | 单位中文名 | 单位英文名 | E-Mail |
| 冯晓川 |
FENG Xiao-chuan |
北京积水潭医院 中药房，北京 100035 |
Traditional Chinese Medicine Pharmacy, Beijing Jishuitan Hospital, Beijing 100035, China |
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| 徐延昭 |
XU Yan-zhao |
中国食品药品检定研究院，北京 100050 |
National Institutes for Food and Drug Control, Beijing 100050, China |
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| 张静 |
ZHANG Jing |
北京积水潭医院 中药房，北京 100035 |
Traditional Chinese Medicine Pharmacy, Beijing Jishuitan Hospital, Beijing 100035, China |
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| 张蕊* |
ZHANG Rui |
北京积水潭医院 中药房，北京 100035 |
Traditional Chinese Medicine Pharmacy, Beijing Jishuitan Hospital, Beijing 100035, China |
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| 基金项目:北京市中医管理局第二批中药骨干人才培养项目（京中医科字〔2018〕213号） |
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| 中文摘要:目的 建立高效液相色谱-一测多评法（HPLC-QAMS）多指标成分含量测定联合化学计量学的恒古骨伤愈合剂质量控制研究的方法。方法 采用HPLC-QAMS检测15批恒古骨伤愈合剂中11个成分的含量，并与外标法结果进行对比，采用聚类分析、主成分分析与偏最小二乘法-判别分析对HPLC-QAMS结果进行分析评价。结果 11个成分分别在各自质量浓度范围内线性关系良好（r>0.999 3）；平均加样回收率为96.81%~100.21%（RSD<2.0%）；HPLC-QAMS所测结果与ESM差异无统计学意义；通过化学计量学分析，羟基红花黄色素A［变量重要性投影（VIP）值=1.446］、三七皂苷R1（VIP值=1.351）、橙皮苷（VIP值=1.258）、京尼平苷（VIP值=1.138）、人参皂苷Rg1（VIP值=1.067）可能是影响恒古骨伤愈合剂产品质量的差异性标志物。结论 建立的方法操作便捷、可靠稳定，可为深入研究恒古骨伤愈合剂质量控制提供参考。 |
| 中文关键词:恒古骨伤愈合剂 多指标成分 高效液相色谱-一测多评法 相对校正因子 化学计量学 综合质量评价 |
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| Comprehensive Quality Evaluation of Henggu Gushangyu Mixture Based on HPLC-QAMS and Chemometrics |
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| Abstract:Objective To establish a high-performance liquid chromatography-quantitative analysis of multi-components by single marker (HPLC-QAMS) method, combined with chemometrics, and study the quality control of Henggu Gushangyu Mixture.Methods Eleven components in 15 batches of Henggu Gushangyu Mixture were determined by HPLC-QAMS. The results were compared with those of external standard method (ESM), and were analyzed and evaluated by cluster analysis (CA), principal component analysis (PCA) and partial least squares discriminant analysis (PLS-DA).Results The 11 components had good linear relationship within their respective ranges (r>0.999 3). The average recoveries were 96.81%-100.21% (RSD<2.0%). There was no significant difference between HPLC-QAMS and ESM. Chemometrics revealed hydroxysafflor yellow A [variable importance in projection (VIP)=1.446], notoginsenoside R1 (VIP=1.351), hesperidin (VIP=1.258), geniposide (VIP=1.138) and ginsenoside Rg1 (VIP=1.067) might be the differential markers affecting the quality of Henggu Gushangyu Mixture.Conclusion The established method was convenient, reliable and stable, and provided reference for further study on the quality of Henggu Gushangyu Mixture. |
| keywords:Henggu Gushangyu Mixture multi-index component HPLC-QAMS RCF chemometrics comprehensive quality evaluation |
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