Analysis and Evaluation of Multiple Types of Chemical Constituents in Stems and Leaves of Chrysanthemum Morifolium from Different Geographical Origins
Abstract:Objective:In order to analyze the multiple types of chemical constituents in the stems and leaves of Chrysanthemum morifolium from different geographical origins,and to provide the data support for their resource utilization.Methods:UV-Vis spectrophotometry was used for determining the total contents of flavonoids,phenols,and polysaccharides,and HPLC-ELSD method was used for analyzing monosaccharides and oligosaccharides in the stems and leaves of C. morifolium collected from 9 geographical origins.Results:The stems and leaves of C. morifolium from Anguo city,Hebei province (QJ-JY) had the highest content of total flavonoids and total phenols with the average content of (37.89±4.17) mg·g-1 and (17.12±2.00) mg·g-1 respectively. Compared with other geographical origins,the contents of total soluble polysaccharides were higher in the stems and leaves of C. morifolium from Zhengzhou city,Henan province (HBJ-JY) and Sheyang county,Jiangsu province (SYHBJ-JY) with the average content of (70.84±1.08) mg·g-1 and (70.32±2.90) mg·g-1 respectively. The monosaccharides contained in the stems and leaves of C.morifolium were mainly fructose and glucose,and the disaccharides were mainly sucrose. Among them,fructose was the main saccharides in the stems and leaves of C. morifolium. The stems and leaves of C. morifolium from Futianhe town,Macheng city (FBJ-JY) had the highest content of fructose with (94.22±6.20) mg·g-1. The result of correlation analysis showed that total flavonoids was positively correlated with total phenols,while they were negatively correlated with neutral polysaccharides. Moreover,there were a strong positive correlation between soluble carbohydrates (total polysaccharides,monosaccharides and oligosaccharides).Conclusion:The stems and leaves of C. morifolium were rich in these resourceful chemical compositions,and their contents were various in different geographical origins of this plant. The results provided a scientific basis for the utilization and industrial development of the stems and leaves of C. morifolium.