ICP-MS测定鲜地黄中26种金属元素及不同加工工艺元素差异性比较
投稿时间:2022-08-08     点此下载全文
引用本文:王珊,许海燕,彭修娟,刘峰.ICP-MS测定鲜地黄中26种金属元素及不同加工工艺元素差异性比较[J].中国现代中药,2023,25(1):99-106
DOI:10.13313/j.issn.1673-4890.20220808002
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作者中文名作者英文名单位中文名单位英文名E-Mail
王珊 WANG Shan 陕西国际商贸学院,陕西 咸阳 712046 Shaanxi Institute of International Trade & Commerce, Xianyang 712046, China  
许海燕 XU Hai-yan 陕西国际商贸学院,陕西 咸阳 712046 Shaanxi Institute of International Trade & Commerce, Xianyang 712046, China  
彭修娟 PENG Xiu-juan 陕西国际商贸学院,陕西 咸阳 712046 Shaanxi Institute of International Trade & Commerce, Xianyang 712046, China  
刘峰* LIU Feng 陕西步长制药有限公司,陕西 西安 710075 Shaanxi Buchang Pharmaceutical Co., Ltd., Xi'an 710075, China  
基金项目:陕西省重点研发计划项目(2020SF-313);陕西省重点产业创新链(群)-社会发展领域项目(2021ZDLSF04-09)
中文摘要:目的 建立电感耦合等离子体质谱法(ICP-MS)同时测定鲜地黄中Al、As、B、Ba、Ca、Cd等26种金属元素,分析和比较5种不同工艺加工鲜地黄样品中金属元素的组成差异,为完善鲜地黄的质量评价体系、筛选鲜地黄最佳加工工艺和产品开发提供参考。方法 制备了鲜地黄的喷雾干燥、冷冻干燥、微波干燥、真空干燥及烘箱干燥5种样品共15份,经微波消解后,采用ICP-MS同时测定5种不同加工工艺鲜地黄中26种金属元素,同时采用主成分分析(PCA)、正交偏最小二乘法-判别分析(OPLS-DA)及聚类分析(HCA)统计学方法进行数据处理和挖掘。结果 各元素线性关系良好(r>0.999),检测下限为0.004~12.493 μg·L–1,精密度符合规定(RSD<5%),各元素加样回收率为98.411%~100.541%,RSD为1.463%~3.505%;不同加工工艺鲜地黄样品含有丰富的金属元素,其中K、Na、Ca、Mg、Al、Fe、Zn 7种元素的含量均较高,但不同加工工艺鲜地黄样品金属元素含量差异较大;其中鲜地黄喷雾干燥后人体必须常量元素K、Na及人体所需的微量元素Fe、Zn、Se、Cu、Co、Mo等的含量均显著高于其他加工样品。PCA结果显示,15份不同加工工艺的地黄样品均能清晰地聚成5组;通过OPLS-DA提取出对不同加工工艺鲜地黄分组贡献率>1的5种金属元素,分别为K、Al、Na、Mg、Fe;HCA分析将15份样品分为2个组别,其中冷冻干燥和微波干燥为一组,烘箱干燥、喷雾干燥和真空干燥为一组,其第二组别下细分的小组中,喷雾干燥和真空干燥又聚为一组。结论 建立的方法简单、可靠、准确且灵敏度高,可用于鲜地黄不同加工样品中金属元素的测定;综合本研究结果及中药材加工技术的发展趋势和市场需求,喷雾干燥法能较好地保持鲜地黄的颜色及丰富的金属元素,且能实现生产化,喷雾干燥法可作为鲜地黄的加工方法。为完善鲜地黄质量控制体系及其开发利用提供一定的研究基础。
中文关键词:鲜地黄  不同加工工艺  金属元素  电感耦合等离子体质谱法  元素差异性比较
 
Establishment of an ICP-MS for Determination of 26 Metal Elements in Fresh Rehmanniae Radix and Comparison of Element Content between Different Processing Methods
Abstract:Objective The inductively coupled plasma-mass spectrometry (ICP-MS) method was established for the simultaneous determination of 26 metal elements such as Al, As, B, Ba, Ca, and Cd in fresh Rehmanniae Radix samples. Further, the content of metal elements in the fresh Rehmanniae Radix samples processed with five different methods was compared. This study aims to provide a scientific basis for improving the quality evaluation system and screening the optimum processing method of fresh Rehmanniae Radix products.Methods The fresh Rehmanniae Radix samples were prepared by spray drying, freeze drying, microwave drying, vacuum drying, and oven drying, respectively. After microwave digestion, ICP-MS was employed to simultaneously determine the content of 26 metal elements in the samples. The principal component analysis (PCA), orthogonal partial least squares-discriminant analysis (OPLS-DA), and hierarchical cluster analysis (HCA) were then performed.Results The established method showed good linear relationship of the metal element content (r>0.999), with the lower limits of detection of 0.004-12.493 μg·L–1 and the precision meeting the requirement (RSD<5%). The fresh Rehmanniae Radix samples processed with different methods contained rich metal elements, with high content of K, Na, Ca, Mg, Al, Fe, and Zn. The metal element content varied in the fresh Rehmanniae Radix samples processed with different methods. The content of the macroelements K and Na and the microelements Fe, Zn, Se, Cu, Co, and Mo essential for the human body were higher in the sample processed with spray drying than in the samples processed with other methods. PCA classified 15 batches of fresh Rehmanniae Radix samples processed with different methods into 5 groups. OPLS-DA extracted five metal elements with the contribution rate above 1 to the grouping of fresh Rehmanniae Radix samples processed with different methods, which were K, Al, Na, Mg, and Fe, respectively. HCA classified the 15 samples into two groups, including a group of samples processed with freeze drying and microwave drying and a group of samples processed with oven drying, spray drying, and vacuum drying, and the samples processed with spray drying and vacuum drying were clustered as a sub-group.Conclusion The method established in this study is simple, reliable, accurate, and sensitive and can be used for the determination of metal elements in the fresh Rehmanniae Radix samples processed with different methods. Considering the results of this study and the development trend and market demand of Chinese herbal medicine processing technology, we suggest that spray drying, which can maintain the color and rich metal elements and be industrialized, be selected as the optimum processing method of fresh Rehmanniae Radix. This study provides a research basis for improving the quality control system and the development and utilization of fresh Rehmanniae Radix.
keywords:fresh Rehmanniae Radix  different processing methods  metal elements  ICP-MS  element difference comparison
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