| 青钱柳等几种别样茶中咖啡因、可可碱和茶碱的检测 |
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| 引用本文：何春年,彭勇,肖伟,肖培根.青钱柳等几种别样茶中咖啡因、可可碱和茶碱的检测[J].中国现代中药,2012,14(4):14-17 |
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| 作者中文名 | 作者英文名 | 单位中文名 | 单位英文名 | E-Mail |
| 何春年 |
HE Chun-nian |
中国医学科学院 北京协和医学院 药用植物研究所，北京 100193 |
Institute of Medicinal Plant Development,Chinese Academy of Medical Science,Peking Union Medical College,Beijing 100193,China |
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| 彭勇 |
PENG Yong |
国家教育部中草药物质基础与资源利用重点实验室，北京 100193 |
Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine Peking Union Medical College,Ministry of Education,Beijing 100193,China |
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| 肖伟 |
XIAO Wei |
中国医学科学院 北京协和医学院 药用植物研究所，北京 100193 |
Institute of Medicinal Plant Development,Chinese Academy of Medical Science,Peking Union Medical College,Beijing 100193,China |
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| 肖培根 |
XIAO Pei-gen |
国家教育部中草药物质基础与资源利用重点实验室，北京 100193 |
Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine Peking Union Medical College,Ministry of Education,Beijing 100193,China |
xiaopg@public.bta.net.cn |
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| 中文摘要:对青钱柳甜茶等几种别样茶中咖啡因、可可碱和茶碱进行检测，为别样茶的合理应用奠定基础。方法：采用超快速液相色谱法(RSLC)，Acclaim PA2色谱柱(150 mm×2.1 mm,2.2 μm)，乙腈-水梯度洗脱；流速：0.5 mL·min-1；检测波长：273 nm，柱温：30 ℃。结果：除加入绿茶的商品中检测到适量的咖啡因外，青钱柳神茶、黄芩茶、多穗柯甜茶、悬钩子属甜茶，以及甜叶菊叶和湖北海棠叶中均未检测出上述3种生物碱。结论：相对于绿茶中含有较高含量的咖啡因、可可碱和茶碱，别样茶中未检出上述3种成分，为别样茶的科学应用提供指导。 |
| 中文关键词:别样茶 青钱柳 咖啡因 可可碱 茶碱 RSLC |
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| Determination of Caffeine,Theobromine,and Theophylline in the Leaves of Cyclocarya paliurus and Several Other Kinds of Tea |
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| Abstract:To determine caffeine,theobromine,and theophylline contents in the leaves of Cyclocarya paliurus and several other kinds of tea by RSLC.Methods:The separation was carried out through a gradient elution using a Dionex Acclaim RSLC PA2(150 mm×2.1 mm,2.2 μm) column and a mobile phase consisting of water and acetonitrile at a flow rate of 0.5 mL·min-1.The detection wavelength was set at 273 nm.The column temperature was 30 ℃.Results:Under the optimized conditions,all the 3 analytes were successfully separated within 10 min,and good linearity(r>0.999 6)was achieved.This method was also validated with respect to precision,specificity,and was successfully applied to simultaneous determination of 3 compounds in the leaves of Cyclocarya paliurus and several other kinds of tea including:Huang-qin tea,Duo-sui-ke tea,Xuan-gou-zi sweet tea,Tian-ye-ju tea,Hubei-hai-tang tea.The results indicate that the 3 compounds were not detected in the six species of plants.Conclusion:It can guide the application of science for other kinds of tea. |
| keywords:Cyclocarya paliurus Caffeine Theobromine Theophylline RSLC |
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