| 金莲花提取物代谢产物研究 |
| 投稿时间：2019-03-29 点此下载全文
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| 引用本文：刘斯琪,赵灿,丁鹏敏,陶志,赓迪,王如峰.金莲花提取物代谢产物研究[J].中国现代中药,2020,22(7):1027-1047 |
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| 摘要点击次数: 631 |
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| 基金项目:北京市自然科学基金面上项目（7172129） |
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| 中文摘要:目的：研究金莲花化学成分的体内代谢产物及代谢规律。方法：静脉注射方式给予大鼠金莲花提取物并在各时间段采血，利用高效液相色谱-电喷雾电离-串联质谱技术（LC-ESI-MS）对血清中的代谢产物进行研究。结果：根据LC-ESI-MS结果发现了23种原型成分及45种代谢产物。原型成分中有3种酚酸类物质、19种黄酮类物质、1种生物碱类物质。通过准分子离子峰及二级碎片得到了代谢产物可能的化学结构，分析了这些代谢产物的裂解途径，推测19种来源于酚酸类物质、20种来源于黄酮类物质、6种来源于生物碱类物质。结论：本研究探讨了金莲花提取物中化学成分的代谢规律，为其血液移行成分及药理作用的进一步研究奠定基础。 |
| 中文关键词:金莲花提取物 静脉注射 代谢产物 代谢规律 |
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| Study on Metabolites of Flos Trollii Extract in Rats |
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| Abstract:Objective：To study the metabolites and metabolism rules of the chemical constituents of Flos Trollii. Methods：The extract from Flos Trollii was given intravenously in rats and blood samples were collected at various time periods. The metabolites in serum were studied by high performance liquid chromatography electrospray ionization tandem mass spectrometry （LC-ESI-MS） technique. Results：23 Prototypic components including 3 phenolic acids，19 flavonoids and 1 alkaloid and 45 metabolites were identified by LC-ESI-MS. The possible chemical structures of metabolites were obtained from their quasi-molecular ion peaks and secondary fragments，then their cleavage pathways were analyzed. It was speculated that 19 metabolites came from phenolic acids，20 from flavonoids，and 6 from alkaloids. Conclusion：In this study，the metabolism of the chemical constituents in the extract of Flos Trollii was studied to provide a basis for the further study of its blood transitional components and pharmacological effects. |
| keywords:Flos Trollii extract intravenous injection metabolites metabolism rules |
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