| 华蟾素对乳腺癌MDA-MB-231细胞增殖迁移凋亡的影响 |
| 投稿时间：2017-09-07 点此下载全文
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| 引用本文：郑迪,代国,张政佩,陶春杰,余铃,郭卫春.华蟾素对乳腺癌MDA-MB-231细胞增殖迁移凋亡的影响[J].中国现代中药,2018,20(4):409-414 |
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| 基金项目:国家自然科学基金项目（81341078） |
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| 中文摘要:目的：研究华蟾素对MDA-MB-231细胞增殖、迁移和凋亡的影响，并探讨其可能的作用机制。方法：体外培养三阴性乳腺癌细胞MDA-MB-231，用倒置显微镜观察不同浓度华蟾素对MDA-MB-231细胞形态的影响；CCK-8实验检测华蟾素对MDA-MB-231细胞增殖的抑制作用；划痕实验检测华蟾素对MDA-MB-231细胞迁移能力的抑制作用；FITC/PI双染流式细胞术检测不同浓度华蟾素对MDA-MB-231细胞凋亡率的影响；Western Blot检测凋亡相关蛋白Bax、Bcl-2、Cleaved capsase-3、Cleaved caspase-9和PTHrP的表达。结果：CCK-8结果显示华蟾素可以时间、浓度依赖性地抑制MDA-MB-231细胞增殖；划痕实验结果表明华蟾素可抑制MDA-MB-231细胞迁移；流式细胞术结果表明华蟾素可浓度依赖性地诱导MDA-MB-231细胞凋亡；Western Blot结果表明，华蟾素可上调MDA-MB-231细胞中促凋亡蛋白Bax、Cleaved capsase-3、Cleaved caspase-9，下调抑凋亡蛋白Bcl-2。此外，华蟾素还可抑制MDA-MB-231细胞中PTHrP的表达。结论：华蟾素通过线粒体途径诱导MDA-MB-231细胞凋亡可能是其抗三阴性乳腺癌的机制；华蟾素通过下调乳腺癌MDA-MB-231细胞中PTHrP进而抑制溶骨性骨转移的发生，有待于后续的动物实验来进行验证。 |
| 中文关键词:华蟾素 MDA-MB-231细胞 凋亡 PTHrP |
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| Effect of Cinobufacini on MDA-MB-231 Cells Proliferation，Migration and Apoptosis |
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| Abstract:Objective：To investigate the effect of cinobufacini on MDA-MB-231 cells proliferation，migration and apoptosis，and detect the underlying mechanism.Methods：MDA-MB-231 cells were treated with different concentrations of cinobufacini，the morphological changes of MDA-MB-231 cells were observed under inverted microscope.CCK-8 assay was used to detect the effect of cinobufacini on MDA-MB-231 cell proliferation.Scratches test was employed to investigate cell migration in MDA-MB-231 cells and cell apoptosis was analyzed by flow cytometry with the staining of Annexin V-FITC/PI.Western Blot was employed to detect the expression level of Bax，Bcl-2，Cleaved capsase-3，Cleaved caspase-9 and PTHrP.Results：CCK-8 results showed that cinibufacini could inhibit the proliferation of MDA-MB-231 cells in a concentration and time-dependent manner.Scratches test confirmed that cinibufacini treatment reduced the migration ability of MDA-MB-231 cells.The results of flow cytometry suggested that cinibufacini could induce apoptosis in MDA-MB-231 cells in a concentration dependent manner.The expression levels of Bax，Cleaved capsase-3，Cleaved caspase-9 were increased，whereas the expression levels of Bcl-2 and PTHrP were decreased with the treatment of cinobufacini.Conclusion：Cinobufacini could induce apoptosis through the mitochondrial apoptotic pathway to exert anti-tumor effect on MDA-MB-231 cells.The effect of cinobufacini on breast cancer osteolytic bone metastasis needs to be further studied in vivo . |
| keywords:Cinobufacini MDA-MB-231 cell apoptosis PTHrP |
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