| 新绿原酸的大鼠体内代谢产物的UPLC-Q-TOF MS鉴定 |
| 投稿时间：2019-10-30 点此下载全文
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| 引用本文：李洁,刘子菡,董凡,王喻淇,王少平,代龙,张加余,卢建秋.新绿原酸的大鼠体内代谢产物的UPLC-Q-TOF MS鉴定[J].中国现代中药,2020,22(7):1048-1055 |
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| 摘要点击次数: 786 |
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| 基金项目:中央本级重大增减支项目（2060302）；山东省青创人才引育创新研究团队项目；烟台市校地融合中药大健康产业化平台项目（2019XDRHXMPT18）；滨州医学院高层次人才科研启动基金（2019KYQD05） |
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| 中文摘要:目的：采用超高效液相色谱-四极杆-飞行时间质谱（UPLC-Q-TOF MS）高分辨质谱技术分析鉴定新绿原酸大鼠体内的代谢产物。方法：灌胃给药后，收集正常组和给药组大鼠的血浆、尿液和粪便。以固相萃取法处理生物样品后，采用Waters HSS T3 UPLC色谱柱（100 mm×2.1 mm，1.7 μm），0.1%甲酸水溶液（B）-乙腈（A）流动相系统进行梯度洗脱；在负离子模式下对生物样品进行分析。结果：最终根据所获得的精确相对分子质量、色谱保留行为、质谱裂解规律、特征碎片离子、对照品比对以及相关文献报道，共分析鉴定了包括原型药物在内的43个代谢产物。其主要代谢途径为异构化反应、水解反应、甲基化、葡萄糖醛酸化以及其复合反应。结论：应用UHPLC-Q-TOF MS高分辨液质联用技术能较为全面地阐明新绿原酸在大鼠体内的代谢情况，并为进一步的药效学以及绿原酸类物质的开发、利用提供借鉴。 |
| 中文关键词:新绿原酸 体内代谢 咖啡酸 奎尼酸 超高效液相色谱-四极杆-飞行时间质谱 |
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| Metabolite Identification of Neochlorogenic Acid in Rats Based on UPLC-Q-TOF MS |
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| Abstract:Objective：An efficient method of ultra-high performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry （UPLC-Q-TOF MS） was established to elucidate the in vivo metabolites of neochlorogenic acid in rats.Methods：Urine，plasma and feces samples were collected after oral gavage.After processing biological sample by solid-phase extraction，Waters ACQUITY HSS T3 column （100 mm×2.1 mm，1.7 μm） was used with 0.1% formic acid （A）-acetonitrile （B） solution as the mobile phase for gradient elution.The bio-samples were then analyzed by UPLC-Q-TOF equipped with a HESI ion source under negative ion mode. Results：According to the obtained accurate molecular weight，combined with chromatographic retention behavior，mass spectrometry cleavage，characteristic fragment ions，reference comparison，and related literature reports，a total of 43 metabolites.Main metabolic pathways contained isomerization，reduction reaction，methylation，glucosylation and their complicated reactions.Conclusion：The results showed that the metabolism of neochlorogenic could be comprehensively clarified by using UPLC-Q-TOF MS.And the study provided scientific evidence for the further study of the pharmacological activities of neochlorogenic acid. |
| keywords:neochlorogenic acid metabolite identification caffeic acid quinic acid UPLC-Q-TOF MS |
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