| 响应面法优化黄芩茎叶对照提取物纯化工艺及其特征图谱研究 |
| 投稿时间：2019-03-22 点此下载全文
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| 引用本文：张雅蓉,徐犇,李纳,刘吉爽,徐文慧,陈新.响应面法优化黄芩茎叶对照提取物纯化工艺及其特征图谱研究[J].中国现代中药,2019,21(5):654-661 |
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| 基金项目:吉林省科技攻关计划（20150201007YY） |
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| 中文摘要:目的：采用响应面法（Box-Behnken）优化黄芩茎叶对照提取物的纯化工艺，并建立其特征图谱。方法：以黄芩茎叶总黄酮含量为评价指标，单因素试验结合响应面法分析，建立数学模型，优选对照提取物纯化工艺参数，并对制备的黄芩茎叶对照提取物采用高效液相色谱法进行特征图谱研究。结果：采用Design-Expert 10.0.3软件得到黄芩茎叶总黄酮得率与各因素变量的二次多元回归方程为：Y=65.58+1.68A+0.84B+1.55C+0.44D-0.16AB-1.12AC-0.58AD+0.62BC-0.067BD+0.30CD+0.52A2-1.46B2-1.25C2-1.47D2（P＜0.05），确定纯化工艺为乙醇浓度为80%，上样流速为1.5 mL·min-1，洗脱体积为5 BV，洗脱流速为4 mL·min-1。确定11批黄芩茎叶对照提取物高效液相特征图谱有7个共有峰，样品与对照特征图谱的相似度在0.957～0.989。结论：本实验优选的对照提取物纯化工艺稳定、可控，所建立的高效液相特征图谱可为黄芩茎叶对照提取物的研究及应用提供参考。 |
| 中文关键词:黄芩茎叶 对照提取物 响应面法 特征图谱 总黄酮 |
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| Response Surface Methodology for Optimizing Purification Technology and Characteristic Map of Control Extracts from Stems and Leaves of Scutellaria baicalensis |
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| Abstract:Objective：To optimize the purification process of the control extract from the stems and leaves of Scutellaria baicalensis by response surface methodology（Box-Behnken）and establish its characteristic map.Methods：The content of total flavonoids in the stems and leaves of S.baicalensis was taken as the evaluation index，the single factor experiment combined with response surface methodology was used to establish a mathematical model and optimize the purification process parameters of the crude extract，and the characteristic chromatogram of the extract was studied by HPLC.Results：The quadratic multiple regression equation of total flavonoids yield from stems and leaves of S.baicalensis with design-Expert 10.0.3 software was Y=65.58+1.68A+0.84B+1.55C+0.44D-0.16AB-1.12AC-0.58AD+0.62BC-0.067BD+0.30CD+0.52A2-1.46B2-1.25C2-1.47D2（P＜0.05）.The purification condition was determined to be 80% ethanol concentration.Sample flow rate was 1.5 mL·min-1.The elution volume was 5 BV.The elution flow rate was 4 mL·min-1.The characteristic chromatograms of 11 batches of control extracts from stems and leaves of S.baicalensis showed that there were 7 common peaks，ranging from 0.957-0.989.Conclusion：The purification process of the optimized control extract is stable and controllable.The established characteristic chromatogram of HPLC can provide theoretical reference for the research and application of the control extract from the stems and leaves of S.baicalensis. |
| keywords:Scutellaria baicalensis stem and leaf controlled extract response surface methodology characteristic map total flavonoids |
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