亲水色谱-超高效液相-串联质谱法测定滇橄榄中水解氨基酸研究
投稿时间:2019-03-26     点此下载全文
引用本文:王荌,车彦云,夏杰,李松梅,仇嘉,赵毅.亲水色谱-超高效液相-串联质谱法测定滇橄榄中水解氨基酸研究[J].中国现代中药,2020,22(1):47-52
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作者中文名作者英文名单位中文名单位英文名E-Mail
王荌 WANG An 云南省中医医院 科研部,云南昆明650021 Department of Research,Yunnan Provincial Hospital of Traditional Chinese Medicine,Kunming 650021,China  
车彦云 CHE Yan-yun 云南中医药大学,云南昆明650500 Yunnan University of Traditional Chinese Medicine,Kunming 650500,China  
夏杰 XIA Jie 云南省中医医院 科研部,云南昆明650021 Department of Research,Yunnan Provincial Hospital of Traditional Chinese Medicine,Kunming 650021,China  
李松梅 LI Song-mei 云南省中医医院 科研部,云南昆明650021 Department of Research,Yunnan Provincial Hospital of Traditional Chinese Medicine,Kunming 650021,China  
仇嘉 QIU Jia 云南省中医医院 科研部,云南昆明650021 Department of Research,Yunnan Provincial Hospital of Traditional Chinese Medicine,Kunming 650021,China  
赵毅 ZHAO Yi 云南中医药大学,云南昆明650500 Yunnan University of Traditional Chinese Medicine,Kunming 650500,China 赵毅,正高级工程师,研究方向:药物制剂及药物健康产品研发;Tel:(0871)65918210,E-mail:298007310@qq.com 
基金项目:云南中医药特色健康产品协同创新中心;云南中医药大学应用基础研究联合专项资金项目2019FF002(-012)
中文摘要:目的:建立滇橄榄中的水解氨基酸的亲水色谱-超高效液相-串联质谱法测定方法。方法:采用盐酸水解滇橄榄中的17种氨基酸,DE-52纤维素除氯离子后,利用亲水色谱柱,结合超高效液相色谱-串联质谱仪,采用正离子模式,多反应监测(MRM),外标法定量,测定滇橄榄中17种水解氨基酸。结果:DE-52纤维素能够有效去除水解溶液中的氯离子,亲水色谱柱对于亮氨酸和异亮氨酸等具有较好的分离效果,在0.000 3~2.0 μg·mL-1质量浓度范围内,17种氨基酸的线性关系较好,检出限为0.015~13.0 mg·kg-1,回收率为73.8%~98.8%,RSD为3.25%~7.79%。结论:该方法重复性好、灵敏度高,适合滇橄榄中17种水解氨基酸的测定。
中文关键词:亲水色谱  超高效液相色谱-串联质谱  滇橄榄(余甘子)  水解氨基酸
 
Determination of Hydrolyzed Amino Acids by Hydrophilic Chromatography-Ultra high performance liquid-tandem mass spectrometry in Phyllanthus emblica Linn.
Abstract:Objective:To establis a method for the determination of hydrolyzed amino acids in the Phyllanthus emblica Linn. by hydrophilic chromatography-ultra-high performance liquid chromatography-tandem mass spectrometry. Methods:Hydrochloric acid was used to hydrolyze 17 amino acids in the P.emblica Linn., DE-52 cellulose was dechlorinated, and then a hydrophilic chromatography column was used in combination with ultra performance liquid chromatography-tandem mass spectrometry. Positive ion mode, multiple reactions monitoring (MRM), and external standard method were used for quantify 17 kinds of hydrolyzed amino acids in in the Phyllanthus emblica Linn.. Results:The results show that DE-52 cellulose can effectively remove the chloride ions in the hydrolysis solution, and the hydrophilic column has good separation effect for leucine and isoleucine. In the concentration range of 0.000 3-2.0 μg·mL-1, the linear relationship of 17 amino acids was good, the detection limit ranged from 0.015 to 13.0 mg·kg-1, the recovery ranged from 73.8% to 98.8%, and the relative standard deviation (RSD) range was 3.25%-7.79%. Conclusion:This method has good reproducibility and high sensitivity, and is suitable for the determination of 17 kinds of hydrolyzed amino acids in the P.emblica Linn..
keywords:Hydrophilic chromatography  ultra performance liquid chromatography-tandem mass spectrometry  Phyllanthus emblica Linn.  hydrolyzed amino acid
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