| 旋覆花-赭石药对酚酸类成分UPLC指纹图谱及多指标成分含量测定 |
| 投稿时间：2022-06-02 点此下载全文
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| 引用本文：陈巧华,邓怡芳,文珊,程钰洁,陈伟媚,钟霞,曾昭君,刘燎原.旋覆花-赭石药对酚酸类成分UPLC指纹图谱及多指标成分含量测定[J].中国现代中药,2023,25(1):157-164 |
| DOI：10.13313/j.issn.1673-4890.20220602005 |
| 摘要点击次数: 296 |
| 全文下载次数: 57 |
| 作者中文名 | 作者英文名 | 单位中文名 | 单位英文名 | E-Mail |
| 陈巧华 |
CHEN Qiao-hua |
广东一方制药有限公司/广东省中药配方颗粒企业重点实验室，广东 佛山 528244 |
Guangdong Yifang Pharmaceutical Co., Ltd./Guangdong Provincial Key Laboratory of Traditional Chinese Medicine Formula Granule, Foshan 528244, China |
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| 邓怡芳 |
DENG Yi-fang |
广东一方制药有限公司/广东省中药配方颗粒企业重点实验室，广东 佛山 528244 |
Guangdong Yifang Pharmaceutical Co., Ltd./Guangdong Provincial Key Laboratory of Traditional Chinese Medicine Formula Granule, Foshan 528244, China |
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| 文珊 |
WEN Shan |
广东一方制药有限公司/广东省中药配方颗粒企业重点实验室，广东 佛山 528244 |
Guangdong Yifang Pharmaceutical Co., Ltd./Guangdong Provincial Key Laboratory of Traditional Chinese Medicine Formula Granule, Foshan 528244, China |
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| 程钰洁 |
CHENG Yu-jie |
广东一方制药有限公司/广东省中药配方颗粒企业重点实验室，广东 佛山 528244 |
Guangdong Yifang Pharmaceutical Co., Ltd./Guangdong Provincial Key Laboratory of Traditional Chinese Medicine Formula Granule, Foshan 528244, China |
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| 陈伟媚 |
CHEN Wei-mei |
广东一方制药有限公司/广东省中药配方颗粒企业重点实验室，广东 佛山 528244 |
Guangdong Yifang Pharmaceutical Co., Ltd./Guangdong Provincial Key Laboratory of Traditional Chinese Medicine Formula Granule, Foshan 528244, China |
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| 钟霞 |
ZHONG Xia |
广东一方制药有限公司/广东省中药配方颗粒企业重点实验室，广东 佛山 528244 |
Guangdong Yifang Pharmaceutical Co., Ltd./Guangdong Provincial Key Laboratory of Traditional Chinese Medicine Formula Granule, Foshan 528244, China |
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| 曾昭君* |
ZENG Zhao-jun |
广东一方制药有限公司/广东省中药配方颗粒企业重点实验室，广东 佛山 528244 |
Guangdong Yifang Pharmaceutical Co., Ltd./Guangdong Provincial Key Laboratory of Traditional Chinese Medicine Formula Granule, Foshan 528244, China |
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| 刘燎原 |
LIU Liao-yuan |
广东一方制药有限公司/广东省中药配方颗粒企业重点实验室，广东 佛山 528244 |
Guangdong Yifang Pharmaceutical Co., Ltd./Guangdong Provincial Key Laboratory of Traditional Chinese Medicine Formula Granule, Foshan 528244, China |
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| 基金项目:工业和信息化部2019年产业技术基础公共服务平台项目（2019-00902-1-2） |
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| 中文摘要:目的 建立旋覆花-赭石药对超高效液相色谱法（UPLC）指纹图谱及多指标成分含量同时测定的方法。方法 采用Agilent ZORBAX RRHD StableBond C18色谱柱（100 mm×2.1 mm，1.8 μm），以乙腈-0.1%磷酸水溶液为流动相梯度洗脱，流速为0.3 mL·min–1，检测波长为327 nm，柱温为35 ℃，进样量为1 μL，建立旋覆花-赭石药对UPLC指纹图谱及同时测定新绿原酸、绿原酸、隐绿原酸、咖啡酸、异槲皮苷、异绿原酸B、1,5-二咖啡酰奎宁酸、异绿原酸C共8个指标成分含量的方法，并结合相似度评价、聚类分析（CA）及主成分分析（PCA）等化学模式识别方法对UPLC指纹图谱进行多元统计分析。结果 建立了旋覆花-赭石药对的UPLC指纹图谱，确定了16个共有峰，指认了其中8个成分。相似度评价结果表明，10批样品的相似度均大于0.990，CA及PCA均可将10批样品聚为3类。建立的含量测定方法稳定可靠，精密度、重复性、稳定性及加样回收率试验结果均良好。结论 建立的UPLC指纹图谱及同时测定8个指标成分含量的方法可用于旋覆花-赭石药对的质量控制。 |
| 中文关键词:旋覆花-赭石 药对 指纹图谱 化学模式识别 质量控制 |
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| Determination of Multiple Index Components and UPLC Fingerprint of Phenolic Acids in Inulae Flos-Haematitum Herb Pair |
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| Abstract:Objective To establish a method for simultaneously determining the UPLC fingerprint and the content of multiple index components in the Inulae Flos-Haematitum herb pair.Methods The gradient elution was performed in Agilent ZORBAX RRHD StableBond C18 column (100 mm×2.1 mm, 1.8 μm) with the mobile phase composed of acetonitrile and 0.1% phosphoric acid aqueous solution at the flow rate of 0.3 mL·min–1, the detection wavelength of 327 nm, the column temperature of 35 ℃, and the injection volume of 1 μL. The UPLC fingerprint of the herb pair was established. Meanwhile, the content of neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, caffeic acid, isoquercetin, isochlorogenic acid B, 1,5-dicaffeoylquinic acid, and isochlorogenic acid C were determined. Further, similarity evaluation, cluster analysis, and principal component analysis were performed to analyze the UPLC fingerprint.Results The UPLC fingerprint of the herb pair Inulae Flos-Haematitum was established, including 16 common peaks, among which 8 peaks were identified. The similarity of 10 batches of samples were greater than 0.990. The cluster analysis and principal component analysis divided the 10 batches of samples into three categories. The established content determination method was reliable, with good precision, repeatability, stability, and sample recovery.Conclusion The established method for simultaneously determining the UPLC fingerprint and 8 index components can be used for the quality control of Inulae Flos-Haematitum herb pair. |
| keywords:Inulae Flos-Haematitum herb pair fingerprint chemical pattern recognition quality control |
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