RP-HPLC同时测定野菊花中绿原酸、木犀草苷和蒙花苷的含量
     点此下载全文
引用本文:郭美兰,敬应春,蔡国琴.RP-HPLC同时测定野菊花中绿原酸、木犀草苷和蒙花苷的含量[J].中国现代中药,2012,14(4):10-13
DOI:
摘要点击次数: 3179
全文下载次数: 2269
        
作者中文名作者英文名单位中文名单位英文名E-Mail
郭美兰 GUO Mei-lan 上海市中药研究所,上海 201401 Shanghai Institute of Chinese Materia Medica,Shanghai 201401, China  
敬应春 JING Ying-chun 上海市中药研究所,上海 201402 Shanghai Institute of Chinese Materia Medica,Shanghai 201402, China  
蔡国琴 CAI Guo-qin 上海市中药研究所,上海 201403 Shanghai Institute of Chinese Materia Medica,Shanghai 201403, China caigq19@126.com 
中文摘要:建立同时测定野菊花中绿原酸、木犀草苷和蒙花苷3个主要活性成分含量的方法。方法:采用RP-HPLC法,选用Kromasil-C18(250 mm×4.6 mm,5 μm,)色谱柱;以乙腈-0.05%磷酸溶液为流动相(梯度洗脱);流速为1.0 mL·min-1;检测波长为334 nm;柱温为30℃。结果:绿原酸、木犀苷和蒙花苷分别在40.44~647.04 ng(r=0.999 8)、19.50~311.92 ng(r=0.999 7)和253.62~405 7.82 ng(r=0.999 9)范围内线性关系良好;平均回收率分别为100.96%、99.99%和98.94%,RSD均<2.0%。22批野菊花药材中绿原酸含量为0.108%~0.545%,木犀苷含量为0.029%~0.393%,蒙花苷含量为0.028%~3.518%。结论:本方法简便准确、稳定可靠,可以用于野菊花药材的含量测定。
中文关键词:野菊花  RP-HPLC法  绿原酸  木犀草苷  蒙花苷
 
RP-HPLC Simultaneous Determination of Chlorogenic Acid,luteolin-7-O-β-D-glucoside and Linarin in Chrysanthemi Indici Flos
Abstract:To establish a method for the determination of three active ingredients,chlorogenic acid,luteolin-7-O-β-D-glucoside,and Linarin in Flos Chrysanthemi Indici. Methods: A Kromasil -C18(250 mm×4.6 mm, 5 μm)column was used with the mobile phase (gradient elution) being acetonitrile-0.05% phosphate acid , flow rate being 1.0 mLomin-1,detecting wavelength being 334 nm,the cloum temperature being 30 ℃.Results: The linear response range from 40.44~647.04 ng(r=0.999 8,n=5)of chlorogenic acid, 19.50~311.92 ng(r=0.999 7,n=5)of luteolin-7-O-β-D-glucoside, 253.62~4 057.82 ng (r=0.999 9,n=5)of Linarin. The average recoveries (n=6) chlorogenic acid,luteolin-7-O-β-D-glucoside,and Linarin were 100.96%,99.99%,and 98.94 % respectively. The average recoveries of chlorogenic acid,luteolin-7-O-β-D-glucoside,and Linarin were 100.96%,99.99%,and 98.94 % with RSD less than 2.0%.Conclusion: The method is simple ,accurate, reproducible and stable , and can be used for the quality control of Flos Chrysanthemi Indici.
keywords:Chrysanthemi Indici Flos  RP- HPLC  Chlorogenic acid  Luteolin-7-O-β-D-glucoside  Linarin
查看全文   查看/发表评论  下载PDF阅读器