| 复合酶法优化提取银杏叶总黄酮的工艺研究 |
| 投稿时间：2018-03-09 点此下载全文
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| 引用本文：李凤艳,王凯,朱鹏,孙国平.复合酶法优化提取银杏叶总黄酮的工艺研究[J].中国现代中药,2018,20(9):1142-1145 |
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| 基金项目:坪山新区卫生系统科研资助项目（201610）） |
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| 中文摘要:目的：研究纤维素酶/果胶酶/半纤维素酶复合酶酶解提取银杏叶中黄酮类化合物的最佳工艺条件。方法：以黄酮得率为考察指标，通过单因素试验及正交试验确定最佳提取工艺。结果：复合酶法提取银杏叶总黄酮的最佳工艺条件为酶用量0.4%，酶解温度40 ℃，酶解时间120 min，pH 5.5，纤维素酶/果胶酶/半纤维素酶的配比2∶3∶1时对银杏叶的细胞组织具有较强的破壁力；与不加酶相比，总黄酮提取率提高了36.6%。结论：采用复合酶法，可以降低酶的使用量，提取条件温和，提高了总黄酮提取率。 |
| 中文关键词:银杏叶 复合酶 总黄酮 优化 提取率 |
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| Optimization of Flavonoids Extraction from Ginkgo Biloba Leaves by Compound Enzymatic Method |
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| Abstract:Objective：To screen the optimum process conditions of the cellulose enzyme/pectin enzyme/hemicellulase composite enzyme for extracting flavonoids from ginkgo biloba.Methods：The flavonoid yield was selected as the examining index，the best extraction process was determined through the single factor experiment and orthogonal experiment.Results：The results showed that the temperature of enzymatic hydrolysis was 40 ℃，pH=5.5，consumption of enzyme dosage was 0.4%，the time of enzymatic hydrolysis was 120 min，ratio of cellulose enzyme/pectin enzyme/hemicellulase was 2：3：1.On this condition，the extraction yield of flavonoids from G.biloba leaves was increased by 36.6% compared with the non-using cellulase-assistant technology.Conclusion：Compound enzymatic method can reduce the usage of enzyme，improve the flavonoids extraction yield with extraction of mild conditions. |
| keywords:Ginkgobiloba leaves compound enzyme flavonoids optimization extraction yield |
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