| 外源H2O2处理对药用大麻中大麻素含量的影响 |
| 投稿时间：2022-08-08 点此下载全文
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| 引用本文：李晓颖,姚杰,张利国,胡莹莹,何录文,孟祥才.外源H2O2处理对药用大麻中大麻素含量的影响[J].中国现代中药,2023,25(1):137-143 |
| DOI：10.13313/j.issn.1673-4890.20220808001 |
| 摘要点击次数: 363 |
| 全文下载次数: 58 |
| 作者中文名 | 作者英文名 | 单位中文名 | 单位英文名 | E-Mail |
| 李晓颖 |
LI Xiao-ying |
黑龙江中医药大学，黑龙江 哈尔滨 150040 |
Heilongjiang University of Chinese Medicine, Harbin 150040, China |
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| 姚杰 |
YAO Jie |
黑龙江中医药大学，黑龙江 哈尔滨 150040 |
Heilongjiang University of Chinese Medicine, Harbin 150040, China |
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| 张利国 |
ZHANG Li-guo |
黑龙江省农业科学院经济作物研究所，黑龙江 哈尔滨 150086 |
Cash Crops Institute of Heilongjiang Academy of Agricultural Sciences, Harbin 150086, China |
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| 胡莹莹 |
HU Ying-ying |
黑龙江省农业科学院经济作物研究所，黑龙江 哈尔滨 150086 |
Cash Crops Institute of Heilongjiang Academy of Agricultural Sciences, Harbin 150086, China |
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| 何录文 |
HE Lu-wen |
黑龙江中医药大学，黑龙江 哈尔滨 150040 |
Heilongjiang University of Chinese Medicine, Harbin 150040, China |
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| 孟祥才* |
MENG Xiang-cai |
黑龙江中医药大学，黑龙江 哈尔滨 150040 |
Heilongjiang University of Chinese Medicine, Harbin 150040, China |
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| 基金项目:国家自然科学基金联合基金项目（U20A20407） |
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| 中文摘要:目的 通过外源活性氧调节大麻的次生代谢，提高大麻二酚（CBD）含量，为优质药用大麻生产提供新的技术。方法 在大麻始果期采摘大麻植株顶端苞片，分别采用清水和20、200、2000 μmol·L–1过氧化氢（H2O2）对大麻苞片进行喷洒处理，连续4 d分析大麻体内活性氧含量、抗氧化酶活性、大麻素生物合成途径关键基因表达量及大麻素类成分含量的变化规律。结果 外源H2O2使大麻苞片中的H2O2、O2·–及丙二醛（MDA）含量均有所提升。20 μmol·L–1 H2O2处理使大麻超氧化物歧化酶（SOD）、过氧化氢酶（CAT）活力提升最大，在处理第1~2天达到峰值。过氧化物酶（POD）在H2O2处理第3天达到峰值。外源H2O2能够明显提升聚酮合酶、四氢大麻酚酸合成酶、大麻二酚酸合成酶的基因表达量。20 μmol·L–1 H2O2处理第1天大麻苞片中CBD含量比对照组提高了72.9%。结论 外源活性氧能够诱导出植物的逆境生理状态，20 μmol·L–1 H2O2处理能够极显著提高大麻CBD含量。 |
| 中文关键词:大麻 大麻素 过氧化氢 活性氧 |
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| Effect of Exogenous H2O2 Treatment on Cannabinoid Content in Medicinal Cannabis sativa |
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| Abstract:Objective To regulate the secondary metabolism of Cannabis sativa with exogenous reactive oxygen species, so as to improve the cannabidiol (CBD) content in this medicinal species, and to provide a new technique for the culture of quality C. sativa.Methods The bracts at the top of C. sativa in the initial fruiting stage were collected, which were classified into four groups and sprayed with clear water, 20 μmol·L–1 H2O2, 200 μmol·L–1 H2O2, and 2000 μmol·L–1 H2O2 solution, respectively, for 4 days. The changing rules of reactive oxygen species content, antioxidant enzyme activity, expression of genes involved in cannabinoid biosynthesis pathway, and cannabinoid content in C. sativa were analyzed.Results Exogenous H2O2 significantly increased the content of H2O2, O2·–, and malondialdehyde (MDA) in C. sativa bracts. The 20 μmol·L–1 H2O2 topped the treatments in improving the activity of superoxide dismutase (SOD) and catalase (CAT), which peaked on the 1st-2nd day of treatment. In this treatment, the activity of peroxidase (POD) peaked on the 3rd day. Exogenous H2O2 can significantly raise the expression of genes encoding polyketide synthase (Ols), tetrahydrocannabinolic acid (Thcas) synthase, and cannabidiolic acid (Cbdas) synthase. Compared with the control group, 20 μmol·L–1 H2O2 improved the CBD content in bracts of C. sativa by 72.9% on the first day.Conclusion Exogenous reactive oxygen species can induce the physiological state of plants in response to stress, and the spray with 20 μmol·L–1 H2O2 can significantly increase the CBD content of C. sativa . |
| keywords:Cannabis sativa L. cannabinoids H2O2 reactive oxygen species |
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