人参黑斑病低毒化学杀菌剂的室内筛选
投稿时间:2018-07-06     点此下载全文
引用本文:李勇,杨小芳,张晓云,王蓉,丁万隆.人参黑斑病低毒化学杀菌剂的室内筛选[J].中国现代中药,2019,21(1):82-84
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作者中文名作者英文名单位中文名单位英文名E-Mail
李勇 LI Yong 中国医学科学院 北京协和医学院 药用植物研究所,北京100193 Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences & Peking Union Medical College,Beijing 100193,China  
杨小芳 YANG Xiao-fang 中国中医科学院 中药研究所,北京100700 Institute of Chinese Materia Medica,China Academy of Chinese Medical Science,Beijing 100700,China  
张晓云 ZHANG Xiao-yun 中国医学科学院 北京协和医学院 药用植物研究所,北京100193 Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences & Peking Union Medical College,Beijing 100193,China  
王蓉 WANG Rong 中国医学科学院 北京协和医学院 药用植物研究所,北京100193 Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences & Peking Union Medical College,Beijing 100193,China  
丁万隆 DING Wan-long 中国医学科学院 北京协和医学院 药用植物研究所,北京100193 Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences & Peking Union Medical College,Beijing 100193,China 丁万隆,研究员,研究方向:药用植物病害防治研究;E-mail:wlding@implad.ac.cn 
基金项目:中国医学科学院医学与健康科技创新工程项目(2016-I2M-3-017);国家中药标准化项目(ZYBZH-C-JL-27)
中文摘要:目的:室内筛选对人参黑斑菌有理想抑菌活性的低毒化学杀菌剂,为人参黑斑病防控提供参考依据。方法:采用平板培养法结合抑菌率及毒力测定,明确不同杀菌剂的抑菌效果。结果:新益甲托、金万霉灵和多菌灵对人参黑斑菌的毒力较好,EC50值分别为199.5、181.97和96.83 μg·mL-1;其次为代森锰锌、阿米西达和世高,EC50值分别为2041.7、3162.3和1258.9 μg·mL-1;其余杀菌剂的EC50值均偏高。结论:新益甲托对人参黑斑菌有较好的防病潜力,在确保病害防治效果及农药残留达标的前提下,可考虑将其用于人参黑斑病的田间防治。
中文关键词:人参  黑斑病  化学杀菌剂  室内筛选  毒力测定
 
Screening of Low Toxicity Chemical Fungicides for Control of Ginseng Black Spot Disease Caused by Alternaria panax
Abstract:Objective:To screen low mammalian-toxic pesticides in the library,and provide reference for the control of the leaf and stem blight disease caused by Alternaria panax Whetz in Panax ginseng.Methods:Plate-culturing method combined with the inhibition rate and toxicities were used to examine the efficiency of fungicides tested.Results:New Tono,golden mycophenolate and carbendazol existed the highest toxicity against Alternaria panax,and their EC50 values were 199.5 μg·mL-1,181.97 μg·mL-1 and 96.83 μg·mL-1,respectively.Then were Mancozeb,Amistar,and score with the EC50 values of 2041.7 μg·mL-1,3162.3 μg·mL-1 and 1258.9 μg·mL-1,respectively.The EC50 values of the other fungicides were higher.Conclusion:New Tono showed good disease controlling potential against Alternaria panax,which can be used for the control of ginseng leaf and stem blight disease in the field after ensure the disease controlling efficacy and pesticide residue.
keywords:Panax ginseng  Alternaria panax  fungicide  laboratory screening  toxicity test
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