| UPLC-QTOF-MS技术在摩罗丹浓缩丸质量稳定性评价中的应用 |
| 投稿时间：2019-08-15 点此下载全文
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| 引用本文：刘会云,田颖瑞,睢建亮,王梦蕾,殷苗苗,王珍.UPLC-QTOF-MS技术在摩罗丹浓缩丸质量稳定性评价中的应用[J].中国现代中药,2020,22(9):1539-1544 |
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| 基金项目:河北省自然科学基金青年项目（C2019402141）；河北省高等学校科学技术研究项目（QN2019215）；邯郸市科学技术研究与发展计划项目（1727201061） |
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| 中文摘要:目的：利用液质联用技术对中药所含化学成分进行分析，探究其在中药质量稳定性评价中的应用。方法：采用超高效液相色谱-四级杆串联飞行时间质谱法（UPLC-QTOF-MS）对摩罗丹浓缩丸样品进行测定，通过对比分析10个不同批次摩罗丹浓缩丸的检测结果，并结合主成分分析（PCA），对摩罗丹浓缩丸的质量稳定性进行评价。结果：利用UPLC-QTOF-MS技术，摩罗丹浓缩丸中共鉴定出367种化学成分。通过对比分析10个不同批次摩罗丹浓缩丸所含化学成分种类及数量，得出不同批次样品所含化学成分的平均重复率达到92%，并通过PCA证明其整体质量稳定性较好。结论：利用液质联用技术结合PCA为摩罗丹浓缩丸的整体质量评价提供参考，并为中药作用物质成分分析提供新的检测方法和思路。 |
| 中文关键词:摩罗丹浓缩丸 超高效液相色谱-四级杆串联飞行时间质谱法 质量稳定性 主成分分析 |
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| Application of UPLC-QTOF-MS Technology in Quality Stability Evaluation of Moluodan Concentrated Pill |
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| Abstract:Objective：To analyze the chemical composition of traditional Chinese medicines by UPLC-QTOF-MS，and explore its application in the evaluation of quality stability of traditional Chinese medicines. Methods：Moluodan concentrated pill samples were determined by ultra-high-UPLC-QTOF-MS，combined with principal component analysis，the quality stability of Moluodan concentrated pill was evaluated by comparing and analyzing of the 10 different batches of Moluodan concentrated pill. Results：A total of 367 chemical constituents were identified in Moluodan concentrated pill by UPLC-QTOF-MS. By comparing and analyzing the chemical composition types and quantities of 10 different batches of Moluodan concentrated pill，it was concluded that the average repetition rate of chemical components in different batches of drugs reached 92%，and the overall quality was stable by principal component analysis. Conclusion：In this study，liquid chromatography-mass spectrometry combined with PCA analysis laid a foundation for the overall quality evaluation of Moluodan concentrated pill，and provided new detection methods and ideas for the analysis of the constituents of Chinese medicines. |
| keywords:Moluodan concentrated pill UPLC-QTOF-MS quality stability PCA |
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