沉香香粉薰香吸入促睡眠作用及机制研究
投稿时间:2022-03-28     点此下载全文
引用本文:吴玉兰,弓宝,刘洋洋,王灿红,魏建和.沉香香粉薰香吸入促睡眠作用及机制研究[J].中国现代中药,2023,25(1):83-89
DOI:10.13313/j.issn.1673-4890.20220328002
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作者中文名作者英文名单位中文名单位英文名E-Mail
吴玉兰 WU Yu-lan 中国医学科学院 北京协和医院 药用植物研究所海南分所,海南省南药资源保护与开发重点实验室/ 国家中医药管理局沉香可持续利用重点研究室,海南 海口 570311 Hainan Provincial Key Laboratory of Resources Conservation and Development of Southern Medicine, Key Laboratory of State Administration of Traditional Chinese Medicine for Agarwood Sustainable Utilization, Hainan Branch Institute of Medicinal Plant, Chinese Academy of Medical Sciences, Peking Union Medical College, Haikou 570311, China  
弓宝 GONG Bao 中国医学科学院 北京协和医院 药用植物研究所海南分所,海南省南药资源保护与开发重点实验室/ 国家中医药管理局沉香可持续利用重点研究室,海南 海口 570311 Hainan Provincial Key Laboratory of Resources Conservation and Development of Southern Medicine, Key Laboratory of State Administration of Traditional Chinese Medicine for Agarwood Sustainable Utilization, Hainan Branch Institute of Medicinal Plant, Chinese Academy of Medical Sciences, Peking Union Medical College, Haikou 570311, China  
刘洋洋 LIU Yang-yang 中国医学科学院 北京协和医院 药用植物研究所海南分所,海南省南药资源保护与开发重点实验室/ 国家中医药管理局沉香可持续利用重点研究室,海南 海口 570311 Hainan Provincial Key Laboratory of Resources Conservation and Development of Southern Medicine, Key Laboratory of State Administration of Traditional Chinese Medicine for Agarwood Sustainable Utilization, Hainan Branch Institute of Medicinal Plant, Chinese Academy of Medical Sciences, Peking Union Medical College, Haikou 570311, China  
王灿红* WANG Can-hong 中国医学科学院 北京协和医院 药用植物研究所海南分所,海南省南药资源保护与开发重点实验室/ 国家中医药管理局沉香可持续利用重点研究室,海南 海口 570311 Hainan Provincial Key Laboratory of Resources Conservation and Development of Southern Medicine, Key Laboratory of State Administration of Traditional Chinese Medicine for Agarwood Sustainable Utilization, Hainan Branch Institute of Medicinal Plant, Chinese Academy of Medical Sciences, Peking Union Medical College, Haikou 570311, China  
魏建和 WEI Jian-he 中国医学科学院 北京协和医院 药用植物研究所 中草药物质基础与资源利用教育部重点实验室/ 濒危药材繁育国家工程实验室,北京 100193 Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, National Engineering Laboratory for Breeding of Endangered Medicinal Materials, Institute of Medicinal Plant Development, Chinese Academy of Medical Science, Peking Union Medical College, Beijing 100193, China  
基金项目:国家自然科学基金青年基金项目(82204657);海南省重点研发计划项目(ZDYF2022SHFZ030);国家重点研发计划项目(2018YFC1706400);海南省高层次人才项目(2019RC345)
中文摘要:目的 探究沉香香粉加热薰香吸入给药促睡眠的作用及可能的作用机制。方法 健康昆明种小鼠分为正常组、模型组、阳性对照组及沉香粉低、中、高剂量组,每组8只。连续2 d灌胃对氯苯丙氨酸(PCPA,300 mg·kg–1),诱导失眠模型。期间同时给药,正常组和模型组给予相应体积的0.9%氯化钠溶液,阳性对照组腹腔注射地西泮(2.5 mg·kg–1)。沉香低、中、高剂量(0.2、0.4、0.8 g)组采用电子薰香炉120 ℃加热香薰吸入给药,5 h/次。通过协同戊巴比妥钠促睡眠试验,考察沉香香粉吸入给药镇静促睡眠的情况,并检测海马组织神经递质水平及其受体表达情况,探究沉香香薰吸入促睡眠的作用机制。结果 相比正常组,模型组小鼠的睡眠潜伏期延长,睡眠时间显著缩短,动物已出现明显失眠症状,表明模型制备成功。相比模型组,沉香香粉吸入给药显著缩短睡眠潜伏期,延长协同戊巴比妥钠(50 mg·kg–1)促睡眠的时间,有较好的镇静促睡眠作用;机制研究结果显示,相比正常组,模型组小鼠脑组织中神经递质、受体及蛋白表达不同程度降低,而沉香香粉吸入给药后可显著升高γ-氨基丁酸A受体(GABAA)、谷氨酸(Glu)、5-羟色胺(5-HT)和腺苷(AD)水平,同时可显著上调Glu受体1和Glu转运蛋白1(VGluT1)的表达水平。结论 沉香香粉吸入有促睡眠作用,其机制与调节多神经途径及神经递质分泌平衡有关。
中文关键词:沉香香粉  香薰  促睡眠  神经递质  谷氨酸-γ-氨基丁酸系统
 
Sleep-promoting Effect and Mechanism of Agarwood Powder Incense Inhalation
Abstract:Objective To explore the sleep-promoting effect and underlying mechanism of agarwood powder incense inhalation.Methods Healthy Kunming mice were randomly divided into a normal group, a model group, a positive group, and low-, medium-, and high-dose agarwood powder groups, with 8 mice in each group. The insomnia model was induced in mice by intragastric administration of chlorophenylalanine (PCPA, 300 mg·kg–1) for 2 consecutive days. The mice in the normal group and the model group were given the same volume of normal saline, and those in the positive group received diazepam (2.5 mg·kg–1) by intraperitoneal injection. The mice in the low-, medium-, and high-dose (0.2, 0.4, and 0.8 g) agarwood powder groups underwent agarwood powder incense inhalation with the electronic incense burner at 120 ℃, 5 h/time. Through the sleep-promoting test with pentobarbital sodium, the effect of inhalation of agarwood powder on sedation and sleep promotion was investigated, and neurotransmitter levels and receptor expression in hippocampal tissues were detected to explore the mechanism of inhalation of agarwood powder in promoting sleep.Results Compared with the normal group, the model group showed prolonged sleep latency, shortened sleep time, and obvious insomnia symptoms in animals, indicating that the model was successfully established. Compared with the conditions in the model group, inhalation of agarwood powder significantly shortened the sleep latency, extended the time of sleep induced by pentobarbital sodium (50 mg·kg–1), and showed a good sedative and sleep-promoting effect. As revealed by the analysis of mechanism, compared with the normal group, the model group showed decreased expression of neurotransmitters, receptors and proteins in the brain tissues to varying degrees, while inhalation of agarwood powder could significantly increase the levels of γ-aminobutyric acid A receptor (GABAA), glutamic acid (Glu), 5-hydroxytryptamine (5-HT), and adenosine (AD), and significantly up-regulate the expression of Glu receptor 1 (GluR1) and vesicular glutamate transporter 1 (VGluT1).Conclusion Agarwood powder inhalation can promote sleep, and its mechanism is related to the regulation of multiple nerve pathways and neurotransmitter secretion balance.
keywords:agarwood powder  incense inhalation  promote sleep  neurotransmitter  Glu-GABA system
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