| 6种中药材黄曲霉毒素B1快速检测方法研究 |
| 投稿时间：2018-01-04 点此下载全文
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| 引用本文：李细芬,毛雯雯,张雅琴,冯红,许宏亮,李彦川,蔡莹瀛,张兰兰.6种中药材黄曲霉毒素B1快速检测方法研究[J].中国现代中药,2018,20(8):968-974 |
| DOI： |
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| 作者中文名 | 作者英文名 | 单位中文名 | 单位英文名 | E-Mail |
| 李细芬 |
LI Xi-fen |
数字本草检测有限公司，天津300400 |
Digital Materia Medica Festing Technology Co.,Ltd.，Tianjin 300400,China |
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| 毛雯雯 |
MAO Wen-wen |
数字本草检测有限公司，天津300400 |
Digital Materia Medica Festing Technology Co.,Ltd.，Tianjin 300400,China |
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| 张雅琴 |
ZHANG Ya-qin |
数字本草检测有限公司，天津300400 |
Digital Materia Medica Festing Technology Co.,Ltd.，Tianjin 300400,China |
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| 冯红 |
FENG Hong |
数字本草检测有限公司，天津300400 |
Digital Materia Medica Festing Technology Co.,Ltd.，Tianjin 300400,China |
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| 许宏亮 |
XU Hong-liang |
数字本草检测有限公司，天津300400 |
Digital Materia Medica Festing Technology Co.,Ltd.，Tianjin 300400,China |
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| 李彦川 |
LI Yan-chuan |
数字本草检测有限公司，天津300400 |
Digital Materia Medica Festing Technology Co.,Ltd.，Tianjin 300400,China |
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| 蔡莹瀛 |
CAI Ying-ying |
数字本草检测有限公司，天津300400 |
Digital Materia Medica Festing Technology Co.,Ltd.，Tianjin 300400,China |
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| 张兰兰 |
ZHANG Lan-lan |
数字本草检测有限公司，天津300400 |
Digital Materia Medica Festing Technology Co.,Ltd.，Tianjin 300400,China |
张兰兰，高级研究员，研究方向：中药材资源与检测，E-Mail：zhangll2@tasly.com |
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| 基金项目:国家中医药现代化研究重点专项，中药材外源性有毒有害物质检测和控制标准研究，2017YFC1700806；天津市科技计划项目，中药材质量控制快速检测技术研发，17YFCZZC00350。 |
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| 中文摘要:目的：建立中药材黄曲霉毒素B1胶体金快检方法。方法：对酸枣仁、莲子、薏苡仁、槟榔、决明子和远志6种中药材的前处理方法进行研究，使胶体金法检测能够检测6种中药材中黄曲霉毒素B1；同时建立高效液相色谱法为标准方法，对快检方法的准确性进行验证。结果：胶体金快检方法检测6种中药材黄曲霉毒素B1灵敏度为5 μg·kg-1，重复性和特异性良好；两种方法检测6种中药材样品共计111批次，结果表明快检方法的判断准确性与液相方法相比达到98.2%。结论：快检方法对样品的结果判断与液相色谱法高度一致，胶体金快检方法简单、快速、准确，可用于酸枣仁等6种中药材中黄曲霉毒素B1筛查。 |
| 中文关键词:中药材 胶体金 快检 黄曲霉毒素B1 高效液相色谱法 |
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| Study on Rapid Detection Method for Aflatoxin B1 in Six Kinds of Traditional Chinese Medicine |
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| Abstract:Objective：To establish a method for the rapid detection of aflatoxinB1 in traditional Chinese medicines（TCM），the colloidal gold immunochromatography assay（GICA） was applied.Methods：The pretreatment methods of six kinds of TCM，including Ziziphi Spinosae Semen，Nelumbinis Semen，Coicis Semen，Arecae Semen，Cassiae Semen and Polygalae Radix，were improved.And a standard HPLC method was established to confirm the accuracy of GICA.Results：The developed methods were proved to possess high specificity and reproducibility，and the sensitivity was 5 μg·kg-1 111 batches samples of six kinds of TCM were used in the detection of aflatoxin B1 by GICA and HPLC methods.The total accuracy rate of GICA was 98.2%.Conclusion：The results indicated that GICA and HPLC methods showed highly conformance in the detection of aflatoxin B1.Therefore，GICA could be employed to screen aflatoxin B1 in the six kinds of TCM rapidly. |
| keywords:traditional Chinese medicine colloidal gold method rapid determine aflatoxin B1 HPLC |
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