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作者中文名	作者英文名	单位中文名	单位英文名	E-Mail
何欣	HE Xin	中国医学科学院北京协和医学院药用植物研究所中草药物质基础与资源利用教育部重点实验室/濒危药材繁育国家工程实验室，北京 100193 中国医学科学院北京协和医学院药用植物研究所海南分所海南省南药资源保护与开发重点实验室/国家中医药管理局沉香可持续利用重点研究室，海南海口 570311	Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education & National Engineering Laboratory for Breeding of Endangered Medicinal Materials, Institute of Medicinal Plant Development, Chinese Academy of Medical Science & Peking Union Medical College, Beijing 100193, China Hainan Provincial Key Laboratory of Resources Conservation and Development of Southern Medicine & Key Laboratory of State Administration of Traditional Chinese Medicine for Agarwood Sustainable Utilization, Hainan Branch Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Haikou 570311, China
黄颖	HUANG Ying	中国医学科学院北京协和医学院药用植物研究所中草药物质基础与资源利用教育部重点实验室/濒危药材繁育国家工程实验室，北京 100193 中国医学科学院北京协和医学院药用植物研究所海南分所海南省南药资源保护与开发重点实验室/国家中医药管理局沉香可持续利用重点研究室，海南海口 570311	Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education & National Engineering Laboratory for Breeding of Endangered Medicinal Materials, Institute of Medicinal Plant Development, Chinese Academy of Medical Science & Peking Union Medical College, Beijing 100193, China Hainan Provincial Key Laboratory of Resources Conservation and Development of Southern Medicine & Key Laboratory of State Administration of Traditional Chinese Medicine for Agarwood Sustainable Utilization, Hainan Branch Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Haikou 570311, China
暴正瀚	BAO Zheng-han	中国医学科学院北京协和医学院药用植物研究所中草药物质基础与资源利用教育部重点实验室/濒危药材繁育国家工程实验室，北京 100193 中国医学科学院北京协和医学院药用植物研究所海南分所海南省南药资源保护与开发重点实验室/国家中医药管理局沉香可持续利用重点研究室，海南海口 570311	Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education & National Engineering Laboratory for Breeding of Endangered Medicinal Materials, Institute of Medicinal Plant Development, Chinese Academy of Medical Science & Peking Union Medical College, Beijing 100193, China Hainan Provincial Key Laboratory of Resources Conservation and Development of Southern Medicine & Key Laboratory of State Administration of Traditional Chinese Medicine for Agarwood Sustainable Utilization, Hainan Branch Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Haikou 570311, China
孟慧	MENG Hui	中国医学科学院北京协和医学院药用植物研究所中草药物质基础与资源利用教育部重点实验室/濒危药材繁育国家工程实验室，北京 100193 中国医学科学院北京协和医学院药用植物研究所海南分所海南省南药资源保护与开发重点实验室/国家中医药管理局沉香可持续利用重点研究室，海南海口 570311	Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education & National Engineering Laboratory for Breeding of Endangered Medicinal Materials, Institute of Medicinal Plant Development, Chinese Academy of Medical Science & Peking Union Medical College, Beijing 100193, China Hainan Provincial Key Laboratory of Resources Conservation and Development of Southern Medicine & Key Laboratory of State Administration of Traditional Chinese Medicine for Agarwood Sustainable Utilization, Hainan Branch Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Haikou 570311, China
李浩凌	LI Hao-ling	中国医学科学院北京协和医学院药用植物研究所海南分所海南省南药资源保护与开发重点实验室/国家中医药管理局沉香可持续利用重点研究室，海南海口 570311	Hainan Provincial Key Laboratory of Resources Conservation and Development of Southern Medicine & Key Laboratory of State Administration of Traditional Chinese Medicine for Agarwood Sustainable Utilization, Hainan Branch Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Haikou 570311, China
魏建和	WEI Jian-he	中国医学科学院北京协和医学院药用植物研究所中草药物质基础与资源利用教育部重点实验室/濒危药材繁育国家工程实验室，北京 100193 中国医学科学院北京协和医学院药用植物研究所海南分所海南省南药资源保护与开发重点实验室/国家中医药管理局沉香可持续利用重点研究室，海南海口 570311	Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education & National Engineering Laboratory for Breeding of Endangered Medicinal Materials, Institute of Medicinal Plant Development, Chinese Academy of Medical Science & Peking Union Medical College, Beijing 100193, China Hainan Provincial Key Laboratory of Resources Conservation and Development of Southern Medicine & Key Laboratory of State Administration of Traditional Chinese Medicine for Agarwood Sustainable Utilization, Hainan Branch Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Haikou 570311, China
杨云*	YANG Yun	中国医学科学院北京协和医学院药用植物研究所中草药物质基础与资源利用教育部重点实验室/濒危药材繁育国家工程实验室，北京 100193 中国医学科学院北京协和医学院药用植物研究所海南分所海南省南药资源保护与开发重点实验室/国家中医药管理局沉香可持续利用重点研究室，海南海口 570311	Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education & National Engineering Laboratory for Breeding of Endangered Medicinal Materials, Institute of Medicinal Plant Development, Chinese Academy of Medical Science & Peking Union Medical College, Beijing 100193, China Hainan Provincial Key Laboratory of Resources Conservation and Development of Southern Medicine & Key Laboratory of State Administration of Traditional Chinese Medicine for Agarwood Sustainable Utilization, Hainan Branch Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Haikou 570311, China

