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傣药人面果叶中总黄酮含量测定方法研究

投稿时间：2018-03-14 点此下载全文

引用本文：刘慧,钟芳芳,谭雪,卢青秀,杨光忠,陈玉.傣药人面果叶中总黄酮含量测定方法研究[J].中国现代中药,2018,20(9):1105-1109

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作者中文名	作者英文名	单位中文名	单位英文名	E-Mail
刘慧	LIU Hui	中南民族大学化学与材料科学学院，湖北武汉430074	College of Chemistry and Materials Science，South Central University for Nationalities，Wuhan 430074，China
钟芳芳	ZHONG Fang-fang	温州市洞头区产品质量监督检验所，浙江温州325000	Wenzhou Dongtou Product Quality Supervision and Testing Institute，Wenzhou 325000，China
谭雪	TAN Xue	中南民族大学民族药学国家级实验教学示范中心，湖北武汉430074	National Demonstration Center for Experimental Ethnopharmacology Education，South-Central Universityfor Nationalities，Wuhan 430074，China
卢青秀	LU Qing-xiu	中南民族大学化学与材料科学学院，湖北武汉430074	College of Chemistry and Materials Science，South Central University for Nationalities，Wuhan 430074，China
杨光忠	YANG Guang-zhong	中南民族大学民族药学国家级实验教学示范中心，湖北武汉430074	National Demonstration Center for Experimental Ethnopharmacology Education，South-Central Universityfor Nationalities，Wuhan 430074，China
陈玉	CHEN Yu	中南民族大学化学与材料科学学院，湖北武汉430074	College of Chemistry and Materials Science，South Central University for Nationalities，Wuhan 430074，China	陈玉，教授，研究方向：天然药物化学；Tel：（027）67842752，E-mail：chenyuwh888@126.com

基金项目:国家自然科学基金资助项目(31370379)；国家重大新药创制科技重大专项(2017Z09301060)

中文摘要:目的：建立人面果叶总黄酮含量测定的方法。方法：比较NaNO2-Al（NO3）3-NaOH和AlCl3-CH3COONa/CH3COOH 两种显色方法测定人面果叶中总黄酮含量的差异。结果：用AlCl3-CH3COONa/ CH3COOH显色的方法，供试品和对照品Fukugetin在415 nm处显示有较强和稳定的吸收。结论：采用 AlCl3-CH3COONa/CH3COOH比色法测定人面果叶总黄酮的含量，并建立了该方法测定人面果叶总黄酮含量的最优条件。

中文关键词:人面果叶总黄酮含量测定紫外-可见分光光度法

Determination of Total Flavonoids from Leaves of Garcinia xanthochymus

Abstract:Objective：To establish a method for determination of total flavonoids from the leaves of Garcinia xanthochymus.Methods：NaNO2-Al（NO3）3-NaOH and AlCl3-CH3COONa/CH3COOH colorimetry methods were compared to determine the total flavonoids from the leaves of G.xanthochymus.Results：Both the test sample and Fukugetin showed strong and stable absorptions at 415 nm by AlCl3-CH3COONa/CH3COOH colorimetry.Conclusion：It is reasonable to determine the total flavonoids by using AlCl3-CH3COONa/CH3COOH colorimetry，the optimum condition of this method was selected.

keywords:Garcinia xanthochymus total flavonoids content determination UV-VIS spectrophotometry

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