| 葛兰心宁软胶囊中绞股蓝皂苷A大鼠体内药代动力学研究 |
| 投稿时间：2019-09-05 点此下载全文
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| 引用本文：胡小虎,孙文军,高昕,王夕静.葛兰心宁软胶囊中绞股蓝皂苷A大鼠体内药代动力学研究[J].中国现代中药,2020,22(7):1066-1071 |
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| 中文摘要:目的：应用超高效液相色谱-质谱联用分析方法（UPLC-MS），建立葛兰心宁软胶囊中绞股蓝皂苷A的大鼠血浆中的定量分析方法。方法：测定大鼠口服葛兰心宁软胶囊内容物后，血液中绞股蓝皂苷A的血药浓度随时间的动态变化，用药动学统计软件DAS 3.0 进行统计，计算药代动力学参数。结果：线性范围为10~500 ng·mL-1（r = 0.998 4）；高、中、低浓度的日内ＲSD分别为2.7%、2.6%、4.2%；日间ＲSD分别为2.4%、2.8%、3.1%；基质效应84.9%~92.1%，RSD<5%；回收率>80.0%。口服葛兰心宁软胶囊，绞股蓝皂苷A在大鼠体内的药代动力学参数tmax为0.75 h，t1/2为（6.247±2.039）h，药时曲线下面积（AUC）为（2562±252） μg·L-1·h-1。结论：绞股蓝皂苷A的药动学行为特征为口服快速吸收、达峰时间快、快速消除。 |
| 中文关键词:绞股蓝皂苷A 药代动力学 液质联用 血药浓度 葛兰心宁软胶囊 |
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| Pharmacokinetics of Gypenoside A in Glanxinning Soft Capsules in Rats |
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| Abstract:Objective：An in vivo method for the determination of gypenoside A in Glanxinning soft capsules was established by ultra-high performance liquid chromatography-mass spectrometry（UPLC-MS）. Methods：The plasma concentration of gypenoside A in rats was determined by UPLC-MS after intragastric administration of Glanxinning soft capsule. The pharmacokinetic parameters of gypenoside A in rats were calculated by DAS 3.0 software. Results：The results showed that the linear range was 10-500 ng·mL-1 （r=0.998 4）；RSD was 2.7%，2.6% and 4.2% in high，medium and low concentrations；RSD was 2.4%，2.8% and 3.1% in daytime；matrix effect was 84.9%-92.1%，RSD was less than 5%；recovery was more than 80.0%. The oral pharmacokinetic parameters of gypenoside A in rats were Tmax 0.75 h，t1/2 of （6.247±2.039）h，and AUC of （2562±252） μg·L-1·h-1. Conclusion：The results showed that the pharmacokinetic characteristics of gypenoside A were fast absorption，distribution，peak time and elimination. |
| keywords:gypenoside A pharmacokinetics LC-MS/MS blood concentration Glanxinning soft capsule |
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