多年生乌拉尔甘草复合丛枝菌根真菌接种效应研究
投稿时间:2023-02-09     点此下载全文
引用本文:汪金叶,王升,张燕,张乃嘦,杨明,小杉実加,孙楷,郭兰萍.多年生乌拉尔甘草复合丛枝菌根真菌接种效应研究[J].中国现代中药,2023,25(4):839-846
DOI:10.13313/j.issn.1673-4890.20230209004
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作者中文名作者英文名单位中文名单位英文名E-Mail
汪金叶 WANG Jin-ye 安徽中医药大学 药学院,安徽 合肥 230012
中国中医科学院 中药资源中心 道地药材国家重点实验室培育基地,北京 100700
School of Pharmacy, Anhui University of Chinese Medicine, Hefei 230012, China
State Key Laboratory Base of Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China
 
王升 WANG Sheng 中国中医科学院 中药资源中心 道地药材国家重点实验室培育基地,北京 100700 State Key Laboratory Base of Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China  
张燕 ZHANG Yan 中国中医科学院 中药资源中心 道地药材国家重点实验室培育基地,北京 100700 State Key Laboratory Base of Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China  
张乃嘦 ZHANG Nai-wu 盛实百草药业有限公司, 天津 300301 China Medico Corporation, Tianjin 300301, China  
杨明 YANG Ming 盛实百草药业有限公司, 天津 300301 China Medico Corporation, Tianjin 300301, China  
小杉実加 KOSUGI Mika 株式会社津村, 日本 300-1192 Tsumura & Co., 300-1192, Japan  
孙楷* SUN Kai 中国中医科学院 中药资源中心 道地药材国家重点实验室培育基地,北京 100700 State Key Laboratory Base of Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China  
郭兰萍 GUO Lan-ping 中国中医科学院 中药资源中心 道地药材国家重点实验室培育基地,北京 100700 State Key Laboratory Base of Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China  
基金项目:国家自然科学基金重大项目(81891014);中国中医科学院科技创新工程项目(CI2021A03905,CI2021B013);中国中医科学院优秀青年科技人才培养专项(ZZ13-YQ-094)
中文摘要:目的 探究2种丛枝菌根真菌复合与独立接种对田间栽培多年生乌拉尔甘草生长与有效成分积累的长短期效应。方法 选取摩西斗管囊霉Funneliformis mosseae与根内根孢囊霉Rhiaophagus intraradices 2种丛枝菌根真菌菌种,在大田条件下对乌拉尔甘草进行单菌种与复合菌种接种处理,分别于栽培的第1年和第2年采集乌拉尔甘草样品,分析其形态指标和有效成分甘草酸、甘草苷含量。结果 田间试验结果表明,复合接种和摩西斗管囊霉单接种均对乌拉尔甘草中甘草酸与甘草苷含量有提升作用,其中复合接种提升效果最为显著,其次为摩西斗管囊霉单接种,根内根孢囊霉单接种处理无显著提升作用。接种处理组乌拉尔甘草种苗的生长指标与对照组差异无统计学意义。接种丛枝菌根真菌显著提高了甘草苷含量与甘草酸含量的比值,接种组甘草苷、甘草酸的积累速率呈显著正相关且与对照组有明显差异。结论 揭示了大田条件下复合丛枝菌根真菌接种的长期效应,发现摩西斗管囊霉与根内根孢囊霉复合接种可以提高大田栽培乌拉尔甘草的品质,并对甘草苷与甘草酸的含量比例有调节作用,为人工栽培乌拉尔甘草过程中丛枝菌根真菌利用提供参考。
中文关键词:乌拉尔甘草  摩西斗管囊霉  根内根孢囊霉  甘草酸  甘草苷
 
Effects of Co-inoculation with Arbuscular Mycorrhizal Fungi on Perennial Glycyrrhiza uralensis
Abstract:Objective To explore the long-term and short-term effects of co-inoculation and single inoculation with two arbuscular mycorrhizal fungi on the growth and accumulation of active components of perennial Glycyrrhiza uralensis. in field cultivation.Methods G. uralensis was inoculated individually or in combination with Funneliformis mosseae and Rhiaophagus intraradices under field conditions. G. uralensis samples were collected in the first and second years of cultivation to analyze the morphological indexes and the content of glycyrrhizic acid and liquiritin.Results The results of the field experiment showed that both co-inoculation and single inoculation with F. mosseae could increase the glycyrrhizic acid and liquiritin content in G. uralensis, in which the co-inoculation had the most significant effect, followed by single inoculation with F. mosseae. The single inoculation with R. intraradices had no significant effect. There was no significant difference in the growth indexes between the inoculation groups and the control group. The inoculation with arbuscular mycorrhizal fungi significantly increased the content ratio of liquiritin to glycyrrhizic acid, and the accumulation rates of liquiritin and glycyrrhizic acid in the inoculation groups showed significantly positive correlations and were different from that in the control group.Conclusion This study revealed the long-term effects of arbuscular mycorrhizal fungi co-inoculation under filed conditions, and found that the co-inoculation with F. mosseae and R. intraradices could improve the quality of G. uralensis cultivated in the field and regulate the content ratio of liquiritin to glycyrrhizic acid, which is expected to provide a reference for the utilization of arbuscular mycorrhizal fungi in the artificial cultivation of G. uralensis.
keywords:Glycyrrhiza uralensis Fisch.  Funneliformis mosseae  Rhiaophagus intraradices  glycyrrhizic acid  liquiritin
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