基金项目:国家重点研发计划项目（2018YFC1706401）；中国医学科学院医学与健康科技创新工程项目（2021-I2M-1-032）

中文摘要:目的评价高压输液法用于通体结香技术的可行性。方法分别在常压和10、20、30 MPa压力下采用通体结香技术处理白木香，记录输液时间，筛选最佳高压输液压力；在晴天、夜晚、雨天和低温4种不同结香环境条件下采用常压和高压输液处理，记录输液时间，根据《中华人民共和国药典》（以下简称《中国药典》）2020年版沉香质量标准，对处理6个月的沉香样品进行性状、显微观察，浸出物、结香率、沉香四醇质量分数、特征图谱分析。结果 20 MPa为高压输液法通体结香技术最佳输液压力，可显著缩短通体结香技术不同结香环境条件下的输液时间，输液效率提高129%~497%。20 MPa压力下通体香品质均符合《中国药典》2020年版标准，沉香层呈棕黑色，环形沉香层外有棕色星点状沉香外溢现象；平均结香率、浸出物平均质量分数和沉香四醇平均质量分数分别为11.69%、15.14%和0.45%，与常压差异无统计学意义；薄层色谱和特征图谱检测结果与对照药材一致。结论高压输液法通体结香技术能明显提高该技术的输液效率，既不受天气和气候影响，也不降低结香品质。该技术具有较强的可行性，可在实际生产中推广应用。

中文关键词:沉香通体结香技术高压输液技术升级

High-pressure Infusion Method in Whole-tree Agarwood-inducing Technique: Inducing Effect and Agarwood Quality

Abstract:Objective To evaluate the feasibility of high-pressure infusion in the whole-tree agarwood-inducing technique (Agar-WIT).Methods Agar-WIT was employed to treat Aquilaria sinensis under normal pressure, 10 MPa, 20 MPa, and 30 MPa, respectively. The infusion time was recorded, and the optimal infusion pressure was selected. Under four different environmental conditions of sunny day, night, rainy day, and low temperature, normal-pressure infusion and high-pressure infusion were used to induce agarwood, and the infusion time was recorded. According to the standards in the Chinese Pharmacopeia (2020 edition), the agarwood samples after 6 months of treatment with Agar-WIT were studied in terms of appearance, microscopic characteristics, extract content, agarwood area ratio, agarotetrol content, and characteristic chromatogram.Results The optimal pressure for the high-pressure infusion was 20 MPa, which significantly shortened the infusion time under different production conditions and increased the infusion efficiency by 129%-497%. The agarwood produced under 20 MPa showed the quality conforming to the standards in the Chinese Pharmacopoeia (2020 edition), with a brownish black agarwood layer and a brown star-shaped agarwood overflow outside the annular agarwood layer. The average agarwood area ratio, the average content of extract, and the average content of agarotetrol were 11.69%, 15.14%, and 0.45%, respectively, which had no significant differences from those of the agarwood produced under 1 MPa. The thin-layer chromatogram and characteristic chromatogram of the agarwood produced were consistent with those of the reference medicinal material.Conclusion The high-pressure infusion method characterized by high infusion efficiency is not susceptible to weather or climate and does not compromise the agarwood quality. This method has strong feasibility and can be popularized in production.

keywords:agarwood whole-tree agarwood-inducing technique (Agar-WIT) high-pressure infusion technology upgrading

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